PMID- 29197248
OWN - NLM
STAT- MEDLINE
DCOM- 20180416
LR  - 20201209
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 194
DP  - 2018 Mar
TI  - The role of IL-8/CXCR2 signaling in microcystin-LR triggered endothelial cell 
      activation and increased vascular permeability.
PG  - 43-48
LID - S0045-6535(17)31897-0 [pii]
LID - 10.1016/j.chemosphere.2017.11.120 [doi]
AB  - Microcystins are a family of cyclic heptapeptide toxins naturally produced by 
      freshwater cyanobacteria. Microcystin-LR (MCLR) is believed to be the most toxic 
      and common one with various pathological effects on human and mammals. However, 
      the effects of MCLR on endothelial cells and vascular homeostasis have been 
      largely unknown. We explored the mRNA and protein expression changes of several 
      pro-inflammatory mediators in human umbilical vein endothelial cells (HUVECs) and 
      C57BC/6 mice exposed to MCLR. Tumor necrosis factor-alpha (TNF-alpha), interleukin-1 
      beta (IL-1beta), interleukin-6 (IL-6), especially interleukin-8 (IL-8) were 
      remarkably upregulated both in endothelial cells and in serum. Increased 
      endothelial permeability in vitro and chronic microvascular permeability in 
      animals were also observed. Silencing the IL-8 gene with siRNA or blocking its 
      cognate receptor, CXC-chemokine receptor type 2 (CXCR2), by a specific inhibitor 
      efficiently prevented the MCLR induced leakage. These observations indicate a 
      novel insight of inflammation triggered property of MCLR via IL-8/CXCR2 
      signaling, suggesting CXCR2 as a target molecule in protective strategy against 
      the wide range pollution of microcystin.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Chen, Limei
AU  - Chen L
AD  - Department of Marine Ecology, College of Marine Life Sciences, Ocean University 
      of China, Qingdao, 266003, China; School of Bioscience and Technology, Weifang 
      Medical University, Weifang, 261053, China. Electronic address: 
      chenlimei2005@163.conm.
FAU - Liu, Xiaoying
AU  - Liu X
AD  - School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, 
      China. Electronic address: xiaoying6690@163.com.
FAU - Pan, Zhifang
AU  - Pan Z
AD  - School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, 
      China. Electronic address: pzhifang@163.com.
FAU - Liu, Shunmei
AU  - Liu S
AD  - School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, 
      China. Electronic address: bio-shunmei@163.com.
FAU - Han, Huirong
AU  - Han H
AD  - School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, 
      China. Electronic address: hanhuirong@wfmc.edu.cn.
FAU - Zhao, Chunling
AU  - Zhao C
AD  - School of Bioscience and Technology, Weifang Medical University, Weifang, 261053, 
      China. Electronic address: zhaochunlingbj@163.com.
FAU - Tang, Xuexi
AU  - Tang X
AD  - Department of Marine Ecology, College of Marine Life Sciences, Ocean University 
      of China, Qingdao, 266003, China. Electronic address: tangxx@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20171125
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - 0 (Receptors, Interleukin-8B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EQ8332842Y (cyanoginosin LR)
SB  - IM
MH  - Animals
MH  - Capillary Permeability/*drug effects
MH  - Cyanobacteria/metabolism/pathogenicity
MH  - Human Umbilical Vein Endothelial Cells/*metabolism
MH  - Humans
MH  - Inflammation/complications/etiology
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/genetics/*metabolism
MH  - Marine Toxins
MH  - Mice
MH  - Microcystins/*pharmacology
MH  - Receptors, Interleukin-8B/*metabolism
MH  - Signal Transduction/drug effects
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - IL-8/CXCR2 signal
OT  - Microcystin-LR
OT  - Pro-inflammatory mediators
OT  - Vascular permeability
EDAT- 2017/12/03 06:00
MHDA- 2018/04/17 06:00
CRDT- 2017/12/03 06:00
PHST- 2017/08/19 00:00 [received]
PHST- 2017/11/06 00:00 [revised]
PHST- 2017/11/21 00:00 [accepted]
PHST- 2017/12/03 06:00 [pubmed]
PHST- 2018/04/17 06:00 [medline]
PHST- 2017/12/03 06:00 [entrez]
AID - S0045-6535(17)31897-0 [pii]
AID - 10.1016/j.chemosphere.2017.11.120 [doi]
PST - ppublish
SO  - Chemosphere. 2018 Mar;194:43-48. doi: 10.1016/j.chemosphere.2017.11.120. Epub 
      2017 Nov 25.