PMID- 29174314
OWN - NLM
STAT- MEDLINE
DCOM- 20180731
LR  - 20180731
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 36
IP  - 1
DP  - 2018 Jan 2
TI  - Development of a broadly protective modified-live virus vaccine candidate against 
      porcine reproductive and respiratory syndrome virus.
PG  - 66-73
LID - S0264-410X(17)31592-X [pii]
LID - 10.1016/j.vaccine.2017.11.028 [doi]
AB  - Modified-live virus (MLV) vaccines are widely used to protect pigs against 
      porcine reproductive and respiratory syndrome virus (PRRSV). However, current MLV 
      vaccines do not confer adequate levels of heterologous protection, presumably due 
      to the substantial genetic diversity of PRRSV isolates circulating in the field. 
      To overcome this genetic variation challenge, we recently generated a synthetic 
      PRRSV strain containing a consensus genomic sequence of PRRSV-2. We demonstrated 
      that our synthetic PRRSV strain confers unprecedented levels of heterologous 
      protection. However, the synthetic PRRSV strain at passage 1 (hereafter 
      designated CON-P1) is highly virulent and therefore, is not suitable to be used 
      as a vaccine in pigs. In the present study, we attenuated CON-P1 by continuously 
      passaging the virus in MARC-145 cells, a non-natural host cell line. Using a 
      young pig model, we demonstrated that the synthetic virus at passages 90 and 122 
      (designated as CON-P90 and CON-P122, respectively) were fully attenuated, as 
      evidenced by the significantly reduced viral loads in serum and tissues and the 
      absence of lung lesion in the infected pigs. Most importantly, CON-P90 confers 
      similar levels of heterologous protection as its parental strain CON-P1. Taken 
      together, the results indicate that CON-P90 is an excellent candidate for the 
      formulation of next generation of PRRSV MLV vaccines with improved levels of 
      heterologous protection.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Sun, Haiyan
AU  - Sun H
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, USA; 
      School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE, USA.
FAU - Workman, Aspen
AU  - Workman A
AD  - USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA.
FAU - Osorio, Fernando A
AU  - Osorio FA
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, USA; 
      School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE, USA.
FAU - Steffen, David
AU  - Steffen D
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, NE, USA.
FAU - Vu, Hiep L X
AU  - Vu HLX
AD  - Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, USA; 
      Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE, USA. 
      Electronic address: hiepvu@unl.edu.
LA  - eng
PT  - Journal Article
DEP - 20171122
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Antibodies, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Cell Line
MH  - Immunity, Heterologous/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage/*immunology
MH  - Viral Load
MH  - Viral Vaccines/administration & dosage/genetics/*immunology
MH  - Viremia/immunology/prevention & control
MH  - Virology/methods
OTO - NOTNLM
OT  - Heterologous protection
OT  - MLV
OT  - PRRSV
OT  - Synthetic virus
EDAT- 2017/11/28 06:00
MHDA- 2018/08/01 06:00
CRDT- 2017/11/28 06:00
PHST- 2017/08/08 00:00 [received]
PHST- 2017/10/23 00:00 [revised]
PHST- 2017/11/13 00:00 [accepted]
PHST- 2017/11/28 06:00 [pubmed]
PHST- 2018/08/01 06:00 [medline]
PHST- 2017/11/28 06:00 [entrez]
AID - S0264-410X(17)31592-X [pii]
AID - 10.1016/j.vaccine.2017.11.028 [doi]
PST - ppublish
SO  - Vaccine. 2018 Jan 2;36(1):66-73. doi: 10.1016/j.vaccine.2017.11.028. Epub 2017 
      Nov 22.