PMID- 29166392
OWN - NLM
STAT- MEDLINE
DCOM- 20171211
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 11
DP  - 2017
TI  - Intestinal Ralstonia pickettii augments glucose intolerance in obesity.
PG  - e0181693
LID - 10.1371/journal.pone.0181693 [doi]
LID - e0181693
AB  - An altered intestinal microbiota composition has been implicated in the 
      pathogenesis of metabolic disease including obesity and type 2 diabetes mellitus 
      (T2DM). Low grade inflammation, potentially initiated by the intestinal 
      microbiota, has been suggested to be a driving force in the development of 
      insulin resistance in obesity. Here, we report that bacterial DNA is present in 
      mesenteric adipose tissue of obese but otherwise healthy human subjects. 
      Pyrosequencing of bacterial 16S rRNA genes revealed that DNA from the 
      Gram-negative species Ralstonia was most prevalent. Interestingly, fecal 
      abundance of Ralstonia pickettii was increased in obese subjects with 
      pre-diabetes and T2DM. To assess if R. pickettii was causally involved in 
      development of obesity and T2DM, we performed a proof-of-concept study in 
      diet-induced obese (DIO) mice. Compared to vehicle-treated control mice, R. 
      pickettii-treated DIO mice had reduced glucose tolerance. In addition, 
      circulating levels of endotoxin were increased in R. pickettii-treated mice. In 
      conclusion, this study suggests that intestinal Ralstonia is increased in obese 
      human subjects with T2DM and reciprocally worsens glucose tolerance in DIO mice.
FAU - Udayappan, Shanthadevi D
AU  - Udayappan SD
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Kovatcheva-Datchary, Petia
AU  - Kovatcheva-Datchary P
AD  - Wallenberg Laboratory, University of Gothenburg, Sahlgrenska University Hospital, 
      Gothenburg, Sweden.
FAU - Bakker, Guido J
AU  - Bakker GJ
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Havik, Stefan R
AU  - Havik SR
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Herrema, Hilde
AU  - Herrema H
AUID- ORCID: 0000-0002-0112-6348
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Cani, Patrice D
AU  - Cani PD
AD  - Universite catholique de Louvain, WELBIO (Walloon Excellence in Life sciences and 
      BIOtechnology), Louvain Drug Research Institute, Brussels, Belgium.
FAU - Bouter, Kristien E
AU  - Bouter KE
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Belzer, Clara
AU  - Belzer C
AD  - Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
FAU - Witjes, Julia J
AU  - Witjes JJ
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Vrieze, Anne
AU  - Vrieze A
AD  - Department of Internal Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - de Sonnaville, Eleanore Susanne Victoria
AU  - de Sonnaville ESV
AD  - Department of Internal Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Chaplin, Alice
AU  - Chaplin A
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - van Raalte, Daniel H
AU  - van Raalte DH
AD  - Diabetes Center, Department of Internal medicine, VU University Medical Center, 
      Amsterdam, The Netherlands.
AD  - ICAR, VU University Medical Center, Amsterdam, The Netherlands.
FAU - Aalvink, Steven
AU  - Aalvink S
AD  - Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
FAU - Dallinga-Thie, Geesje M
AU  - Dallinga-Thie GM
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Heilig, Hans G H J
AU  - Heilig HGHJ
AD  - Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
FAU - Bergstrom, Goran
AU  - Bergstrom G
AD  - Wallenberg Laboratory, University of Gothenburg, Sahlgrenska University Hospital, 
      Gothenburg, Sweden.
FAU - van der Meij, Suzan
AU  - van der Meij S
AD  - Department of Surgery, Flevo Hospital, Almere, The Netherlands.
FAU - van Wagensveld, Bart A
AU  - van Wagensveld BA
AD  - Department of Surgery, Sint Lucas Andreas Hospital, Amsterdam, The Netherlands.
