PMID- 29173480
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR  - 20180709
IS  - 1878-3252 (Electronic)
IS  - 0946-672X (Linking)
VI  - 45
DP  - 2018 Jan
TI  - Changed expressions of N-methyl-d-aspartate receptors in the brains of rats and 
      primary neurons exposed to high level of fluoride.
PG  - 31-40
LID - S0946-672X(17)30702-2 [pii]
LID - 10.1016/j.jtemb.2017.09.020 [doi]
AB  - Expressions of N-methyl-d-aspartic acid receptors (NMDARs) in the brains of rats 
      and primary neurons exposed to high fluoride were investigated. Sprague-Dawley 
      rats were divided randomly into a fluorosis group (50ppm fluoride in the drinking 
      water for 6 months) and controls (<0.5ppm fluoride) and the offspring from these 
      rats sacrificed on postnatal days 1, 7, 14, 21 and 28. The primary cultured 
      neurons from the hippocampus of neonatal rats were treated with 5 and 50ppm 
      fluoride for 48h. NMDAR subunits at protein or mRNA levels were quantified by 
      Western blotting or real-time PCR. The phosphorylated calmodulin-protein kinase 
      II (CaMKII) was determined by Western blotting, concentration of Ca(2+) in 
      neurons by laser confocal microscopy and apoptosis by flow cytometry. In the 
      brains of adult rats and pups as well as in primary neurons exposed to high 
      fluoride, the mRNAs encoding GluN1 and GluN2B subunits and the corresponding 
      proteins were elevated, the GluN3A lowered and the GluN2A unchanged. In addition, 
      the level of phosphor-CaMKII was reduced, and Ca(2+) influx and apoptosis 
      enhanced in the brains of rats and cultured neurons exposed to high fluoride. The 
      results indicate that such modifications may involve brain damage induced by 
      chronic fluorosis.
CI  - Copyright (c) 2017 Elsevier GmbH. All rights reserved.
FAU - Wei, Na
AU  - Wei N
AD  - Department of Pathology in the Affiliated Hospital of Guizhou Medical University, 
      PR China.
FAU - Dong, Yang-Ting
AU  - Dong YT
AD  - Department of Pathology in the Affiliated Hospital of Guizhou Medical University, 
      PR China.
FAU - Deng, Jie
AU  - Deng J
AD  - Department of Pathology in the Affiliated Hospital of Guizhou Medical University, 
      PR China; Key Laboratory of Medical Molecular Biology (Guizhou Medical 
      University), Guizhou Province, PR China.
FAU - Wang, Ya
AU  - Wang Y
AD  - Department of Pathology in the Affiliated Hospital of Guizhou Medical University, 
      PR China.
FAU - Qi, Xiao-Lan
AU  - Qi XL
AD  - Key Laboratory of Endemic and Ethnic Diseases (Guizhou Medical University), 
      Ministry of Education, PR China; Key Laboratory of Medical Molecular Biology 
      (Guizhou Medical University), Guizhou Province, PR China.
FAU - Yu, Wen-Feng
AU  - Yu WF
AD  - Key Laboratory of Endemic and Ethnic Diseases (Guizhou Medical University), 
      Ministry of Education, PR China; Key Laboratory of Medical Molecular Biology 
      (Guizhou Medical University), Guizhou Province, PR China.
FAU - Xiao, Yan
AU  - Xiao Y
AD  - Key Laboratory of Endemic and Ethnic Diseases (Guizhou Medical University), 
      Ministry of Education, PR China; Key Laboratory of Medical Molecular Biology 
      (Guizhou Medical University), Guizhou Province, PR China.
FAU - Zhou, Jian-Jiang
AU  - Zhou JJ
AD  - Key Laboratory of Endemic and Ethnic Diseases (Guizhou Medical University), 
      Ministry of Education, PR China; Key Laboratory of Medical Molecular Biology 
      (Guizhou Medical University), Guizhou Province, PR China.
FAU - Guan, Zhi-Zhong
AU  - Guan ZZ
AD  - Department of Pathology in the Affiliated Hospital of Guizhou Medical University, 
      PR China; Key Laboratory of Endemic and Ethnic Diseases (Guizhou Medical 
      University), Ministry of Education, PR China; Key Laboratory of Medical Molecular 
      Biology (Guizhou Medical University), Guizhou Province, PR China. Electronic 
      address: 1457658298@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20170921
PL  - Germany
TA  - J Trace Elem Med Biol
JT  - Journal of trace elements in medicine and biology : organ of the Society for 
      Minerals and Trace Elements (GMS)
JID - 9508274
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - Q80VPU408O (Fluorides)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Brain/*metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
MH  - Cells, Cultured
MH  - Flow Cytometry
MH  - Fluorides/*pharmacology
MH  - Neurons/*drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/genetics/*metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Ca MKIIalpha
OT  - Ca(2+) influx
OT  - Chronic fluorosis
OT  - NMDARs
OT  - Rat brains
EDAT- 2017/11/28 06:00
MHDA- 2018/07/10 06:00
CRDT- 2017/11/28 06:00
PHST- 2017/07/20 00:00 [received]
PHST- 2017/09/15 00:00 [revised]
PHST- 2017/09/19 00:00 [accepted]
PHST- 2017/11/28 06:00 [entrez]
PHST- 2017/11/28 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
AID - S0946-672X(17)30702-2 [pii]
AID - 10.1016/j.jtemb.2017.09.020 [doi]
PST - ppublish
SO  - J Trace Elem Med Biol. 2018 Jan;45:31-40. doi: 10.1016/j.jtemb.2017.09.020. Epub 
      2017 Sep 21.