PMID- 29163648
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1728-1997 (Print)
IS  - 2252-0589 (Electronic)
IS  - 2252-0589 (Linking)
VI  - 18
IP  - 3
DP  - 2017 Summer
TI  - Fiber gene based molecular and biological characterization of 
      hydropericardium-hepatitis syndrome associated avian adenoviruses.
PG  - 190-196
AB  - This study was designed to perform biological and molecular characterization of 
      avian adenoviruses (AAVs) recovered from suspected cases of 
      hydropericardium-hepatitis syndrome (HHS) in commercial poultry. Initially the 
      samples were screened by Agar Gel Precipitation Test (AGPT) for the presence of 
      AAVs followed by its confirmation and typing through polymerase chain reaction 
      (PCR) focusing on already reported serotypes AAV-4, AAV-8 and AAV-10 elsewhere. 
      These PCR-positive samples were further subjected to amplification of fiber gene, 
      followed by conducting restriction fragment length polymorphism (RFLP) using 
      restriction enzyme Alu. The selected isolates were further propagated through 
      cell culture and pathogenic potential of selected isolates was determined by 
      infecting chickens. In this study, out of a total 190 samples, 57.8% of suspected 
      cases were found positive for AAV presence through AGPT while sub-type 
      identification using PCR revealed 46.3% for these viruses belonging to AAV-4, 
      41.8% to AAV-8 and 11.8% showed co-infection of AAV-4 and AAV-8. AAV-10 was not 
      detected in any of the tested samples. On the basis of RFLP pattern, AAV-4 
      isolates were further divided into four sub-groups (A-D) while AAV-8 isolates had 
      identical RFLP pattern. To further evaluate the pathogenic potential of these 
      sub-groups of AAV-4 isolates, specific pathogen free (SPF) chicks were challenged 
      with selected isolates belonging to each of the sub-groups, resulting in variable 
      pattern of pathogenicity. It is concluded that any variation in the fiber gene of 
      AAV-4 isolates may affect its pathogenicity and eventually specificity of the 
      vaccines used against such infections. Therefore, regular monitoring of the 
      circulating AAV serotypes may be helpful in understanding the pathogenic 
      potential of emerging AAVs, which may lead to development of more effective 
      response strategies accordingly.
FAU - Yasmeen, S
AU  - Yasmeen S
AD  - Mphil in Animal Genomics and Biotechnology, National Reference Lab for Poultry 
      Diseases, Animal Sciences Institute, National Agricultural Research Centre, 
      Islamabad-45500, Pakistan.
FAU - Siddique, N
AU  - Siddique N
AD  - National Reference Lab for Poultry Diseases, Animal Sciences Institute, National 
      Agricultural Research Centre, Islamabad-45500, Pakistan.
FAU - Athar Abbas, M
AU  - Athar Abbas M
AD  - National Reference Lab for Poultry Diseases, Animal Sciences Institute, National 
      Agricultural Research Centre, Islamabad-45500, Pakistan.
FAU - Ali, A
AU  - Ali A
AD  - National Reference Lab for Poultry Diseases, Animal Sciences Institute, National 
      Agricultural Research Centre, Islamabad-45500, Pakistan.
FAU - Rafique, S
AU  - Rafique S
AD  - Ph.D. Scholar, National Reference Lab for Poultry Diseases, Animal Sciences 
      Institute, National Agricultural Research Centre, Islamabad-45500, Pakistan.
FAU - Rashid, F
AU  - Rashid F
AD  - National Reference Lab for Poultry Diseases, Animal Sciences Institute, National 
      Agricultural Research Centre, Islamabad-45500, Pakistan.
FAU - Shah, A U
AU  - Shah AU
AD  - MSc in Biotechnology, National Reference Lab for Poultry Diseases, Animal 
      Sciences Institute, National Agricultural Research Centre, Islamabad-45500, 
      Pakistan.
FAU - Mehmood, F
AU  - Mehmood F
AD  - Mphil in Microbiology, National Reference Lab for Poultry Diseases, Animal 
      Sciences Institute, National Agricultural Research Centre, Islamabad-45500, 
      Pakistan.
FAU - Begum, I
AU  - Begum I
AD  - Mphil in Biology, National Reference Lab for Poultry Diseases, Animal Sciences 
      Institute, National Agricultural Research Centre, Islamabad-45500, Pakistan.
FAU - Javaid, T
AU  - Javaid T
AD  - Al-Ferooz Poultry Clinic, Rawalpindi-46000, Pakistan.
FAU - Jaffery, S M H
AU  - Jaffery SMH
AD  - Jaffmac Consultant Services, Lahore, Pakistan.
FAU - Ali, R
AU  - Ali R
AD  - Remount Veterinary and Farms Directorate, GHQ, Rawalpindi, Pakistan.
FAU - Naeem, Kh
AU  - Naeem K
AD  - National Reference Lab for Poultry Diseases, Animal Sciences Institute, National 
      Agricultural Research Centre, Islamabad-45500, Pakistan.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Vet Res
JT  - Iranian journal of veterinary research
JID - 101660030
PMC - PMC5674442
OTO - NOTNLM
OT  - Avian adenoviruses
OT  - Hydropericardium syndrome
OT  - Hydropericardium-hepatitis syndrome
OT  - Inclusion body hepatitis
COIS- The authors of the paper affirm that there is no conflict of interest concerning 
      the publication of the current paper.
EDAT- 2017/11/23 06:00
MHDA- 2017/11/23 06:01
PMCR- 2017/07/01
CRDT- 2017/11/23 06:00
PHST- 2017/11/23 06:00 [entrez]
PHST- 2017/11/23 06:00 [pubmed]
PHST- 2017/11/23 06:01 [medline]
PHST- 2017/07/01 00:00 [pmc-release]
AID - ijvr-18-190 [pii]
PST - ppublish
SO  - Iran J Vet Res. 2017 Summer;18(3):190-196.