PMID- 29186870
OWN - NLM
STAT- MEDLINE
DCOM- 20180703
LR  - 20221207
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 12
DP  - 2017 Nov 26
TI  - Dietary Supplementation with Lactobacillus casei Alleviates 
      Lipopolysaccharide-Induced Liver Injury in a Porcine Model.
LID - 10.3390/ijms18122535 [doi]
LID - 2535
AB  - This study aims to determine whether Lactobacillus casei (L. casei) could relieve 
      liver injury in piglets challenged with lipopolysaccharide (LPS). Piglets were 
      randomly allocated into one of the three groups: control, LPS, and L. casei. The 
      control and LPS groups were fed a corn- and soybean meal-based diet, whereas the 
      L. casei group was fed the basal diet supplemented with 6 x 10(6) cfu/g L. casei. 
      On Day 31 of the trial, piglets in the LPS and L. casei groups received 
      intraperitoneal administration of LPS (100 microg/kg body weight), while the control 
      group received the same volume of saline. Blood and liver samples were collected 
      for analysis. Results showed that L. casei supplementation decreased the 
      feed/gain ratio (p = 0.027) and diarrhea incidence (p < 0.001), and attenuated 
      LPS-induced liver histomorphological abnormalities. Compared with the control 
      group, LPS challenge dramatically increased glutamyl transpeptidase activity (p = 
      0.001) in plasma as well as the concentrations of Interleukin 6 (IL-6) (p = 
      0.048), Tumor necrosis factor-alpha (TNF-alpha) (p = 0.041), and Malondialdehyde 
      (MDA) (p = 0.001) in the liver, while decreasing the hepatic SOD activity. LPS 
      also increased (p < 0.05) the mRNA levels for IL-6, IL-8, TNF-alpha, Toll-like 
      receptors 4 (TLR4), Nuclear factor kappaB (NF-kappaB) and Heat shock protein 70 (HSP70) 
      in the liver. The adverse effects of LPS challenge were ameliorated by L. casei 
      supplementation. In conclusion, dietary L. casei alleviates LPS-induced liver 
      injury via reducing pro-inflammatory cytokines and increasing anti-oxidative 
      capacity.
FAU - Zhao, Di
AU  - Zhao D
AD  - Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei 
      Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic 
      University, Wuhan 430023, China. zhaodi@whpu.edu.cn.
FAU - Wu, Tao
AU  - Wu T
AD  - Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei 
      Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic 
      University, Wuhan 430023, China. wutao@whpu.edu.cn.
FAU - Yi, Dan
AU  - Yi D
AD  - Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei 
      Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic 
      University, Wuhan 430023, China. yidan810204@whpu.edu.cn.
FAU - Wang, Lei
AU  - Wang L
AD  - Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei 
      Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic 
      University, Wuhan 430023, China. wanglei@whpu.edu.cn.
FAU - Li, Peng
AU  - Li P
AD  - Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei 
      Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic 
      University, Wuhan 430023, China. Lipeng@whpu.edu.cn.
FAU - Zhang, Junmei
AU  - Zhang J
AD  - Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei 
      Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic 
      University, Wuhan 430023, China. zhangjm@whpu.edu.cn.
FAU - Hou, Yongqing
AU  - Hou Y
AD  - Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei 
      Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic 
      University, Wuhan 430023, China. houyq@whpu.edu.cn.
FAU - Wu, Guoyao
AU  - Wu G
AD  - Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei 
      Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic 
      University, Wuhan 430023, China. g-wu@exchange.tamu.edu.
AD  - Department of Animal Science, Texas A & M University, College Station, TX 77843, 
      USA. g-wu@exchange.tamu.edu.
LA  - eng
PT  - Journal Article
DEP - 20171126
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 2.3.2.2 (gamma-Glutamyltransferase)
SB  - IM
MH  - Animals
MH  - Dietary Supplements
MH  - Female
MH  - HSP70 Heat-Shock Proteins/metabolism
MH  - Interleukin-6/blood
MH  - *Lacticaseibacillus casei
MH  - Lipopolysaccharides/*toxicity
MH  - Liver/metabolism
MH  - Liver Diseases/etiology/*therapy
MH  - Malondialdehyde/metabolism
MH  - NF-kappa B/metabolism
MH  - Probiotics/administration & dosage/*therapeutic use
MH  - Swine
MH  - Tumor Necrosis Factor-alpha/blood
MH  - gamma-Glutamyltransferase/blood
PMC - PMC5751138
OTO - NOTNLM
OT  - Lactobacillus casei
OT  - lipopolysaccharide
OT  - liver
OT  - piglets
COIS- The authors declare no conflict of interest.
EDAT- 2017/12/01 06:00
MHDA- 2018/07/04 06:00
PMCR- 2017/12/01
CRDT- 2017/12/01 06:00
PHST- 2017/10/30 00:00 [received]
PHST- 2017/11/20 00:00 [revised]
PHST- 2017/11/21 00:00 [accepted]
PHST- 2017/12/01 06:00 [entrez]
PHST- 2017/12/01 06:00 [pubmed]
PHST- 2018/07/04 06:00 [medline]
PHST- 2017/12/01 00:00 [pmc-release]
AID - ijms18122535 [pii]
AID - ijms-18-02535 [pii]
AID - 10.3390/ijms18122535 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Nov 26;18(12):2535. doi: 10.3390/ijms18122535.