PMID- 29180484
OWN - NLM
STAT- MEDLINE
DCOM- 20180627
LR  - 20211204
IS  - 2051-817X (Electronic)
IS  - 2051-817X (Linking)
VI  - 5
IP  - 22
DP  - 2017 Nov
TI  - Repeated bouts of resistance exercise with short recovery periods activates mTOR 
      signaling, but not protein synthesis, in mouse skeletal muscle.
LID - 10.14814/phy2.13515 [doi]
LID - e13515
AB  - The recovery period between bouts of exercise is one of the major factors 
      influencing the effects of resistance exercise, in addition to exercise intensity 
      and volume. However, the effects of shortening the recovery time between bouts of 
      resistance exercise on subsequent protein synthesis remain unclear. In this 
      study, we investigated the consequences of shortening the recovery time between 
      bouts of resistance exercise on protein synthesis and related processes in mouse 
      skeletal muscles. Eighteen male C57BL/6J mice were randomly subjected to three 
      bouts of resistance exercise with 72 (72H), 24 (24H), or 8 h (8H) of recovery 
      periods between bouts. Resistance exercise, consisting of five sets of 3 s x 10 
      isometric contractions with 3 min rest between sets, was elicited on the right 
      tibialis anterior muscle via percutaneous electrical stimulation on the deep 
      peroneal nerve under isoflurane anesthesia. The left muscle served as an internal 
      control. Six hours after the third bout of exercise, protein synthesis was found 
      to be activated in the 72H and 24H groups, but not in the 8H group. 
      Phosphorylation of p70S6K at Thr 389, a marker of mammalian target of rapamycin 
      (mTOR) signaling, was increased in all groups, with the 8H group showing the 
      highest magnitude. In contrast, protein carbonylation was observed only in mice 
      in the 8H group. These results suggest that repeated bouts of resistance exercise 
      with 8 h of recovery periods do not effectively increase the levels of muscle 
      protein synthesis despite activation of the mTOR signaling pathway, which likely 
      involves oxidative stress.
CI  - (c) 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on 
      behalf of The Physiological Society and the American Physiological Society.
FAU - Takegaki, Junya
AU  - Takegaki J
AD  - Department of Life Sciences, Graduate School of Arts and Sciences, The University 
      of Tokyo, Tokyo, Japan takegakij@gmail.com.
FAU - Ogasawara, Riki
AU  - Ogasawara R
AD  - Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, 
      Nagoya, Japan.
FAU - Tamura, Yuki
AU  - Tamura Y
AD  - Graduate School of Health and Sport Science, Nippon Sport Science University, 
      Tokyo, Japan.
FAU - Takagi, Ryo
AU  - Takagi R
AD  - Department of Life Sciences, Graduate School of Arts and Sciences, The University 
      of Tokyo, Tokyo, Japan.
FAU - Arihara, Yuki
AU  - Arihara Y
AD  - Department of Life Sciences, Graduate School of Arts and Sciences, The University 
      of Tokyo, Tokyo, Japan.
FAU - Tsutaki, Arata
AU  - Tsutaki A
AD  - Graduate School of Health and Sport Science, Nippon Sport Science University, 
      Tokyo, Japan.
FAU - Nakazato, Koichi
AU  - Nakazato K
AD  - Graduate School of Health and Sport Science, Nippon Sport Science University, 
      Tokyo, Japan.
FAU - Ishii, Naokata
AU  - Ishii N
AD  - Department of Life Sciences, Graduate School of Arts and Sciences, The University 
      of Tokyo, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Physiol Rep
JT  - Physiological reports
JID - 101607800
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Isometric Contraction
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muscle, Skeletal/*metabolism/physiology
MH  - Phosphorylation
MH  - *Physical Conditioning, Animal
MH  - Protein Carbonylation
MH  - Protein Processing, Post-Translational
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC5704086
OTO - NOTNLM
OT  - mTOR signaling
OT  - protein synthesis
OT  - recovery
OT  - resistance exercise
EDAT- 2017/11/29 06:00
MHDA- 2018/06/28 06:00
PMCR- 2017/11/28
CRDT- 2017/11/29 06:00
PHST- 2017/08/04 00:00 [received]
PHST- 2017/10/31 00:00 [revised]
PHST- 2017/11/01 00:00 [accepted]
PHST- 2017/11/29 06:00 [entrez]
PHST- 2017/11/29 06:00 [pubmed]
PHST- 2018/06/28 06:00 [medline]
PHST- 2017/11/28 00:00 [pmc-release]
AID - 5/22/e13515 [pii]
AID - PHY213515 [pii]
AID - 10.14814/phy2.13515 [doi]
PST - ppublish
SO  - Physiol Rep. 2017 Nov;5(22):e13515. doi: 10.14814/phy2.13515.