PMID- 29178679
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2092-7355 (Print)
IS  - 2092-7363 (Electronic)
IS  - 2092-7355 (Linking)
VI  - 10
IP  - 1
DP  - 2018 Jan
TI  - Detecting Allergens From Black Tiger Shrimp Penaeus monodon That Can Bind and 
      Cross-link IgE by ELISA, Western Blot, and a Humanized Rat Basophilic Leukemia 
      Reporter Cell Line RS-ATL8.
PG  - 62-76
LID - 10.4168/aair.2018.10.1.62 [doi]
AB  - BACKGROUND: Black tiger shrimp Penaeus monodon is one of the common causes of 
      shellfish allergy that is increasing worldwide. One of the important problems in 
      the management of shellfish allergy is the lack of accurate diagnostic assay 
      because the biological and immunological properties of allergens in black tiger 
      shrimp have not been well characterized. This study aims to detect proteins with 
      the ability to bind and cross-link immunoglobulin E (IgE) from black tiger shrimp 
      by enzyme-linked immunosorbent assay (ELISA), Western blot, and a humanized rat 
      basophilic leukemia reporter cell line RS-ATL8. METHODS: Sera from shrimp 
      allergic subjects were subjected to ELISA and Western blots using raw or cooked 
      shrimp extract as antigens. Pooled sera were used to sensitize the RS-ATL8 
      reporter cell line and cells were activated by shrimp extract. Eluted protein 
      extracts separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis 
      (SDS-PAGE) were tested on the RS-ATL8 cell line and subjected to mass 
      spectrometry to identify potential candidate allergens. RESULTS: Allergic sera 
      reacted stronger to raw shrimp extract than cooked shrimp extract (P=0.009). 
      Western blot demonstrated that major IgE reactivity protein bands were at 32-39 
      kDa and 91-230 kDa in both raw and cooked shrimp extracts. The eluted protein 
      bands at the molecular weight of 38 and 115 kDa from raw shrimp extract induced 
      IgE cross-linking as assayed by the RS-ATL8 cell line. These protein bands were 
      subjected to mass spectrometry for analysis. Ubiquitin-activating enzyme and 
      crustacyanin were identified as potential candidate novel shrimp allergens. 
      CONCLUSIONS: The RS-ATL8 reporter cell line can be used to identify potential new 
      shrimp allergens that can functionally cross-link IgE and induce mast cell 
      degranulation.
CI  - Copyright (c) 2018 The Korean Academy of Asthma, Allergy and Clinical Immunology . 
      The Korean Academy of Pediatric Allergy and Respiratory Disease
FAU - Jarupalee, Thanyapat
AU  - Jarupalee T
AD  - Graduate Program in Biotechnology, Faculty of Science, Chulalongkorn University, 
      Bangkok, Thailand.
AD  - Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.
FAU - Chatchatee, Pantipa
AU  - Chatchatee P
AD  - Allergy&Immunology Unit, Department of Pediatrics, Faculty of Medicine, 
      Chulalongkorn University, Bangkok, Thailand.
FAU - Komolpis, Kittinan
AU  - Komolpis K
AD  - Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 
      Bangkok, Thailand.
FAU - Suratannon, Narissara
AU  - Suratannon N
AD  - Allergy&Immunology Unit, Department of Pediatrics, Faculty of Medicine, 
      Chulalongkorn University, Bangkok, Thailand.
FAU - Roytrakul, Sittiruk
AU  - Roytrakul S
AD  - National Center for Genetic Engineering and Biotechnology (BIOTEC), National 
      Science and Technology Development Agency, Pathum Thani, Thailand.
FAU - Yingchutrakul, Yodying
AU  - Yingchutrakul Y
AD  - National Center for Genetic Engineering and Biotechnology (BIOTEC), National 
      Science and Technology Development Agency, Pathum Thani, Thailand.
FAU - Yimchuen, Wanaporn
AU  - Yimchuen W
AD  - Department of Microbiology, Faculty of Science, Chulalongkorn University, 
      Bangkok, Thailand.
FAU - Butta, Patcharavadee
AU  - Butta P
AD  - Department of Microbiology, Faculty of Science, Chulalongkorn University, 
      Bangkok, Thailand.
FAU - Jacquet, Alain
AU  - Jacquet A
AD  - Chula-Vaccine Research Center, Faculty of Medicine, Chulalongkorn University, 
      Bangkok, Thailand.
FAU - Palaga, Tanapat
AU  - Palaga T
AD  - Department of Microbiology, Faculty of Science, Chulalongkorn University, 
      Bangkok, Thailand.
AD  - Center of Excellence in Immunology and Immune-mediated Diseases, Chulalongkorn 
      University, Bangkok, Thailand. tanapat.p@chula.ac.th.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Allergy Asthma Immunol Res
JT  - Allergy, asthma & immunology research
JID - 101518382
PMC - PMC5705486
OTO - NOTNLM
OT  - IgE
OT  - Penaeus monodon
OT  - allergens
OT  - humanized basophilic leukaemia reporter cell
COIS- There are no financial or other issues that might lead to conflict of interest.
EDAT- 2017/11/28 06:00
MHDA- 2017/11/28 06:01
PMCR- 2018/01/01
CRDT- 2017/11/28 06:00
PHST- 2017/01/19 00:00 [received]
PHST- 2017/08/01 00:00 [revised]
PHST- 2017/08/06 00:00 [accepted]
PHST- 2017/11/28 06:00 [entrez]
PHST- 2017/11/28 06:00 [pubmed]
PHST- 2017/11/28 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.62 [pii]
AID - 10.4168/aair.2018.10.1.62 [doi]
PST - ppublish
SO  - Allergy Asthma Immunol Res. 2018 Jan;10(1):62-76. doi: 10.4168/aair.2018.10.1.62.