PMID- 29164291 OWN - NLM STAT- MEDLINE DCOM- 20180823 LR - 20181113 IS - 1432-0991 (Electronic) IS - 0343-8651 (Print) IS - 0343-8651 (Linking) VI - 75 IP - 4 DP - 2018 Apr TI - Bacterial Diversity in Traditional Doogh in Comparison to Industrial Doogh. PG - 386-393 LID - 10.1007/s00284-017-1392-x [doi] AB - Forty-four samples of traditional Doogh and yoghurt were collected from 13 regions of 4 provinces in west of Iran (13 area) and analyzed using molecular methods including PCR, denaturing gradient gel electrophoresis (DGGE) of 16S rDNA, and sequencing. Moreover, collected samples as well as samples from industrially Doogh were analyzed with quantitative real-time PCR (RT-PCR). Analyzed 16S rRNA gene sequences of Doogh samples could be allocated to the presence of Lactobacillus spp. The typical yoghurt starter culture bacteria included four different Lactobacillus species with possible probiotic properties, L. acidophilus, L. helveticus, L. kefiranofaciens, and L. amylovorus. DGGE of traditional Doogh and yoghurt and RT-PCR of traditional Doogh and yoghurt and also industrial Doogh samples demonstrated that traditional Doogh and yoghurt show a higher abundance of total bacteria and lactobacilli and a higher bacterial diversity, respectively. Considering diversity and higher probiotic bacteria content in traditional Doogh, consumers' healthiness in tribes and villages could be promoted with these indigenous products. FAU - Sayevand, Hamid Reza AU - Sayevand HR AD - Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, UZAII;2D541, 1090, Vienna, Austria. FAU - Bakhtiary, Farzaneh AU - Bakhtiary F AD - Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, UZAII;2D541, 1090, Vienna, Austria. FAU - Pointner, Angelika AU - Pointner A AD - Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, UZAII;2D541, 1090, Vienna, Austria. FAU - Remely, Marlene AU - Remely M AD - Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, UZAII;2D541, 1090, Vienna, Austria. FAU - Hippe, Berit AU - Hippe B AD - Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, UZAII;2D541, 1090, Vienna, Austria. FAU - Hosseini, Hedayat AU - Hosseini H AD - Faculty of Nutrition Sciences & Food Technology, National Nutrition & Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Haslberger, Alexander AU - Haslberger A AD - Department of Nutritional Sciences, University of Vienna, Althanstrasse 14, UZAII;2D541, 1090, Vienna, Austria. alexander.haslberger@univie.ac.at. LA - eng PT - Comparative Study PT - Journal Article DEP - 20171121 PL - United States TA - Curr Microbiol JT - Current microbiology JID - 7808448 RN - 0 (DNA, Bacterial) RN - 0 (DNA, Ribosomal) RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Biodiversity MH - Cultured Milk Products/*microbiology MH - DNA, Bacterial/genetics MH - DNA, Ribosomal/genetics MH - Food Microbiology MH - Iran MH - Lactobacillus/classification/genetics/*isolation & purification/metabolism MH - Phylogeny MH - RNA, Ribosomal, 16S/genetics MH - Yogurt/*microbiology PMC - PMC5842502 EDAT- 2017/11/23 06:00 MHDA- 2018/08/24 06:00 PMCR- 2017/11/21 CRDT- 2017/11/23 06:00 PHST- 2017/06/30 00:00 [received] PHST- 2017/11/07 00:00 [accepted] PHST- 2017/11/23 06:00 [pubmed] PHST- 2018/08/24 06:00 [medline] PHST- 2017/11/23 06:00 [entrez] PHST- 2017/11/21 00:00 [pmc-release] AID - 10.1007/s00284-017-1392-x [pii] AID - 1392 [pii] AID - 10.1007/s00284-017-1392-x [doi] PST - ppublish SO - Curr Microbiol. 2018 Apr;75(4):386-393. doi: 10.1007/s00284-017-1392-x. Epub 2017 Nov 21.