PMID- 29172449
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 19
IP  - 1
DP  - 2018 Jan 8
TI  - EXOPLEXs: Chimeric Drug Delivery Platform from the Fusion of Cell-Derived 
      Nanovesicles and Liposomes.
PG  - 22-30
LID - 10.1021/acs.biomac.7b01176 [doi]
AB  - Cell-derived nanovesicles (CDNs) have been recently investigated as novel drug 
      delivery systems (DDSs), due to the preservation of key features from the cell 
      membrane of their precursor cells, which are responsible for an efficient 
      cellular uptake by target cells. However, CDNs suffer from low drug loading 
      efficiencies as well as challenges in functionalization compared to conventional 
      DDS like liposomes. Here, we describe the first study proposing the fusion of 
      CDNs with liposomes to form EXOPLEXs. We report the preservation of cell 
      membranes from precursor cells similarly to CDNs, as well as high loading 
      efficiencies of more than 65% with doxorubicin hydrochloride, a model 
      chemotherapeutic drug. The doxorubicin-loaded EXOPLEXs (DOX-EXO) also 
      demonstrated a higher in vitro cell killing effect than liposomes, while EXOPLEXs 
      alone did not show any remarkable cytotoxicity. Taken together, these results 
      illustrate the potential of EXOPLEXs as a novel DDS for targeted delivery of 
      chemotherapeutics.
FAU - Goh, Wei Jiang
AU  - Goh WJ
AD  - NUS Graduate School for Integrative Sciences and Engineering, Centre for Life 
      Sciences (CeLS) , 28 Medical Drive, #05-01, Singapore 117456.
AD  - Department of Pharmacy, National University of Singapore , Science Drive 2, 
      S15#05, Singapore 117543.
FAU - Zou, Shui
AU  - Zou S
AD  - Department of Pharmacy, National University of Singapore , Science Drive 2, 
      S15#05, Singapore 117543.
FAU - Lee, Choon Keong
AU  - Lee CK
AD  - Department of Pharmacy, National University of Singapore , Science Drive 2, 
      S15#05, Singapore 117543.
FAU - Ou, Yi-Hsuan
AU  - Ou YH
AD  - Department of Pharmacy, National University of Singapore , Science Drive 2, 
      S15#05, Singapore 117543.
FAU - Wang, Jiong-Wei
AU  - Wang JW
AD  - Department of Surgery, Yong Loo Lin School of Medicine, National University of 
      Singapore , 1E Kent Ridge Road, Singapore 119228.
AD  - Cardiovascular Research Institute (CVRI), National University Heart Centre 
      Singapore (NUHCS) and National University Health System (NUHS) , Kent Ridge Road, 
      Singapore 119074.
FAU - Czarny, Bertrand
AU  - Czarny B
AD  - Department of Pharmacy, National University of Singapore , Science Drive 2, 
      S15#05, Singapore 117543.
FAU - Pastorin, Giorgia
AU  - Pastorin G
AUID- ORCID: 0000-0002-2568-0626
AD  - NUS Graduate School for Integrative Sciences and Engineering, Centre for Life 
      Sciences (CeLS) , 28 Medical Drive, #05-01, Singapore 117456.
AD  - Department of Pharmacy, National University of Singapore , Science Drive 2, 
      S15#05, Singapore 117543.
AD  - NUSNNI-NanoCore, National University of Singapore , T-Lab Level 11, 5A 
      Engineering Drive 1, Singapore 117580.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171208
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Liposomes)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Antibiotics, Antineoplastic/administration & dosage/pharmacology
MH  - *Cell Fusion
MH  - Cell Membrane/drug effects
MH  - *Cell-Derived Microparticles
MH  - Doxorubicin/administration & dosage/pharmacology
MH  - *Drug Delivery Systems
MH  - HeLa Cells
MH  - Humans
MH  - *Liposomes
MH  - *Nanostructures
MH  - U937 Cells
EDAT- 2017/11/28 06:00
MHDA- 2018/12/12 06:00
CRDT- 2017/11/28 06:00
PHST- 2017/11/28 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2017/11/28 06:00 [entrez]
AID - 10.1021/acs.biomac.7b01176 [doi]
PST - ppublish
SO  - Biomacromolecules. 2018 Jan 8;19(1):22-30. doi: 10.1021/acs.biomac.7b01176. Epub 
      2017 Dec 8.