PMID- 29197873
OWN - NLM
STAT- MEDLINE
DCOM- 20180618
LR  - 20180618
IS  - 1423-0135 (Electronic)
IS  - 1018-1172 (Linking)
VI  - 55
IP  - 1
DP  - 2018
TI  - Comparative Anti-Platelet Profiling Reveals a Potent Anti-Aggregatory Effect of 
      CD34+ Progenitor Cell-Derived Late-Outgrowth Endothelial Cells in vitro.
PG  - 13-25
LID - 10.1159/000481779 [doi]
AB  - BACKGROUND/AIMS: Platelets affect endothelial progenitor cell (EPC) 
      functionality, inducing their differentiation into mature endothelial cells. 
      However, it remains to be established whether EPCs can influence platelet 
      functionality. METHODS: Mononuclear proangiogenic cells (MPCs) and early 
      outgrowth cells (EOCs) were prepared from peripheral blood mononuclear cells, 
      whereas late-outgrowth endothelial cells (OECs) were prepared from cord blood 
      CD34+ cells. The effect of the above cells and their supernatants on washed 
      platelet aggregation was studied. The effect of OECs and their supernatant on the 
      adenosine diphosphate (ADP)-induced magnitude of platelet integrin receptor 
      alphaIIbbeta3 activation and on P-selectin membrane expression was investigated. The 
      levels of nitric oxide (NO) and prostacyclin (PGI2) that were secreted from EOCs, 
      OECs, and human umbilical vein endothelial cells (HUVECs) were also assessed. 
      RESULTS: Among all progenitors, OECs and their supernatant exhibited the most 
      potent inhibitory activity towards platelet aggregation. Furthermore, OECs and 
      their supernatant, but not CD34+ cells, reduced alphaIIbbeta3 activation and P-selectin 
      membrane expression. Finally, OECs secreted higher NO and PGI2 levels than EOCs 
      did. CONCLUSION: The anti-platelet effect of EPCs depends highly on the degree of 
      their endothelial phenotype, with OECs expressing the highest potency. Therefore, 
      the induction of OEC generation and the enhancement of their functionality in 
      vivo could be a new approach for the treatment of patients at a high thrombotic 
      risk.
CI  - (c) 2017 S. Karger AG, Basel.
FAU - Chantzichristos, Vasileios G
AU  - Chantzichristos VG
AD  - Atherothrombosis Research Centre/Laboratory of Biochemistry, Department of 
      Chemistry, School of Medicine, University of Ioannina, Ioannina, Greece.
FAU - Gkrozou, Fani
AU  - Gkrozou F
FAU - Stellos, Konstantinos
AU  - Stellos K
FAU - Paschopoulos, Minas E
AU  - Paschopoulos ME
FAU - Tselepis, Alexandros D
AU  - Tselepis AD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171202
PL  - Switzerland
TA  - J Vasc Res
JT  - Journal of vascular research
JID - 9206092
RN  - 0 (Antigens, CD34)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (P-Selectin)
RN  - 0 (Platelet Glycoprotein GPIIb-IIIa Complex)
RN  - 0 (SELP protein, human)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - DCR9Z582X0 (Epoprostenol)
SB  - IM
MH  - Adult
MH  - Antigens, CD34/*metabolism
MH  - Blood Platelets/*metabolism
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/metabolism
MH  - Endothelial Progenitor Cells/*metabolism
MH  - Epoprostenol/metabolism
MH  - Female
MH  - Fetal Blood/cytology
MH  - Human Umbilical Vein Endothelial Cells/metabolism
MH  - Humans
MH  - Nitric Oxide/metabolism
MH  - P-Selectin/metabolism
MH  - Phenotype
MH  - *Platelet Aggregation
MH  - Platelet Glycoprotein GPIIb-IIIa Complex/metabolism
MH  - Pregnancy
MH  - Signal Transduction
MH  - Time Factors
OTO - NOTNLM
OT  - CD34+ cells
OT  - Endothelial cells
OT  - Endothelial progenitor cells
OT  - Human umbilical vein endothelial cells
OT  - Late-outgrowth endothelial cells
OT  - Nitric oxide
OT  - Platelet activation
OT  - Platelets
OT  - Prostacyclin
EDAT- 2017/12/05 06:00
MHDA- 2018/06/19 06:00
CRDT- 2017/12/04 06:00
PHST- 2017/01/30 00:00 [received]
PHST- 2017/09/23 00:00 [accepted]
PHST- 2017/12/05 06:00 [pubmed]
PHST- 2018/06/19 06:00 [medline]
PHST- 2017/12/04 06:00 [entrez]
AID - 000481779 [pii]
AID - 10.1159/000481779 [doi]
PST - ppublish
SO  - J Vasc Res. 2018;55(1):13-25. doi: 10.1159/000481779. Epub 2017 Dec 2.