PMID- 29195146
OWN - NLM
STAT- MEDLINE
DCOM- 20180221
LR  - 20180221
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1072
DP  - 2018 Jan 1
TI  - Fast and quantitative determination of saxitoxin and neosaxitoxin in urine by 
      ultra performance liquid chromatography-triple quadrupole mass spectrometry based 
      on the cleanup of solid phase extraction with hydrophilic interaction mechanism.
PG  - 267-272
LID - S1570-0232(17)30998-4 [pii]
LID - 10.1016/j.jchromb.2017.11.032 [doi]
AB  - Saxitoxin (STX) and neosaxitoxin (NEO) are water-soluble toxins and their cleanup 
      in bio-matrix is a hot topic but difficult problem. A fast and quantitative 
      determination method for STX and NEO in urine was developed using ultra 
      performance liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) 
      based on the cleanup of solid phase extraction (SPE) with hydrophilic interaction 
      (HILIC) mechanism. Acetonitrile/methanol/water mixture was used to extract the 
      toxins in urine. Polyamide (PA) was used as HILIC SPE material to clean the 
      toxins in sample matrix. The limits of detection were 0.2ngmL(-1) for STX and 
      1ngmL(-1) for NEO in urine. The linear ranges were 0.5ngmL(-1)-99.2ngmL(-1) with 
      the correlation coefficient of r=0.9992 for STX and 2.1ngmL(-1)-207ngmL(-1) with 
      r=0.997 for NEO in urine matrix. The recoveries at three spiking levels were 
      81.5%-117% with the relative standard deviations (RSDs) of 5.4%-8.5% for STX and 
      89.0%-118% with the RSDs of 6.7%-9.1% for NEO. STX was found in all the 6 
      patients' urines while NEO was only found in one sample from an intoxication 
      case.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Xu, Xiao-Min
AU  - Xu XM
AD  - Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, 
      China. Electronic address: chemxuxm@163.com.
FAU - Huang, Bai-Fen
AU  - Huang BF
AD  - Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, 
      China.
FAU - Xu, Jiao-Jiao
AU  - Xu JJ
AD  - Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, 
      China.
FAU - Cai, Zeng-Xuan
AU  - Cai ZX
AD  - Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, 
      China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, 
      China.
FAU - Chen, Qing
AU  - Chen Q
AD  - Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, 
      China.
FAU - Han, Jian-Long
AU  - Han JL
AD  - Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, 
      China. Electronic address: jlhan@cdc.zj.cn.
LA  - eng
PT  - Journal Article
DEP - 20171126
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 35523-89-8 (Saxitoxin)
RN  - 6YRL8BWD9H (neosaxitoxin)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Limit of Detection
MH  - Linear Models
MH  - Reproducibility of Results
MH  - Saxitoxin/*analogs & derivatives/chemistry/isolation & purification/*urine
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Hydrophilic interaction
OT  - Liquid chromatography-triple quadrupole mass spectrometry
OT  - Neosaxitoxin
OT  - Saxitoxin
OT  - Solid phase extraction
OT  - Urine
EDAT- 2017/12/02 06:00
MHDA- 2018/02/22 06:00
CRDT- 2017/12/02 06:00
PHST- 2017/06/01 00:00 [received]
PHST- 2017/11/22 00:00 [revised]
PHST- 2017/11/24 00:00 [accepted]
PHST- 2017/12/02 06:00 [pubmed]
PHST- 2018/02/22 06:00 [medline]
PHST- 2017/12/02 06:00 [entrez]
AID - S1570-0232(17)30998-4 [pii]
AID - 10.1016/j.jchromb.2017.11.032 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 1;1072:267-272. doi: 
      10.1016/j.jchromb.2017.11.032. Epub 2017 Nov 26.