PMID- 29168776
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240327
IS  - 1996-1944 (Print)
IS  - 1996-1944 (Electronic)
IS  - 1996-1944 (Linking)
VI  - 10
IP  - 12
DP  - 2017 Nov 23
TI  - Enhancement of Osteoblastic-Like Cell Activity by Glow Discharge Plasma Surface 
      Modified Hydroxyapatite/beta-Tricalcium Phosphate Bone Substitute.
LID - 10.3390/ma10121347 [doi]
LID - 1347
AB  - Glow discharge plasma (GDP) treatments of biomaterials, such as 
      hydroxyapatite/beta-tricalcium phosphate (HA/beta-TCP) composites, produce surfaces 
      with fewer contaminants and may facilitate cell attachment and enhance bone 
      regeneration. Thus, in this study we used argon glow discharge plasma (Ar-GDP) 
      treatments to modify HA/beta-TCP particle surfaces and investigated the physical and 
      chemical properties of the resulting particles (HA/beta-TCP + Ar-GDP). The HA/beta-TCP 
      particles were treated with GDP for 15 min in argon gas at room temperature under 
      the following conditions: power: 80 W; frequency: 13.56 MHz; pressure: 100 mTorr. 
      Scanning electron microscope (SEM) observations showed similar rough surfaces of 
      HA/beta-TCP + Ar-GDP HA/beta-TCP particles, and energy dispersive spectrometry analyses 
      showed that HA/beta-TCP surfaces had more contaminants than HA/beta-TCP + Ar-GDP 
      surfaces. Ca/P mole ratios in HA/beta-TCP and HA/beta-TCP + Ar-GDP were 1.34 and 1.58, 
      respectively. Both biomaterials presented maximal intensities of X-ray 
      diffraction patterns at 27 degrees  with 600 a.u. At 25 degrees  and 40 degrees , HA/beta-TCP + Ar-GDP and 
      HA/beta-TCP particles had peaks of 200 a.u., which are similar to XRD intensities of 
      human bone. In subsequent comparisons, MG-63 cell viability and differentiation 
      into osteoblast-like cells were assessed on HA/beta-TCP and HA/beta-TCP + Ar-GDP 
      surfaces, and Ar-GDP treatments led to improved cell growth and alkaline 
      phosphatase activities. The present data indicate that GDP surface treatment 
      modified HA/beta-TCP surfaces by eliminating contaminants, and the resulting graft 
      material enhanced bone regeneration.
FAU - Salamanca, Eisner
AU  - Salamanca E
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 
      110, Taiwan. eisnergab@hotmail.com.
FAU - Pan, Yu-Hwa
AU  - Pan YH
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 
      110, Taiwan. shalom.dc@msa.hinet.net.
AD  - Department of Dentistry, Chang Gung Memorial Hospital, Taipei 105, Taiwan. 
      shalom.dc@msa.hinet.net.
AD  - Graduate Institute of Dental & Craniofacial Science, Chang Gung University, 
      Taoyuan 333, Taiwan. shalom.dc@msa.hinet.net.
AD  - School of Dentistry, College of Medicine, China Medical University, Taichung 404, 
      Taiwan. shalom.dc@msa.hinet.net.
FAU - Tsai, Aileen I
AU  - Tsai AI
AD  - Department of Dentistry, Chang Gung Memorial Hospital, Taipei 105, Taiwan. 
      ait001@adm.cgmh.org.tw.
FAU - Lin, Pei-Ying
AU  - Lin PY
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 
      110, Taiwan. payinglin53@gmail.com.
FAU - Lin, Ching-Kai
AU  - Lin CK
AD  - Department of Dentistry, Chang Gung Memorial Hospital, Taipei 105, Taiwan. 
      philipcklin@msn.com.
FAU - Huang, Haw-Ming
AU  - Huang HM
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 
      110, Taiwan. hhm@tmu.edu.tw.
AD  - Graduate Institute of Biomedical Materials & Tissue Engineering, College of Oral 
      Medicine, Taipei Medical University, Taipei 110, Taiwan. hhm@tmu.edu.tw.
FAU - Teng, Nai-Chia
AU  - Teng NC
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 
      110, Taiwan. dianaten@tmu.edu.tw.
AD  - Dental Department, Taipei Medical University Hospital, Taipei 110, Taiwan. 
      dianaten@tmu.edu.tw.
FAU - Wang, Peter D
AU  - Wang PD
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 
      110, Taiwan. dpw1@tmu.edu.tw.
AD  - Dental Department, Taipei Medical University Hospital, Taipei 110, Taiwan. 
      dpw1@tmu.edu.tw.
FAU - Chang, Wei-Jen
AU  - Chang WJ
AD  - School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 
      110, Taiwan. cweijen1@tmu.edu.tw.
AD  - Dental Department, Taipei Medical University, Shuang-Ho Hospital, Taipei 235, 
      Taiwan. cweijen1@tmu.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20171123
PL  - Switzerland
TA  - Materials (Basel)
JT  - Materials (Basel, Switzerland)
JID - 101555929
PMC - PMC5744282
OTO - NOTNLM
OT  - HA/beta-TCP
OT  - argon glow discharge plasma
OT  - cell viability
OT  - differentiation
OT  - guided bone regeneration
OT  - osteoconduction
COIS- The authors declare no conflict of interest.
EDAT- 2017/11/24 06:00
MHDA- 2017/11/24 06:01
PMCR- 2017/11/23
CRDT- 2017/11/24 06:00
PHST- 2017/10/05 00:00 [received]
PHST- 2017/11/11 00:00 [revised]
PHST- 2017/11/21 00:00 [accepted]
PHST- 2017/11/24 06:00 [entrez]
PHST- 2017/11/24 06:00 [pubmed]
PHST- 2017/11/24 06:01 [medline]
PHST- 2017/11/23 00:00 [pmc-release]
AID - ma10121347 [pii]
AID - materials-10-01347 [pii]
AID - 10.3390/ma10121347 [doi]
PST - epublish
SO  - Materials (Basel). 2017 Nov 23;10(12):1347. doi: 10.3390/ma10121347.