PMID- 29175695
OWN - NLM
STAT- MEDLINE
DCOM- 20180223
LR  - 20181202
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1072
DP  - 2018 Jan 1
TI  - Determination of non-liposomal and liposomal doxorubicin in plasma by LC-MS/MS 
      coupled with an effective solid phase extraction: In comparison with 
      ultrafiltration technique and application to a pharmacokinetic study.
PG  - 149-160
LID - S1570-0232(17)31151-0 [pii]
LID - 10.1016/j.jchromb.2017.11.020 [doi]
AB  - Liposomal formulation of doxorubicin has been widely applied in clinic for 
      treatment of various cancers. The separation and measurement of free drug (drug 
      which is not entrapped in liposomes) and liposomal drug in the plasma after 
      injection of liposomal doxorubicin is of prime importance due to toxicity and 
      activity concerns. In this study, a rapid and convenient method was developed to 
      isolate and determine the non-liposomal and liposomal drugs in plasma. Plasma 
      samples were prepared by solid phase extraction (SPE) using Oasis HLB cartridges. 
      Liposomal doxorubicin (L-DOX) was collected in the aqueous eluate with its 
      internal standard (IS), metformin; and non-liposomal doxorubicin (NL-DOX) and its 
      isotope labelling IS were eluted from the cartridge by methanol containing 0.5% 
      formic acid. After SPE separation, L-DOX and NL-DOX were subsequently quantified 
      by a validated sensitive LC-MS/MS method individually. The calibration curves 
      were found to be linear for L-DOX in the range of 0.156-40.0mug/mL and for NL-DOX 
      in the range of 3.13-200ng/mL. The extraction recovery was about 97% for L-DOX 
      and about 65% for NL-DOX. This method was further applied to investigate the 
      pharmacokinetics of doxorubicin in Beagle dogs after an intravenous dose of 
      1.0mg/kg Doxil((R)). After injection of Doxil((R)), L-DOX was the predominant 
      component circulating in plasma, whose amount was about 1000-fold higher than 
      that of NL-DOX. The analytical method might be helpful in pharmacokinetics and 
      toxicity assessment of liposomal formulation.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Xie, Yaping
AU  - Xie Y
AD  - Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Shao, Nan
AU  - Shao N
AD  - Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Jin, Yi
AU  - Jin Y
AD  - Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Zhang, Liang
AU  - Zhang L
AD  - Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Jiang, Huan
AU  - Jiang H
AD  - Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Xiong, Ningjie
AU  - Xiong N
AD  - Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Su, Fangming
AU  - Su F
AD  - Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China.
FAU - Xu, Haiyan
AU  - Xu H
AD  - Pharmacy School, Shenyang Pharmaceutical University, Shenyang 110016, China. 
      Electronic address: xhy1020@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20171116
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (liposomal doxorubicin)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Dogs
MH  - Doxorubicin/*analogs & derivatives/blood/chemistry/pharmacokinetics
MH  - Female
MH  - Humans
MH  - Limit of Detection
MH  - Linear Models
MH  - Male
MH  - Polyethylene Glycols/chemistry/pharmacokinetics
MH  - Reproducibility of Results
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
MH  - Ultrafiltration
OTO - NOTNLM
OT  - Doxorubicin
OT  - LC-MS/MS
OT  - Liposome
OT  - Solid phase extraction
EDAT- 2017/11/28 06:00
MHDA- 2018/02/24 06:00
CRDT- 2017/11/28 06:00
PHST- 2017/07/04 00:00 [received]
PHST- 2017/10/26 00:00 [revised]
PHST- 2017/11/16 00:00 [accepted]
PHST- 2017/11/28 06:00 [pubmed]
PHST- 2018/02/24 06:00 [medline]
PHST- 2017/11/28 06:00 [entrez]
AID - S1570-0232(17)31151-0 [pii]
AID - 10.1016/j.jchromb.2017.11.020 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 1;1072:149-160. doi: 
      10.1016/j.jchromb.2017.11.020. Epub 2017 Nov 16.