PMID- 29182571
OWN - NLM
STAT- MEDLINE
DCOM- 20180709
LR  - 20240327
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 12
DP  - 2017 Nov 28
TI  - Graphene Oxide-Silver Nanoparticles Nanocomposite Stimulates Differentiation in 
      Human Neuroblastoma Cancer Cells (SH-SY5Y).
LID - 10.3390/ijms18122549 [doi]
LID - 2549
AB  - Recently, graphene and graphene related nanocomposite receive much attention due 
      to high surface-to-volume ratio, and unique physiochemical and biological 
      properties. The combination of metallic nanoparticles with graphene-based 
      materials offers a promising method to fabricate novel graphene-silver hybrid 
      nanomaterials with unique functions in biomedical nanotechnology, and 
      nanomedicine. Therefore, this study was designed to prepare graphene oxide (GO) 
      silver nanoparticles (AgNPs) nanocomposite (GO-AgNPs) containing two different 
      nanomaterials in single platform with distinctive properties using luciferin as 
      reducing agents. In addition, we investigated the effect of GO-AgNPs on 
      differentiation in SH-SY5Y cells. The synthesized GO-AgNPs were characterized by 
      ultraviolet-visible absorption spectroscopy (UV-vis), X-ray diffraction (XRD), 
      scanning electron microscopy (SEM), transmission electron microscopy (TEM) and 
      Raman spectroscopy. The differentiation was confirmed by series of cellular and 
      biochemical assays. The AgNPs were distributed uniformly on the surface of 
      graphene oxide with an average size of 25 nm. As prepared GO-AgNPOs induces 
      differentiation by increasing the expression of neuronal differentiation markers 
      and decreasing the expression of stem cell markers. The results indicated that 
      the redox biology involved the expression of various signaling molecules, which 
      play an important role in differentiation. This study suggests that GO-AgNP 
      nanocomposite could stimulate differentiation of SH-SY5Y cells. Furthermore, 
      understanding the mechanisms of differentiation of neuroblastoma cells could 
      provide new strategies for cancer and stem cell therapies. Therefore, these 
      studies suggest that GO-AgNPs could target specific chemotherapy-resistant cells 
      within a tumor.
FAU - Gurunathan, Sangiliyandi
AU  - Gurunathan S
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      05029, Korea. gsangiliyandi@yahoo.com.
FAU - Kim, Jin-Hoi
AU  - Kim JH
AUID- ORCID: 0000-0003-1232-5307
AD  - Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 
      05029, Korea. jhkim541@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20171128
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Reactive Oxygen Species)
RN  - 3M4G523W1G (Silver)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Differentiation/genetics/physiology
MH  - Cell Line, Tumor
MH  - Graphite/*chemistry
MH  - Humans
MH  - Metal Nanoparticles/*chemistry
MH  - Microscopy, Electron, Scanning
MH  - Nanocomposites/*chemistry
MH  - Neuroblastoma/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Silver/*chemistry
MH  - Spectrum Analysis, Raman
MH  - X-Ray Diffraction
PMC - PMC5751152
OTO - NOTNLM
OT  - apoptosis
OT  - differentiation
OT  - luciferin
OT  - neuroblastoma
OT  - neuronal markers
OT  - stem cell markers
COIS- The authors declare no conflict of interest.
EDAT- 2017/11/29 06:00
MHDA- 2018/07/10 06:00
PMCR- 2017/12/01
CRDT- 2017/11/29 06:00
PHST- 2017/10/26 00:00 [received]
PHST- 2017/11/17 00:00 [revised]
PHST- 2017/11/23 00:00 [accepted]
PHST- 2017/11/29 06:00 [entrez]
PHST- 2017/11/29 06:00 [pubmed]
PHST- 2018/07/10 06:00 [medline]
PHST- 2017/12/01 00:00 [pmc-release]
AID - ijms18122549 [pii]
AID - ijms-18-02549 [pii]
AID - 10.3390/ijms18122549 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Nov 28;18(12):2549. doi: 10.3390/ijms18122549.