PMID- 29203334
OWN - NLM
STAT- MEDLINE
DCOM- 20180911
LR  - 20180911
IS  - 1872-7549 (Electronic)
IS  - 0166-4328 (Linking)
VI  - 346
DP  - 2018 Jul 2
TI  - Sex differences in olfactory-induced neural activation of the amygdala.
PG  - 96-104
LID - S0166-4328(17)31378-5 [pii]
LID - 10.1016/j.bbr.2017.11.034 [doi]
AB  - Olfactory signals, including the scent of urine, are thought to be processed by 
      specific brain regions, such as the medial amygdala (Me), and regulate sexual 
      behavior in a sex-dependent manner. We aimed to reveal the sex-specific neural 
      circuit from the accessory olfactory bulb (AOB) to Me by using a transgenic 
      mouse. We quantified the long-lasting green fluorescent protein (GFP) expression 
      profile, which was controlled by the c-fos promotor in a sex-dependent manner by 
      the scent of urine. Female urine predominantly activated neurons of the 
      posterodorsal medial amygdala (MePD) in male mice and the posteroventral medial 
      amygdala (MePV) in female mice. Male urine, in contrast, generated the opposite 
      pattern of activation in the Me. Secondary, the selective artificial activation 
      of these circuits was used to examine their specific behavioral function, by 
      using a dual Cre-loxP viral infection. AAV-hSyn-FLEX-hM3Dq-EGFP-the designer 
      receptor exclusively activated by a designer drug-was infused into the AOB after 
      infection with trans-synaptic AAV(DJ)-CMV-mCherry-2A-Cre-TTC into either the MePD 
      or the MePV. Double virus-transfected mice were injected with hM(3)Dq activator 
      and their sexual behavior was monitored. However, selective activation of 
      sex-dependent circuits, i.e., the AOB-MePD or AOB-MePV, did not significantly 
      alter mounting or attack behavior in male mice. There were clear sex differences 
      in the pheromone conveying circuits in the AOB-Me of mice. The sex-dependent 
      functional activation of the Me, however, no effect on behavior. This suggests 
      that a diverse number of nuclei and brain areas are likely to function in concert 
      to successfully facilitate sexual and aggressive behaviors.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Kikusui, Takefumi
AU  - Kikusui T
AD  - Companion Animal Research, School of Veterinary Medicine, Azabu University, 
      Sagamihara 252-5201, Japan.
FAU - Kajita, Mayu
AU  - Kajita M
AD  - Companion Animal Research, School of Veterinary Medicine, Azabu University, 
      Sagamihara 252-5201, Japan.
FAU - Otsuka, Natsumi
AU  - Otsuka N
AD  - Companion Animal Research, School of Veterinary Medicine, Azabu University, 
      Sagamihara 252-5201, Japan.
FAU - Hattori, Tatsuya
AU  - Hattori T
AD  - Companion Animal Research, School of Veterinary Medicine, Azabu University, 
      Sagamihara 252-5201, Japan.
FAU - Kumazawa, Kanako
AU  - Kumazawa K
AD  - Companion Animal Research, School of Veterinary Medicine, Azabu University, 
      Sagamihara 252-5201, Japan.
FAU - Watarai, Akiyuki
AU  - Watarai A
AD  - Companion Animal Research, School of Veterinary Medicine, Azabu University, 
      Sagamihara 252-5201, Japan.
FAU - Nagasawa, Miho
AU  - Nagasawa M
AD  - Companion Animal Research, School of Veterinary Medicine, Azabu University, 
      Sagamihara 252-5201, Japan; Division of Brain and Neurophysiology, Department of 
      Physiology, Jichi Medical University, Yakushiji 3311-1, Shimotsuke, Tochigi 
      329-0498, Japan.
FAU - Inutsuka, Ayumu
AU  - Inutsuka A
AD  - Department of Neuroscience II, Research Institute of Environmental Medicine, 
      Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan; Division of 
      Brain and Neurophysiology, Department of Physiology, Jichi Medical University, 
      Yakushiji 3311-1, Shimotsuke, Tochigi 329-0498, Japan.
FAU - Yamanaka, Akihiro
AU  - Yamanaka A
AD  - Department of Neuroscience II, Research Institute of Environmental Medicine, 
      Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
FAU - Matsuo, Naoki
AU  - Matsuo N
AD  - Department of Molecular and Behavioral Neuroscience, Graduate School of Medicine, 
      Osaka University, Suita 565-0871, Japan.
FAU - Covington, Herbert E 3rd
AU  - Covington HE 3rd
AD  - Laboratory of Psychopharmacology, Department of Psychology, Tufts University,530 
      Boston Avenue, Medford, MA 02155, USA.
FAU - Mogi, Kazutaka
AU  - Mogi K
AD  - Companion Animal Research, School of Veterinary Medicine, Azabu University, 
      Sagamihara 252-5201, Japan. Electronic address: mogik@azabu-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171202
PL  - Netherlands
TA  - Behav Brain Res
JT  - Behavioural brain research
JID - 8004872
RN  - 0 (Early Growth Response Protein 1)
RN  - 0 (Egr1 protein, mouse)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Amygdala/cytology/*physiology
MH  - Animals
MH  - Early Growth Response Protein 1/metabolism
MH  - Female
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Neurons/cytology/drug effects/*physiology
MH  - Olfactory Bulb/cytology/drug effects/*physiology
MH  - Olfactory Pathways/cytology/physiology
MH  - Olfactory Perception/*physiology
MH  - *Sex Characteristics
MH  - Social Behavior
MH  - *Social Perception
MH  - Urine
OTO - NOTNLM
OT  - Accessory olfactory bulb
OT  - Designer receptor exclusively activated by designer drug
OT  - Medial amygdala
OT  - Sex difference
OT  - Social behavior
OT  - Vomeronasal system
EDAT- 2017/12/06 06:00
MHDA- 2018/09/12 06:00
CRDT- 2017/12/06 06:00
PHST- 2017/08/17 00:00 [received]
PHST- 2017/11/21 00:00 [revised]
PHST- 2017/11/24 00:00 [accepted]
PHST- 2017/12/06 06:00 [pubmed]
PHST- 2018/09/12 06:00 [medline]
PHST- 2017/12/06 06:00 [entrez]
AID - S0166-4328(17)31378-5 [pii]
AID - 10.1016/j.bbr.2017.11.034 [doi]
PST - ppublish
SO  - Behav Brain Res. 2018 Jul 2;346:96-104. doi: 10.1016/j.bbr.2017.11.034. Epub 2017 
      Dec 2.