PMID- 29176662
OWN - NLM
STAT- MEDLINE
DCOM- 20180828
LR  - 20181127
IS  - 2044-5385 (Electronic)
IS  - 2044-5385 (Linking)
VI  - 7
IP  - 12
DP  - 2017 Nov 27
TI  - Minimal residual disease by either flow cytometry or cytogenetics prior to an 
      allogeneic hematopoietic stem cell transplant is associated with poor outcome in 
      acute myeloid leukemia.
PG  - 634
LID - 10.1038/s41408-017-0007-x [doi]
LID - 634
AB  - Relapsed acute myeloid leukemia (AML) is a significant challenge after allogeneic 
      hematopoietic cell transplant (HCT). Multiparameter flow cytometry (MFC), 
      conventional cytogenetics (CG), and fluorescence in situ hybridization (FISH) are 
      routinely performed on bone marrow specimens prior to HCT to assess disease 
      status. We questioned the extent by which pre-HCT evidence of minimal residual 
      disease (MRD) detected by these standard assays, corresponded with post-HCT 
      relapse. We conducted a single center, retrospective study of 166 AML patients 
      who underwent HCT. Thirty-eight of one hundred sixty-six (23%) patients in 
      complete remission (CR) or CR with incomplete count recovery (CRi) had MRD 
      detectable by MFC, CG, or FISH. MRD was more frequently seen in patients with 
      poor risk karyotype at diagnosis (P = 0.011). MRD-negative patients (MRD(neg)) 
      had significantly longer overall survival (OS) and relapse-free survival than 
      patients who were MRD positive (MRD(pos)) (P = 0.002 and 0.013, respectively). In 
      patients with MRD(pos) prior to HCT, the presence of acute graft vs. host disease 
      (GVHD) (grade >/= 2) or chronic GVHD significantly improved progression free 
      survival (PFS) (hazard ratio (HR) = 0.053 (95% confidence interval (CI): 
      0.01-0.279), P = 0.0005) and OS (HR = 0.211 (95% CI: 0.081-0.547), P = 0.0014).
FAU - Norkin, Maxim
AU  - Norkin M
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA. norkinm@ufl.edu.
FAU - Katragadda, Lakshmikanth
AU  - Katragadda L
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Zou, Fei
AU  - Zou F
AD  - Department of Biostatistics, College of Medicine, University of Florida, 
      Gainesville, FL, USA.
FAU - Xiong, Sican
AU  - Xiong S
AD  - Department of Biostatistics, College of Medicine, University of Florida, 
      Gainesville, FL, USA.
FAU - Chang, Myron
AU  - Chang M
AD  - Department of Biostatistics, College of Medicine, University of Florida, 
      Gainesville, FL, USA.
FAU - Dai, Yunfeng
AU  - Dai Y
AD  - Department of Biostatistics, College of Medicine, University of Florida, 
      Gainesville, FL, USA.
FAU - Hsu, Jack W
AU  - Hsu JW
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Moreb, Jan S
AU  - Moreb JS
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Leather, Helen
AU  - Leather H
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Murthy, Hemant S
AU  - Murthy HS
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Farhadfar, Nosha
AU  - Farhadfar N
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Li, Ying
AU  - Li Y
AD  - Department of Pathology, College of Medicine, University of Florida, Gainesville, 
      FL, USA.
FAU - Hromas, Robert
AU  - Hromas R
AUID- ORCID: 0000-0002-1916-6276
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Brown, Randy A
AU  - Brown RA
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Cogle, Christopher R
AU  - Cogle CR
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
FAU - Wingard, John R
AU  - Wingard JR
AD  - Division of Hematology and Oncology, Department of Internal Medicine, College of 
      Medicine, University of Florida, Gainesville, FL, USA.
LA  - eng
PT  - Journal Article
DEP - 20171127
PL  - United States
TA  - Blood Cancer J
JT  - Blood cancer journal
JID - 101568469
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cytogenetic Analysis
MH  - Disease-Free Survival
MH  - Female
MH  - Flow Cytometry
MH  - Graft vs Host Disease
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Leukemia, Myeloid, Acute/mortality/*pathology/*surgery
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/mortality/pathology/surgery
MH  - Neoplasm, Residual/mortality/*pathology/*surgery
MH  - Prognosis
MH  - Proportional Hazards Models
MH  - Retrospective Studies
MH  - Transplantation, Homologous
MH  - Treatment Outcome
PMC - PMC5802525
COIS- COMPETING INTERESTS: The authors declare that they have no competing interests. 
      PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional 
      claims in published maps and institutional affiliations.
EDAT- 2017/11/28 06:00
MHDA- 2018/08/29 06:00
PMCR- 2017/11/27
CRDT- 2017/11/28 06:00
PHST- 2017/08/20 00:00 [received]
PHST- 2017/08/25 00:00 [accepted]
PHST- 2017/11/28 06:00 [entrez]
PHST- 2017/11/28 06:00 [pubmed]
PHST- 2018/08/29 06:00 [medline]
PHST- 2017/11/27 00:00 [pmc-release]
AID - 10.1038/s41408-017-0007-x [pii]
AID - 7 [pii]
AID - 10.1038/s41408-017-0007-x [doi]
PST - epublish
SO  - Blood Cancer J. 2017 Nov 27;7(12):634. doi: 10.1038/s41408-017-0007-x.