PMID- 29198124
OWN - NLM
STAT- MEDLINE
DCOM- 20180315
LR  - 20181202
IS  - 0006-9248 (Print)
IS  - 0006-9248 (Linking)
VI  - 118
IP  - 10
DP  - 2017
TI  - Investigation of the effectiveness of ghrelin treatment in lung tissue of rats 
      with sepsis.
PG  - 585-590
LID - 10.4149/BLL_2017_112 [doi]
AB  - OBJECTIVE: We aimed to investigate the effects of exogenous ghrelin on cytokine 
      and ghrelin levels, oxidant parameters, and apoptotic genes in lung tissue during 
      sepsis. BACKGROUND: There was evidence that changes of apoptosis are linked with 
      morbidity and mortality in sepsis. There were scarce studies on the effect of 
      ghrelin on apoptotic genes and endogenous ghrelin levels during sepsis. METHODS: 
      Male Wistar albino rats 200-250 g were separated into four groups; Control, LPS 
      (5 mg/kg), Ghrelin (10 nmol/kg i.v.), and LPS+Ghrelin. Tumor necrosis 
      factor-alpha (TNF-alpha), interleukin-10 (IL-10), and ghrelin levels were determined 
      from lung tissue using enzyme-linked immunosorbent assay (ELISA). TNF-alpha, IL-10, 
      Bcl-2, and Bax gene expressions were calculated using quantitative real-time 
      polymerase chain reaction (RT-PCR), tissue superoxide dismutase enzyme (SOD) 
      activities and malondialdehyde (MDA) were determined spectrophotometerically. 
      RESULTS: TNF-alpha levels decreased in the LPS+Ghrelin group compared with the LPS (p 
      < 0.001). IL-10 and MDA levels were found highly significantly increased in the 
      LPS and LPS+Ghrelin groups (p < 0.05). Tissue SOD activities were higher in the 
      Ghrelin and LPS+Ghrelin group compared with the LPS (p < 0.05). TNF-alpha, and Bax 
      expression levels were increased in the LPS compared with the other groups. IL-10 
      expression levels were increased in the experimental groups compared with the 
      controls. Bcl-2 expression levels were increased in the Ghrelin and LPS+Ghrelin 
      compared with other groups. CONCLUSION: Ghrelin treatment attenuated LPS-induced 
      lung injury. Treatment with ghrelin had no impact on serum and tissue ghrelin 
      levels, but it decreased the level of proinflammatory cytokines. We found that 
      ghrelin treatment had an antioxidant effect on SOD levels. Also, ghrelin 
      decreased the activity of proapoptotic Bax and increased antiapoptotic Bcl-2. Our 
      findings suggest that administration of ghrelin may attenuate damage in lung 
      tissue during sepsis (Fig. 4, Ref. 33).
FAU - Yorulmaz, H
AU  - Yorulmaz H
FAU - Ozkok, E
AU  - Ozkok E
FAU - Ates, G
AU  - Ates G
FAU - Tamer, S
AU  - Tamer S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Bratisl Lek Listy
JT  - Bratislavske lekarske listy
JID - 0065324
RN  - 0 (Bax protein, rat)
RN  - 0 (Bcl2 protein, rat)
RN  - 0 (Cytokines)
RN  - 0 (Ghrelin)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 130068-27-8 (Interleukin-10)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects/genetics
MH  - Cytokines/drug effects/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Ghrelin/drug effects/metabolism/*pharmacology
MH  - Interleukin-10/genetics/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lung/*drug effects/metabolism
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/drug effects/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Sepsis/*metabolism
MH  - Superoxide Dismutase/drug effects/metabolism
MH  - Tumor Necrosis Factor-alpha/*drug effects/genetics/metabolism
MH  - bcl-2-Associated X Protein/drug effects/genetics
OTO - NOTNLM
OT  - apoptosis
OT  - cytokine
OT  - ghrelin lipopolysaccharide.
EDAT- 2017/12/05 06:00
MHDA- 2018/03/16 06:00
CRDT- 2017/12/05 06:00
PHST- 2017/12/05 06:00 [entrez]
PHST- 2017/12/05 06:00 [pubmed]
PHST- 2018/03/16 06:00 [medline]
AID - 10.4149/BLL_2017_112 [doi]
PST - ppublish
SO  - Bratisl Lek Listy. 2017;118(10):585-590. doi: 10.4149/BLL_2017_112.