PMID- 29185369
OWN - NLM
STAT- MEDLINE
DCOM- 20180903
LR  - 20180903
IS  - 1537-6524 (Electronic)
IS  - 1537-6516 (Linking)
VI  - 28
IP  - 4
DP  - 2018 May
TI  - Cellular effects of thirdhand tobacco smoke from smokers' homes.
PG  - 243-251
LID - 10.1080/15376516.2017.1411411 [doi]
AB  - After the cigarette is extinguished, many toxic compounds remain in the 
      environment and accumulate in the air or on surfaces. This exposure is termed 
      thirdhand smoke (THS) and its risks are poorly known. The aim of the study was to 
      evaluate the cellular effects of THS from smokers' homes. Papers were placed in 
      nine smoker's home and three nonsmoker's homes. An area equivalent to the paper 
      size was cleaned with a cotton wipe. A549, Hep-2 and 3T3 cells were exposed to 
      THS for 24 h and cellular functions were assessed by MTT, neutral red (NR) 
      reuptake and trypan blue exclusion assays. High levels of nicotine were found in 
      samples from smokers' homes. Cellular proliferation was similar in almost all 
      samples after THS exposure. Few changes in the cellular functions were observed, 
      mainly higher mitochondrial activity, in paper samples. We are able to detect 
      markers of THS collected from smokers' homes, but a clear evidence of cellular 
      toxicity cannot be demonstrated by the present assays. This is the first study to 
      evaluate the cellular effects of THS samples collected from smokers' homes.
FAU - Figueiro, Luciana Rizzieri
AU  - Figueiro LR
AUID- ORCID: 0000-0001-7224-8927
AD  - a PPG Pathology , Federal University of Health Sciences of Porto Alegre (UFCSPA) 
      , Porto Alegre , Brazil.
FAU - Linden, Rafael
AU  - Linden R
AD  - b Toxicology Laboratory, Institute of Health Sciences , Feevale University , Novo 
      Hamburgo , Brazil.
FAU - Ziulkoski, Ana Luiza
AU  - Ziulkoski AL
AD  - c Cytotoxicity Laboratory, Institute of Health Sciences , Feevale University , 
      Novo Hamburgo , Brazil.
FAU - Dantas, Denise Conceicao Mesquita
AU  - Dantas DCM
AD  - d Pharmacoscience Department , UFCSPA , Porto Alegre , Brazil.
LA  - eng
PT  - Journal Article
DEP - 20171207
PL  - England
TA  - Toxicol Mech Methods
JT  - Toxicology mechanisms and methods
JID - 101134521
RN  - 0 (Tobacco Smoke Pollution)
SB  - IM
MH  - 3T3 Cells
MH  - A549 Cells
MH  - Air Pollution, Indoor/*adverse effects/analysis
MH  - Animals
MH  - Brazil
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Chromatography, Paper
MH  - Environmental Monitoring/*methods
MH  - Gas Chromatography-Mass Spectrometry
MH  - *Housing/standards
MH  - Humans
MH  - Mice
MH  - Tobacco Smoke Pollution/*adverse effects/analysis
OTO - NOTNLM
OT  - Thirdhand tobacco smoke
OT  - cytotoxicity
OT  - environmental tobacco smoke
OT  - nicotine
OT  - smoke
OT  - tobacco
EDAT- 2017/12/01 06:00
MHDA- 2018/09/04 06:00
CRDT- 2017/11/30 06:00
PHST- 2017/12/01 06:00 [pubmed]
PHST- 2018/09/04 06:00 [medline]
PHST- 2017/11/30 06:00 [entrez]
AID - 10.1080/15376516.2017.1411411 [doi]
PST - ppublish
SO  - Toxicol Mech Methods. 2018 May;28(4):243-251. doi: 10.1080/15376516.2017.1411411. 
      Epub 2017 Dec 7.