FAU - Hoekstra, Joost B L
AU  - Hoekstra JBL
AD  - Department of Internal Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Holleman, Frits
AU  - Holleman F
AD  - Department of Internal Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Stroes, Erik S G
AU  - Stroes ESG
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
FAU - Groen, Albert K
AU  - Groen AK
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
AD  - Department of Pediatrics, University Medical Center Groningen, University of 
      Groningen, Groningen, The Netherlands.
FAU - Backhed, Fredrik
AU  - Backhed F
AD  - Wallenberg Laboratory, University of Gothenburg, Sahlgrenska University Hospital, 
      Gothenburg, Sweden.
AD  - Novo Nordisk Foundation Center for Basic Metabolic Research, Section for 
      Metabolic Receptology and Enteroendocrinology, Faculty of Health Sciences, 
      University of Copenhagen, Copenhagen, Denmark.
FAU - de Vos, Willem M
AU  - de Vos WM
AD  - Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
AD  - RPU Immunobiology, University of Helsinki, Helsinki, Finland.
FAU - Nieuwdorp, Max
AU  - Nieuwdorp M
AD  - Department of Vascular Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
AD  - Wallenberg Laboratory, University of Gothenburg, Sahlgrenska University Hospital, 
      Gothenburg, Sweden.
AD  - Department of Internal Medicine, Academic Medical Center, Amsterdam, The 
      Netherlands.
AD  - Diabetes Center, Department of Internal medicine, VU University Medical Center, 
      Amsterdam, The Netherlands.
AD  - ICAR, VU University Medical Center, Amsterdam, The Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20171122
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Bacterial)
SB  - IM
EIN - PLoS One. 2018 Jan 30;13(1):e0192339. PMID: 29381738
MH  - Aged
MH  - Animals
MH  - DNA, Bacterial/analysis
MH  - Diabetes Mellitus, Type 2/complications/microbiology
MH  - Diet, High-Fat
MH  - Feces/microbiology
MH  - Female
MH  - Glucose Intolerance/*complications/*microbiology
MH  - Gram-Negative Bacterial Infections/*microbiology/pathology
MH  - Humans
MH  - Inflammation/complications/pathology
MH  - Intestines/*microbiology/pathology
MH  - Intra-Abdominal Fat/microbiology/pathology
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Obesity/*complications/*microbiology
MH  - Ralstonia pickettii/*physiology
PMC - PMC5699813
COIS- Competing Interests: We have the following interests. This study was partly 
      funded by AFA insurances. M.N. and W.M.deV. are founders, own equity and are in 
      the Scientific Advisory Board of Caelus Pharmaceuticals, the Netherlands; WMdV is 
      in the Scientific Advisory Board of Chr Hansen Horsholm Danmark and the M.N. and 
      W.M.deV. are founders, own equity and are in the Scientific Advisory Board of 
      Caelus Pharmaceutical(NIHS) Lausanne Switzerland. F.B. is a founder of MetaboGen 
      AB, Sweden. F.B. and G.B own equity in MetaboGen AB, Sweden. None of these are 
      directly relevant to the current paper. There are no patents, products in 
      development or marketed products to declare. This does not alter our adherence to 
      all the PLOS ONE policies on sharing data and materials, as detailed online in 
      the guide for authors.
EDAT- 2017/11/23 06:00
MHDA- 2017/12/12 06:00
PMCR- 2017/11/22
CRDT- 2017/11/23 06:00
PHST- 2017/01/13 00:00 [received]
PHST- 2017/07/04 00:00 [accepted]
PHST- 2017/11/23 06:00 [entrez]
PHST- 2017/11/23 06:00 [pubmed]
PHST- 2017/12/12 06:00 [medline]
PHST- 2017/11/22 00:00 [pmc-release]
AID - PONE-D-17-01669 [pii]
AID - 10.1371/journal.pone.0181693 [doi]
PST - epublish
SO  - PLoS One. 2017 Nov 22;12(11):e0181693. doi: 10.1371/journal.pone.0181693. 
      eCollection 2017.