PMID- 29163819
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220311
IS  - 1949-2553 (Electronic)
IS  - 1949-2553 (Linking)
VI  - 8
IP  - 52
DP  - 2017 Oct 27
TI  - Exploring the model of PC12 apoptosis induced by OGSD/R through in vitro 
      experiments.
PG  - 90176-90184
LID - 10.18632/oncotarget.21623 [doi]
AB  - AIMS: To explicit cell apoptosis trend in PC12 oxygen-glucose-serum 
      deprivation/restoration (OGSD/R) model and provide experimental bases for neural 
      cell simulation in ischemia reperfusion injury in vitro. METHODS: OGSD/R model 
      was constructed using the passage PC12 cells in vitro. The profile of cell 
      apoptosis was estimated by DAPI staining, Annexin V-FITC and terminal 
      deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, as well as the 
      levels of apoptosis-related proteins, including procaspase-3 and caspase-12. 
      RESULTS: PC12 apoptosis was induced by OGSD and aggravated after restoration. 
      CCK8 assay indicated that cell activity reached minimum after 1h of 
      oxygen-glucose-serum restoration (OGR). DAPI staining suggested that apoptosis 
      was the most serious after 1h of OGR, causing apoptotic cell nucleus pyknosis, 
      particle spot formation, and fracture of cells with serious apoptosis forming 
      pieces, and nucleus disintegration. The percentage of apoptotic cells exhibited 
      increased trend after restoration, and reached the highest at 1h of OGR. 
      Moreover, the expression of procaspase-3 and caspase-12 were extremely enhanced 
      after OGD, especially 1h after OGR. CONCLUSIONS: PC12 apoptosis is induced by 
      OGSD and aggravated after restoration. The apoptosis of PC12 reaches the highest 
      at 1h after OGR, which may provide experimental bases for spinal cord ischemia 
      reperfusion injury treatment.
FAU - Sun, Yanqing
AU  - Sun Y
AD  - Department of Spine, Changzheng Hospital, Second Military Medical University, 
      Shanghai 200003, China.
FAU - Zhu, Wei
AU  - Zhu W
AD  - Department of Spine, Changzheng Hospital, Second Military Medical University, 
      Shanghai 200003, China.
FAU - Zhou, Shengyuan
AU  - Zhou S
AD  - Department of Spine, Changzheng Hospital, Second Military Medical University, 
      Shanghai 200003, China.
FAU - Wang, Zhiwei
AU  - Wang Z
AD  - Department of Spine, Changzheng Hospital, Second Military Medical University, 
      Shanghai 200003, China.
FAU - Chen, Xiongsheng
AU  - Chen X
AD  - Department of Spine, Changzheng Hospital, Second Military Medical University, 
      Shanghai 200003, China.
FAU - Jia, Lianshun
AU  - Jia L
AD  - Department of Spine, Changzheng Hospital, Second Military Medical University, 
      Shanghai 200003, China.
LA  - eng
PT  - Journal Article
DEP - 20171004
PL  - United States
TA  - Oncotarget
JT  - Oncotarget
JID - 101532965
PMC - PMC5685740
OTO - NOTNLM
OT  - PC12
OT  - apoptosis
OT  - ischemia reperfusion
OT  - oxygen-glucose-serum deprivation/restoration
COIS- CONFLICTS OF INTEREST The authors report no conflicts of interest in this work.
EDAT- 2017/11/23 06:00
MHDA- 2017/11/23 06:01
PMCR- 2017/10/27
CRDT- 2017/11/23 06:00
PHST- 2017/03/01 00:00 [received]
PHST- 2017/08/06 00:00 [accepted]
PHST- 2017/11/23 06:00 [entrez]
PHST- 2017/11/23 06:00 [pubmed]
PHST- 2017/11/23 06:01 [medline]
PHST- 2017/10/27 00:00 [pmc-release]
AID - 21623 [pii]
AID - 10.18632/oncotarget.21623 [doi]
PST - epublish
SO  - Oncotarget. 2017 Oct 4;8(52):90176-90184. doi: 10.18632/oncotarget.21623. 
      eCollection 2017 Oct 27.