PMID- 29202332
OWN - NLM
STAT- MEDLINE
DCOM- 20180614
LR  - 20180705
IS  - 1873-3344 (Electronic)
IS  - 0162-0134 (Linking)
VI  - 179
DP  - 2018 Feb
TI  - Chloro(triphenylphosphine)gold(I) a forefront reagent in gold chemistry as 
      apoptotic agent for cancer cells.
PG  - 107-120
LID - S0162-0134(17)30461-0 [pii]
LID - 10.1016/j.jinorgbio.2017.11.004 [doi]
AB  - The antiproliferative activity of the gold complex [Au(tpp)Cl] (1) 
      (tpp=triphenyphosphine) against human breast adenocarcinoma cells (MCF-7) and 
      normal human fetal lung fibroblast cells (MRC-5) was investigated. The compound 
      exhibits stronger activity against MCF-7 cells than cisplatin. The apoptotic 
      pathway, especially though the mitochondrion damage was concluded by cell cycle 
      arrest, flow cytometry using Annexin V-Fluorescein IsoThioCyanate (FITC) and 
      Propidium Iodide (PI) as indicators, assays and permeabilization of the 
      mitochondrial membrane tests. The molecular mechanism of action of 1 was further 
      studied by: (i) its catalytic activity on the oxidation of linoleic acid (an acid 
      that partakes in membrane fluidity) to hyperoxolinoleic acid by oxygen and (ii) 
      its binding affinity towards the calf thymus (CT) DNA. Since the deactivation of 
      cisplatin by glutathione (GSH), is related with the development of cell 
      resistance, the reaction of 1 with GSH was investigated by UV absorption 
      spectroscopy. The absence of micronucleus in cells confirms that the complex has 
      no in vitro toxicity. The in vivo genotoxicity caused by 1 was evaluated by 
      Allium cepa test.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Chrysouli, M P
AU  - Chrysouli MP
AD  - Inorganic and Analytical Chemistry, Department of Chemistry, University of 
      Ioannina, 45110 Ioannina, Greece.
FAU - Banti, C N
AU  - Banti CN
AD  - Inorganic and Analytical Chemistry, Department of Chemistry, University of 
      Ioannina, 45110 Ioannina, Greece. Electronic address: cbanti@cc.uoi.gr.
FAU - Kourkoumelis, N
AU  - Kourkoumelis N
AD  - Medical Physics Laboratory, Medical School, University of Ioannina, Greece.
FAU - Panayiotou, N
AU  - Panayiotou N
AD  - Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus.
FAU - Markopoulos, G S
AU  - Markopoulos GS
AD  - Laboratory of Biology, School of Medicine, Faculty of Health Sciences, University 
      of Ioannina, Greece.
FAU - Tasiopoulos, A J
AU  - Tasiopoulos AJ
AD  - Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus.
FAU - Hadjikakou, S K
AU  - Hadjikakou SK
AD  - Inorganic and Analytical Chemistry, Department of Chemistry, University of 
      Ioannina, 45110 Ioannina, Greece. Electronic address: shadjika@uoi.gr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171108
PL  - United States
TA  - J Inorg Biochem
JT  - Journal of inorganic biochemistry
JID - 7905788
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Coordination Complexes)
RN  - 7440-57-5 (Gold)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - 9KJL21T0QJ (Linoleic Acid)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/chemistry/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Cattle
MH  - Coordination Complexes/chemistry/*pharmacology
MH  - DNA/chemistry
MH  - DNA Damage/drug effects
MH  - G2 Phase Cell Cycle Checkpoints/drug effects
MH  - Glutathione/chemistry
MH  - Gold/*chemistry
MH  - Humans
MH  - Linoleic Acid/chemistry
MH  - Lipid Peroxidation/drug effects
MH  - MCF-7 Cells
MH  - Micronucleus Tests
MH  - Mitochondrial Membranes/drug effects
MH  - Mitotic Index
MH  - Molecular Docking Simulation
MH  - Onions/genetics
MH  - Permeability
OTO - NOTNLM
OT  - Apoptosis
OT  - Breast cancer cells
OT  - Gold(I) complexes
OT  - Medicinal chemistry
OT  - Metallotherapeutic
OT  - Pnictogen
EDAT- 2017/12/05 06:00
MHDA- 2018/06/15 06:00
CRDT- 2017/12/05 06:00
PHST- 2017/06/28 00:00 [received]
PHST- 2017/10/30 00:00 [revised]
PHST- 2017/11/04 00:00 [accepted]
PHST- 2017/12/05 06:00 [pubmed]
PHST- 2018/06/15 06:00 [medline]
PHST- 2017/12/05 06:00 [entrez]
AID - S0162-0134(17)30461-0 [pii]
AID - 10.1016/j.jinorgbio.2017.11.004 [doi]
PST - ppublish
SO  - J Inorg Biochem. 2018 Feb;179:107-120. doi: 10.1016/j.jinorgbio.2017.11.004. Epub 
      2017 Nov 8.