PMID- 29190603
OWN - NLM
STAT- MEDLINE
DCOM- 20180716
LR  - 20180716
IS  - 1735-8604 (Electronic)
IS  - 1735-8582 (Linking)
VI  - 11
IP  - 6
DP  - 2017 Nov
TI  - Upregulation of Cabin1 During Injury to Renal Tubular Epithelial Cells in Rats.
PG  - 430-437
AB  - INTRODUCTION: Calcineurin-binding protein 1 (Cabin1) interacts with calcineurin 
      and p53, but its function in renal tubular epithelial cell (RTEC) is unclear. We 
      established 5/6 nephrectomized rats and angiotensin II-induced injury to the 
      RTECs in vitro, to observe the expression of Cabin1 during RTEC injury. MATERIALS 
      AND METHODS: Sprague-Dawley rats were sacrificed at 4 and 8 weeks after 5/6 
      nephrectomy. Renal pathology and mitochondrial damage were detected by light and 
      electrical microscope. The distribution of E-cadherin and alpha-smad were detected by 
      indirect immunofluorescence staining. Cabin1 protein expression was detected by 
      Western blot. RESULTS: Obvious tubulointerstitial fibrosis was found in the 
      nephrectomized rats at 8 weeks after 5/6 nephrectomy, accompanied by the 
      increasing levels of creatinine, as well as the disruption of E-cadherin and 
      overexpression of alpha-smad in RTECs. Moreover, the mitochondria became swollen and 
      mitochondrial cristae were disrupted and poorly defined in the RTECs. Compared to 
      the sham-operated rats, Cabin1 protein expression was significantly increased at 
      8 weeks after 5/6 nephrectomy, while angiotensin II-induced Cabin1 protein 
      expression significantly increased 48 hours after stimulation in normal rat 
      kidney epithelial cells. CONCLUSIONS: Injury to the RTEC and Cabin1 protein 
      overexpression occurred in a time-dependent manner both in vitro and in vivo. 
      Cabin1 may become a potential molecular target in RTEC injury.
FAU - Wen, Yueqiang
AU  - Wen Y
AD  - Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical 
      University, Guangzhou, China. yueqiangwen@163.com.
FAU - Li, Huiyuan
AU  - Li H
FAU - Zhou, Peilan
AU  - Zhou P
FAU - Liu, Lingling
AU  - Liu L
FAU - Wang, Zebin
AU  - Wang Z
FAU - Liang, Jianbo
AU  - Liang J
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Kidney Dis
JT  - Iranian journal of kidney diseases
JID - 101316967
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (CDH1 protein, rat)
RN  - 0 (Cabin1 protein, rat)
RN  - 0 (Cadherins)
RN  - 0 (Smad Proteins)
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Angiotensin II/toxicity
MH  - Animals
MH  - Apoptosis Regulatory Proteins/*metabolism
MH  - Cadherins/metabolism
MH  - Cell Line
MH  - Disease Models, Animal
MH  - Epithelial Cells/drug effects/*metabolism/ultrastructure
MH  - Fibrosis
MH  - Kidney Diseases/*metabolism/pathology
MH  - Kidney Tubules/drug effects/*metabolism/ultrastructure
MH  - Mitochondria/drug effects/metabolism/ultrastructure
MH  - Mitochondrial Swelling
MH  - Nephrectomy
MH  - Rats, Sprague-Dawley
MH  - Smad Proteins/metabolism
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2017/12/01 06:00
MHDA- 2018/07/17 06:00
CRDT- 2017/12/01 06:00
PHST- 2017/01/13 00:00 [received]
PHST- 2017/04/13 00:00 [accepted]
PHST- 2017/12/01 06:00 [entrez]
PHST- 2017/12/01 06:00 [pubmed]
PHST- 2018/07/17 06:00 [medline]
AID - 3183/966 [pii]
PST - ppublish
SO  - Iran J Kidney Dis. 2017 Nov;11(6):430-437.