PMID- 29172213
OWN - NLM
STAT- MEDLINE
DCOM- 20180810
LR  - 20180810
IS  - 1098-9064 (Electronic)
IS  - 0094-6176 (Linking)
VI  - 44
IP  - 1
DP  - 2018 Feb
TI  - In Situ Endothelialization Promoted by SEMA4D and CXCL12 for Titanium-Based 
      Biomaterials.
PG  - 70-80
LID - 10.1055/s-0037-1605569 [doi]
AB  - In situ endothelialization, aiming to create implantation surfaces capable of 
      self-endothelialization, seems to be an extremely promising solution, 
      particularly on those blood-contacting surfaces. In this research study, we 
      immobilized the soluble semaphorin 4D (SEMA4D) and C-X-C motif chemokine ligand 
      12 (CXCL12) biomolecules together with heparin onto the metal-based biomaterial 
      surfaces to achieve in situ endothelialization of modified samples both by 
      stimulating the neighboring endothelial cells (ECs) migration and by capturing 
      the circulating endothelial progenitor cells (EPCs) directly from the blood 
      circulation. X-ray photoelectron spectroscopy data demonstrate the successful 
      immobilization of SEMA4D and CXCL12. Due to the presence of heparin, 
      hemocompatibility was also improved after modification. Although EC migration was 
      mainly mediated by SEMA4D with the coordination of CXCL12, EC proliferation and 
      haptotaxis property were also enhanced, while EC chemotaxis was slightly 
      suppressed because the further immobilization of CXCL12 influences the release of 
      SEMA4D. The results of the ex vivo EPC capturing assay indicated the mobilization 
      of CXCL12 promotes EPC adhesion. In vivo implantation further demonstrated that 
      CXCL12 cooperates with SEMA4D to promote a process of in situ endothelialization.
CI  - Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.
FAU - Cui, Yuanyuan
AU  - Cui Y
AD  - Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong 
      University, Chengdu, People's Republic of China.
FAU - Zhou, Feng
AU  - Zhou F
AD  - Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong 
      University, Chengdu, People's Republic of China.
AD  - Institute of Aeronautics and Astronautics, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Wei, Lai
AU  - Wei L
AD  - Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong 
      University, Chengdu, People's Republic of China.
FAU - Song, Qiang
AU  - Song Q
AD  - Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong 
      University, Chengdu, People's Republic of China.
FAU - Tan, Jianying
AU  - Tan J
AD  - Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong 
      University, Chengdu, People's Republic of China.
FAU - Zeng, Zheng
AU  - Zeng Z
AD  - Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong 
      University, Chengdu, People's Republic of China.
FAU - Chen, Junying
AU  - Chen J
AD  - Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong 
      University, Chengdu, People's Republic of China.
FAU - Huang, Nan
AU  - Huang N
AD  - Key Laboratory of Advanced Technology of Materials, Southwest Jiaotong 
      University, Chengdu, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20171124
PL  - United States
TA  - Semin Thromb Hemost
JT  - Seminars in thrombosis and hemostasis
JID - 0431155
RN  - 0 (Biocompatible Materials)
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Biocompatible Materials
MH  - Chemokine CXCL12/*metabolism
MH  - Endothelium, Vascular/*physiology
MH  - Humans
MH  - Titanium/*metabolism
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2017/11/25 06:00
MHDA- 2018/08/11 06:00
CRDT- 2017/11/25 06:00
PHST- 2017/11/25 06:00 [pubmed]
PHST- 2018/08/11 06:00 [medline]
PHST- 2017/11/25 06:00 [entrez]
AID - 10.1055/s-0037-1605569 [doi]
PST - ppublish
SO  - Semin Thromb Hemost. 2018 Feb;44(1):70-80. doi: 10.1055/s-0037-1605569. Epub 2017 
      Nov 24.