PMID- 29191214
OWN - NLM
STAT- MEDLINE
DCOM- 20180731
LR  - 20181113
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 19
IP  - 1
DP  - 2017 Dec 1
TI  - The role of aberrant expression of T cell miRNAs affected by TNF-alpha in the 
      immunopathogenesis of rheumatoid arthritis.
PG  - 261
LID - 10.1186/s13075-017-1465-z [doi]
LID - 261
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) can cause diverse T cell 
      dysfunctions in patients with rheumatoid arthritis (RA). It is involved in the 
      regulation of microRNAs (miRNAs) expression in different cell types. We 
      hypothesized that the expression of T cell miRNAs would be affected by TNF-alpha, and 
      these miRNAs could participate in the immunopathogenesis of RA. METHODS: 
      Expression profiles of 270 human miRNAs in Jurkat cells, cultured in the presence 
      or absence of TNF-alpha for 7 days were analyzed by real-time polymerase chain 
      reaction. Potentially aberrantly expressed miRNAs were validated using T cell 
      samples from 35 patients with RA and 15 controls. Transfection studies were 
      conducted to search for gene expression and biological functions regulated by 
      specific miRNAs. RESULTS: Initial analysis revealed 12 miRNAs were significantly 
      lower, whereas the expression level of miR-146a was significantly higher in 
      Jurkat cells after being cultured with TNF-alpha for 7 days. Decreased expression of 
      miR-139-3p, miR-204, miR-760, miR-524-5p, miR-136, miR-548d-3p, miR-214, miR-383, 
      and miR-887 were noted in RA T cells. Expression levels of miR-139-3p, miR-204, 
      miR-214, and miR-760 were correlated with the use of biologic agents. The 
      transfection of miR-214 mimic suppressed TNF-alpha-mediated apoptosis of Jurkat 
      cells. Increased phosphorylation of extracellular regulating kinase (ERK) and 
      c-Jun N-terminal kinase (JNK) was noted in RA T cells and Jurkat cells after 
      TNF-alpha exposure. Transfection of Jurkat cells with miR-214 mimic suppressed both 
      the basal and TNF-alpha-mediated ERK and JNK phosphoryation. CONCLUSIONS: Among T 
      cell miRNAs affected by TNF-alpha, the expression levels of nine miRNAs were 
      decreased in T cells from patients with RA. The expression levels of miR-139-3p, 
      miR-204, miR-214, and miR-760 increased in RA patients receiving biologic agents. 
      The transfection of miR-214 reversed the TNF-alpha-mediated cells apoptosis and 
      inhibited the phosphorylation of ERK and JNK in Jurkat cells.
FAU - Lai, Ning-Sheng
AU  - Lai NS
AD  - Division of Allergy, Immunology and Rheumatology, Dalin Tzu Chi Hospital, 
      Buddhist Tzu Chi Medical Foundation, No. 2, Minsheng Road, Dalin, Chiayi, 62247, 
      Taiwan.
AD  - School of Medicine, Tzu Chi University, Hualien City, Taiwan.
FAU - Yu, Hui-Chun
AU  - Yu HC
AD  - Division of Allergy, Immunology and Rheumatology, Dalin Tzu Chi Hospital, 
      Buddhist Tzu Chi Medical Foundation, No. 2, Minsheng Road, Dalin, Chiayi, 62247, 
      Taiwan.
FAU - Tung, Chien-Hsueh
AU  - Tung CH
AD  - Division of Allergy, Immunology and Rheumatology, Dalin Tzu Chi Hospital, 
      Buddhist Tzu Chi Medical Foundation, No. 2, Minsheng Road, Dalin, Chiayi, 62247, 
      Taiwan.
FAU - Huang, Kuang-Yung
AU  - Huang KY
AD  - Division of Allergy, Immunology and Rheumatology, Dalin Tzu Chi Hospital, 
      Buddhist Tzu Chi Medical Foundation, No. 2, Minsheng Road, Dalin, Chiayi, 62247, 
      Taiwan.
AD  - School of Medicine, Tzu Chi University, Hualien City, Taiwan.
FAU - Huang, Hsien-Bin
AU  - Huang HB
AD  - Department of Life Science and Institute of Molecular Biology, National Chung 
      Cheng University, Minxiong, Chiayi, Taiwan.
FAU - Lu, Ming-Chi
AU  - Lu MC
AUID- ORCID: 0000-0001-9051-0351
AD  - Division of Allergy, Immunology and Rheumatology, Dalin Tzu Chi Hospital, 
      Buddhist Tzu Chi Medical Foundation, No. 2, Minsheng Road, Dalin, Chiayi, 62247, 
      Taiwan. e360187@yahoo.com.tw.
AD  - School of Medicine, Tzu Chi University, Hualien City, Taiwan. 
      e360187@yahoo.com.tw.
LA  - eng
GR  - MOST 104-2314-B-303-008-MY2/Ministry of Science and Technology, Taiwan/
PT  - Journal Article
DEP - 20171201
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 0 (MicroRNAs)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Apoptosis/immunology
MH  - Arthritis, Rheumatoid/*immunology
MH  - Gene Expression Regulation/*immunology
MH  - Humans
MH  - Jurkat Cells
MH  - MicroRNAs/*immunology
MH  - T-Lymphocytes/*immunology
MH  - Tumor Necrosis Factor-alpha/*immunology
PMC - PMC5709919
OTO - NOTNLM
OT  - Apoptosis
OT  - ERK
OT  - JNK
OT  - Rheumatoid arthritis
OT  - T cells
OT  - TNF-alpha
OT  - microRNAs
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: This study was conducted in 
      accordance with the Declaration of Helsinki and was approved by the institutional 
      review board of Buddhist Dalin Tzu Chi Hospital, Taiwan (No. B10503007). Signed 
      informed consent was obtained from all patients. CONSENT FOR PUBLICATION: Not 
      applicable. COMPETING INTERESTS: The authors declare that they have no competing 
      interests.
EDAT- 2017/12/02 06:00
MHDA- 2018/08/01 06:00
PMCR- 2017/12/01
CRDT- 2017/12/02 06:00
PHST- 2017/06/28 00:00 [received]
PHST- 2017/11/06 00:00 [accepted]
PHST- 2017/12/02 06:00 [entrez]
PHST- 2017/12/02 06:00 [pubmed]
PHST- 2018/08/01 06:00 [medline]
PHST- 2017/12/01 00:00 [pmc-release]
AID - 10.1186/s13075-017-1465-z [pii]
AID - 1465 [pii]
AID - 10.1186/s13075-017-1465-z [doi]
PST - epublish
SO  - Arthritis Res Ther. 2017 Dec 1;19(1):261. doi: 10.1186/s13075-017-1465-z.