PMID- 2919120
OWN - NLM
STAT- MEDLINE
DCOM- 19890405
LR  - 20061115
IS  - 0031-3998 (Print)
IS  - 0031-3998 (Linking)
VI  - 25
IP  - 1
DP  - 1989 Jan
TI  - Human lactation. II: Endogenous fatty acid synthesis by the mammary gland.
PG  - 63-8
AB  - We studied the effects of a diet that was low in fat, high in carbohydrate (CHO) 
      on milk lipid composition and de novo endogenous fatty acid synthesis by the 
      mammary gland in five lactating women. The women consumed either a low fat (LF) 
      (5% fat, 80% CHO) diet or a high fat (HF) (40% fat, 45% CHO) diet. Fat synthesis 
      was determined after an oral dose of 500 mg/kg D2O by measuring the incorporation 
      of deuterium into C10:0 to C18:0 saturated fatty acids of milk fat and plasma 
      triglycerides by gas chromatography-mass spectrometry. Synthesis of plasma C16:0 
      and C18:0 triglycerides was barely detectable while women consumed the HF diet, 
      but was increased 6-fold during the LF diet. Medium chain fatty acids secreted by 
      the mammary gland increased from 12.8% (HF diet) to 16.3% (LF diet) in milk fat 
      from four of five subjects (p = 0.027). Medium chain fatty acid secretion, 
      however, increased from 13.9% (HF diet) to 29.9% (LF diet) in one subject. The 
      primary fatty acids synthesized during lactation were C10:0, C12:0, and C14:0 in 
      the majority of women studied. The LF diet significantly increased the apparent 
      synthesis of C14:0 (p = 0.05), whereas no changes were observed in C12:0, C16:0, 
      or C18:0. One subject had highly enriched C16:0 and C18:0 fatty acids in her milk 
      on the LF diet, which could have been the result of mammary synthesis or of 
      transport and secretion of hepatically synthesized lipids.
FAU - Hachey, D L
AU  - Hachey DL
AD  - Department of Pediatrics, Baylor College of Medicine, Houston, Texas.
FAU - Silber, G H
AU  - Silber GH
FAU - Wong, W W
AU  - Wong WW
FAU - Garza, C
AU  - Garza C
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Fats)
RN  - 0 (Fatty Acids)
RN  - 0 (Lipids)
SB  - IM
MH  - Body Water/analysis
MH  - Body Weight
MH  - Breast/*metabolism
MH  - Dietary Carbohydrates/*administration & dosage
MH  - Dietary Fats/*administration & dosage
MH  - Fatty Acids/*biosynthesis
MH  - Female
MH  - Humans
MH  - Lactation/*metabolism
MH  - Lipids/blood
MH  - Milk, Human/analysis/metabolism
MH  - Pregnancy
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 10.1203/00006450-198901000-00015 [doi]
PST - ppublish
SO  - Pediatr Res. 1989 Jan;25(1):63-8. doi: 10.1203/00006450-198901000-00015.