PMID- 29177857
OWN - NLM
STAT- MEDLINE
DCOM- 20180719
LR  - 20210702
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 1714
DP  - 2018
TI  - Biochemical Isolation of the Myddosome from Murine Macrophages.
PG  - 79-95
LID - 10.1007/978-1-4939-7519-8_6 [doi]
AB  - Ligand-induced macromolecular protein complex formation has emerged as a common 
      means by which the innate immune system activates signal transduction pathways 
      essential for host defense. Despite their structural divergence, key signaling 
      molecules in diverse innate immune pathways mediate signal transduction by 
      assembling higher-order protein complexes at specific subcellular locations in a 
      stimulus-dependent manner. These protein complexes are collectively known as the 
      supramolecular organizing centers (SMOCs), which link active receptors to a 
      variety of downstream cellular responses. In the Toll-like receptor (TLR) 
      pathway, the signaling adaptor MyD88 is the core of a SMOC called the myddosome, 
      which is composed of the sorting adaptor TIRAP and the IRAK family kinases. 
      Depending on the microbial ligands encountered, the myddosome can be assembled at 
      the plasma membrane or endosomes, thereby leading to NF-kB and AP-1 activation, 
      and the subsequent expression of pro-inflammatory cytokines. Herein, we provide a 
      detailed protocol for studying myddosome assembly in murine bone marrow-derived 
      macrophages (BMDMs).
FAU - Tan, Yunhao
AU  - Tan Y
AD  - Division of Gastroenterology, Harvard Medical School, Boston Children's Hospital, 
      Boston, MA, USA.
FAU - Kagan, Jonathan C
AU  - Kagan JC
AD  - Division of Gastroenterology, Harvard Medical School, Boston Children's Hospital, 
      Boston, MA, USA. jonathan.kagan@childrens.harvard.edu.
LA  - eng
GR  - P30 DK034854/DK/NIDDK NIH HHS/United States
GR  - R01 AI093589/AI/NIAID NIH HHS/United States
GR  - R01 AI116550/AI/NIAID NIH HHS/United States
GR  - R37 AI116550/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Interleukin-1)
RN  - 0 (TIRAP protein, mouse)
RN  - EC 2.7.11.1 (Interleukin-1 Receptor-Associated Kinases)
RN  - EC 2.7.11.1 (Irak4 protein, mouse)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Immunoprecipitation/*methods
MH  - Interleukin-1 Receptor-Associated Kinases/isolation & purification/metabolism
MH  - Macrophages/cytology/*metabolism
MH  - Membrane Glycoproteins/isolation & purification/metabolism
MH  - Mice
MH  - Multiprotein Complexes/*isolation & purification/metabolism
MH  - Myeloid Differentiation Factor 88/*isolation & purification/metabolism
MH  - Receptors, Interleukin-1/isolation & purification/metabolism
PMC - PMC6698367
MID - NIHMS1045998
OTO - NOTNLM
OT  - Immunoprecipitation
OT  - MyD88
OT  - Myddosome
OT  - SMOCs
OT  - TLR
EDAT- 2017/11/28 06:00
MHDA- 2018/07/20 06:00
PMCR- 2019/08/18
CRDT- 2017/11/28 06:00
PHST- 2017/11/28 06:00 [entrez]
PHST- 2017/11/28 06:00 [pubmed]
PHST- 2018/07/20 06:00 [medline]
PHST- 2019/08/18 00:00 [pmc-release]
AID - 10.1007/978-1-4939-7519-8_6 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1714:79-95. doi: 10.1007/978-1-4939-7519-8_6.