PMID- 29207056
OWN - NLM
STAT- MEDLINE
DCOM- 20180717
LR  - 20211204
IS  - 1791-244X (Electronic)
IS  - 1107-3756 (Linking)
VI  - 41
IP  - 2
DP  - 2018 Feb
TI  - Different roles of Akt and mechanistic target of rapamycin in serum‑dependent 
      chondroprotection of human osteoarthritic chondrocytes.
PG  - 977-984
LID - 10.3892/ijmm.2017.3285 [doi]
AB  - Despite various animal serums being used widely to culture chondrocytes, the 
      regulatory mechanism of serum on chondrocyte activities has not been elucidated. 
      In the present study, human osteoarthritis (OA) chondrocytes were used to perform 
      in vitro investigations on the effect of different concentrations of bovine fetal 
      serum on extracellular matrix synthesis, cell proliferation and autophagy using 
      the Cell Counting Kit‑8 analysis, a laser‑scanning confocal microscope, and 
      western blot analysis. The results demonstrated that 5% serum exerted a 
      chondroprotective effect more than the other concentrations of serum, as it 
      simultaneously promoted cell proliferation, autophagy, and ECM synthesis in human 
      OA chondrocytes. Furthermore, the decreased mechanistic target of rapamycin 
      (mTOR) and increased Akt were observed in 5% serum‑treated OA chondrocytes. 
      Either mTOR or Akt inhibitor influenced the effect of 5% serum on cell 
      proliferation and autophagy in human OA chondrocytes, which was associated with 
      LC‑3B or B‑cell lymphoma-2 (Bcl‑2) signal molecules. Consistent with previous 
      studies, the present study proposes that 5% serum promotes cell proliferation via 
      the Akt/Bcl‑2 axis and induces autophagy via the mTOR/LC‑3B axis in human OA 
      chondrocytes. Furthermore, the different roles of Akt and mTOR in the cell 
      processes of human OA chondrocytes require consideration for preclinical and 
      clinical therapy of OA.
FAU - Zhang, Tongen
AU  - Zhang T
AD  - Department of Sports Medicine and Joint Surgery, Zhongshan Hospital, Xiamen 
      University, Xiamen, Fujian 361004, P.R. China.
FAU - Liu, Jijie
AU  - Liu J
AD  - Department of Sports Medicine and Joint Surgery, Zhongshan Hospital, Xiamen 
      University, Xiamen, Fujian 361004, P.R. China.
FAU - Zheng, Xinpeng
AU  - Zheng X
AD  - Department of Sports Medicine and Joint Surgery, Zhongshan Hospital, Xiamen 
      University, Xiamen, Fujian 361004, P.R. China.
FAU - Zhang, Bing
AU  - Zhang B
AD  - Medical School, Xiamen University, Xiamen, Fujian 361102, P.R. China.
FAU - Xia, Chun
AU  - Xia C
AD  - Department of Sports Medicine and Joint Surgery, Zhongshan Hospital, Xiamen 
      University, Xiamen, Fujian 361004, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171124
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Autophagy/drug effects
MH  - Cartilage, Articular/drug effects/*growth & development/pathology
MH  - Cattle
MH  - Cell Proliferation/drug effects
MH  - Cell Survival
MH  - Cells, Cultured/*drug effects
MH  - Chondrocytes/drug effects/pathology
MH  - Extracellular Matrix/drug effects/genetics
MH  - Humans
MH  - Osteoarthritis/*genetics/pathology
MH  - Proto-Oncogene Proteins c-akt/genetics
MH  - Serum/*chemistry
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/genetics
EDAT- 2017/12/06 06:00
MHDA- 2018/07/18 06:00
CRDT- 2017/12/06 06:00
PHST- 2017/03/24 00:00 [received]
PHST- 2017/11/22 00:00 [accepted]
PHST- 2017/12/06 06:00 [pubmed]
PHST- 2018/07/18 06:00 [medline]
PHST- 2017/12/06 06:00 [entrez]
AID - 10.3892/ijmm.2017.3285 [doi]
PST - ppublish
SO  - Int J Mol Med. 2018 Feb;41(2):977-984. doi: 10.3892/ijmm.2017.3285. Epub 2017 Nov 
      24.