PMID- 2918068
OWN - NLM
STAT- MEDLINE
DCOM- 19890406
LR  - 20190510
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 4
IP  - 2
DP  - 1989 Feb
TI  - Recovery of motile sperm using the migration-sedimentation technique in an 
      in-vitro fertilization-embryo transfer programme.
PG  - 163-5
AB  - Sperm washing techniques, based on the swim-up principle used before inseminating 
      the human oocyte in in-vitro fertilization and embryo transfer programmes 
      (IVF-ET), usually require prior centrifugation which causes damage to the sperm 
      cell. A technique is described for separating sperm at laboratory temperature 
      based on sperm migration--sedimentation principles, using two concentric tubes 
      and recovering 70-90% forward-moving cells. A group of 17 patients is presented 
      who were managed with this method. The results were 85% fertilization rate, 4% 
      polyspermia and six clinical pregnancies.
FAU - Lucena, E
AU  - Lucena E
AD  - Centro Colombiano de Fertilidad y Esterilidad, Bogota.
FAU - Lucena, C
AU  - Lucena C
FAU - Gomez, M
AU  - Gomez M
FAU - Ortiz, J A
AU  - Ortiz JA
FAU - Ruiz, J
AU  - Ruiz J
FAU - Arango, A
AU  - Arango A
FAU - Diaz, C
AU  - Diaz C
FAU - Beuerman, C
AU  - Beuerman C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Cell Separation
MH  - Embryo Transfer/*methods
MH  - Female
MH  - Fertilization in Vitro/*methods
MH  - Humans
MH  - Male
MH  - Sperm Motility
MH  - Spermatozoa/*physiology
EDAT- 1989/02/01 00:00
MHDA- 1989/02/01 00:01
CRDT- 1989/02/01 00:00
PHST- 1989/02/01 00:00 [pubmed]
PHST- 1989/02/01 00:01 [medline]
PHST- 1989/02/01 00:00 [entrez]
AID - 10.1093/oxfordjournals.humrep.a136864 [doi]
PST - ppublish
SO  - Hum Reprod. 1989 Feb;4(2):163-5. doi: 10.1093/oxfordjournals.humrep.a136864.