PMID- 29169695
OWN - NLM
STAT- MEDLINE
DCOM- 20181127
LR  - 20181127
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 120
IP  - 1
DP  - 2018 Jan
TI  - Immunohistochemical analysis of the distribution of molecules involved in ionic 
      and pH regulation in the lancelet Branchiostoma floridae (Hubbs, 1922).
PG  - 33-40
LID - S0065-1281(17)30206-4 [pii]
LID - 10.1016/j.acthis.2017.10.011 [doi]
AB  - The aim of present work is to analyse the distribution of carbonic anhydrase II 
      (CAII), cystic fibrosis transmembrane regulator (CFTR), vacuolar-type H(+)-ATPase 
      (V-H(+)-ATPase), Na(+)/K(+) ATPase, Na(+)/H(+) exchanger (NHE) and SLC26A6 
      (solute carrier family 26, member 6), also known as pendrin protein, in the 
      lancelet Branchiostoma floridae in order to go in depth in the evolution of 
      osmoregulation and pH regulation in Chordates. In view of their phylogenetic 
      position, lancelets may indeed provide a critical point of reference for studies 
      on osmoregulation evolution in Chordates. The results of present work 
      demonstrated that, except to Na(+)/K(+) ATPase that is strongly expressed in 
      nephridia only, all the other studied molecules are abundantly present in skin, 
      coelomic epithelium, renal papillae and nephridia and hepatic coecum. Thus, it is 
      possible to hypothesize that also in lancelet, as in fish, these organs are 
      involved in pH control and ionic regulation. In the digestive tract of B. 
      floridae, the intestine epithelium was weakly immune-reactive to all tested 
      antibodies, while the hepatic coecum showed an intense immunoreactivity to all 
      molecules. Since in amphioxus the hepatic coecum functions simultaneously as 
      stomach, liver and pancreas, these immunohistochemical results proved the 
      secretion of H+ and HCO(3)(-) ions, typical of digestive process. Colocalization 
      studies indicated a co-expression of the studied proteins in all considered 
      organs, excluding NHE and pendrin for renal papillae, since some renal papillae 
      are NHE immunopositive only.
CI  - Copyright (c) 2017 Elsevier GmbH. All rights reserved.
FAU - Cuoghi, Ivan
AU  - Cuoghi I
AD  - Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 
      213/D, I-41125 Modena, Italy.
FAU - Lazzaretti, Clara
AU  - Lazzaretti C
AD  - Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 
      213/D, I-41125 Modena, Italy.
FAU - Mandrioli, Mauro
AU  - Mandrioli M
AD  - Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 
      213/D, I-41125 Modena, Italy.
FAU - Mola, Lucrezia
AU  - Mola L
AD  - Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 
      213/D, I-41125 Modena, Italy. Electronic address: lucrezia.mola@unimore.it.
FAU - Pederzoli, Aurora
AU  - Pederzoli A
AD  - Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 
      213/D, I-41125 Modena, Italy.
LA  - eng
PT  - Journal Article
DEP - 20171121
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Protons)
RN  - 0 (Sodium-Hydrogen Exchangers)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Animals
MH  - Carbonic Anhydrases/chemistry
MH  - Computational Biology
MH  - Evolution, Molecular
MH  - *Immunohistochemistry
MH  - Lancelets/*chemistry
MH  - *Models, Biological
MH  - Phylogeny
MH  - Protons
MH  - Sodium-Hydrogen Exchangers/*chemistry
OTO - NOTNLM
OT  - Carbonic anhydrase II
OT  - Cystic fibrosis transmembrane regulator
OT  - Na(+)/H(+) exchanger
OT  - Na(+)/K(+) ATPase
OT  - Pendrin
OT  - Vacuolar-type H(+)-ATPase
EDAT- 2017/11/25 06:00
MHDA- 2018/11/28 06:00
CRDT- 2017/11/25 06:00
PHST- 2017/06/26 00:00 [received]
PHST- 2017/09/20 00:00 [revised]
PHST- 2017/10/30 00:00 [accepted]
PHST- 2017/11/25 06:00 [pubmed]
PHST- 2018/11/28 06:00 [medline]
PHST- 2017/11/25 06:00 [entrez]
AID - S0065-1281(17)30206-4 [pii]
AID - 10.1016/j.acthis.2017.10.011 [doi]
PST - ppublish
SO  - Acta Histochem. 2018 Jan;120(1):33-40. doi: 10.1016/j.acthis.2017.10.011. Epub 
      2017 Nov 21.