PMID- 29165345
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 6
IP  - 4
DP  - 2017 Nov 22
TI  - Activity In Vitro of Clotrimazole against Canine Methicillin-Resistant and 
      Susceptible Staphylococcus pseudintermedius.
LID - 10.3390/antibiotics6040029 [doi]
LID - 29
AB  - Emergence of multidrug-resistance in Staphylococcus pseudintermedius (SP) has 
      increased interest in topical therapy as an alternative to systemic antibiotics 
      in canine pyoderma. The antifungal imidazole, clotrimazole, is contained in 
      numerous licensed canine ear preparations. Its in vitro activity against SP has 
      not been evaluated, although previous studies have shown that the related 
      imidazole, miconazole, has significant anti-staphylococcal efficacy. We therefore 
      determined minimum inhibitory concentrations (MICs) of clotrimazole amongst 50 SP 
      isolates (25 methicillin-resistant [MR]SP and susceptible [MS]SP) collected from 
      dogs in Germany during 2010-2011 using an agar dilution method (CLSI VET01-A4). 
      MICs amongst MRSP and MSSP were comparable (MIC(50) and MIC(90) = 1mg/L for both 
      groups, p = 0.317); overall, 49 isolates had MIC = 1 mg/L and one had MIC = 0.5 
      mg/L. The relatively low MICs obtained in this study are likely to be exceeded by 
      topical therapy and thus further clinical evaluation of clotrimazole use in 
      canine superficial pyoderma and otitis externa caused by MRSP and MSSP is now 
      warranted.
FAU - Frosini, Sian-Marie
AU  - Frosini SM
AD  - Clinical Science and Services, Royal Veterinary College, Hawkshead Lane, North 
      Mymms, Hatfield AL9 7TA, UK. sfrosini@rvc.ac.uk.
FAU - Bond, Ross
AU  - Bond R
AD  - Clinical Science and Services, Royal Veterinary College, Hawkshead Lane, North 
      Mymms, Hatfield AL9 7TA, UK. rbond@rvc.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20171122
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC5745472
OTO - NOTNLM
OT  - Staphylococcus pseudintermedius
OT  - canine
OT  - clotrimazole
OT  - otitis externa
OT  - topical therapy
COIS- The authors declare no conflict of interest. S.-M.F. ( Clark) was previously in 
      receipt of an iCASE PhD studentship from BBSRC and Dechra Veterinary Products but 
      neither of these sponsors had any role in the design of the study; in the 
      collection, analyses, or interpretation of data; in the writing of the 
      manuscript, and in the decision to publish the results.
EDAT- 2017/11/23 06:00
MHDA- 2017/11/23 06:01
PMCR- 2017/11/22
CRDT- 2017/11/23 06:00
PHST- 2017/10/26 00:00 [received]
PHST- 2017/11/17 00:00 [revised]
PHST- 2017/11/18 00:00 [accepted]
PHST- 2017/11/23 06:00 [entrez]
PHST- 2017/11/23 06:00 [pubmed]
PHST- 2017/11/23 06:01 [medline]
PHST- 2017/11/22 00:00 [pmc-release]
AID - antibiotics6040029 [pii]
AID - antibiotics-06-00029 [pii]
AID - 10.3390/antibiotics6040029 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2017 Nov 22;6(4):29. doi: 10.3390/antibiotics6040029.