PMID- 29207082
OWN - NLM
STAT- MEDLINE
DCOM- 20180718
LR  - 20180718
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 17
IP  - 2
DP  - 2018 Feb
TI  - Autophagy regulates proliferation and biliary differentiation of hepatic oval 
      cells via the MAPK/ERK signaling pathway.
PG  - 2565-2571
LID - 10.3892/mmr.2017.8157 [doi]
AB  - Hepatic oval cells (HOCs) are thought to possess self‑renewal ability and a 
      bipotential capacity for differentiation, which allows them to differentiate into 
      hepatocytes and cholangiocytes. Autophagy serves an important role in 
      self‑renewal and differentiation of stem cells; however, how autophagy 
      contributes to proliferation and differentiation of hepatic progenitor cells has 
      yet to be elucidated. In the present study, autophagy was regulated by rapamycin 
      (Rapa) and chloroquine (Chlo) administration. The results demonstrated that 
      Chlo‑treated HOCs exhibited decreased autophagic activity alongside a decreased 
      tendency to proliferate, as determined by Cell Counting Kit‑8. In addition, 
      activation of autophagy by Rapa enhanced the biliary differentiation of HOCs. 
      Furthermore, increased phosphorylated (p)‑extracellular signal‑regulated kinase 
      (ERK)/p‑p38 expression was observed following the induction of autophagy, thus 
      indicating that the mitogen‑activated protein kinase (MAPK)/ERK signaling pathway 
      was activated by autophagy to exert effects on the stimulation of HOC 
      proliferation and differentiation. In conclusion, the present study demonstrated 
      that autophagy regulates proliferation and biliary differentiation of HOCs via 
      the MAPK/ERK signaling pathway. These results suggest a role for autophagy in 
      stimulating the proliferation and differentiation of HOCs.
FAU - Chen, Dongdong
AU  - Chen D
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Wu, Xiaoxiao
AU  - Wu X
AD  - Department of Intensive Care Unit, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Zheng, Jie
AU  - Zheng J
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Dai, Ruijie
AU  - Dai R
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Mo, Zhichao
AU  - Mo Z
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Munir, Fahad
AU  - Munir F
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Ni, Xiaolong
AU  - Ni X
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Shan, Yunfeng
AU  - Shan Y
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou 
      Medical University, Wenzhou, Zhejiang 325000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20171127
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
SB  - IM
MH  - *Autophagy/drug effects
MH  - *Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Hepatocytes/*cytology/drug effects/*metabolism
MH  - *MAP Kinase Signaling System
EDAT- 2017/12/06 06:00
MHDA- 2018/07/19 06:00
CRDT- 2017/12/06 06:00
PHST- 2016/11/07 00:00 [received]
PHST- 2017/07/07 00:00 [accepted]
PHST- 2017/12/06 06:00 [pubmed]
PHST- 2018/07/19 06:00 [medline]
PHST- 2017/12/06 06:00 [entrez]
AID - 10.3892/mmr.2017.8157 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Feb;17(2):2565-2571. doi: 10.3892/mmr.2017.8157. Epub 2017 Nov 
      27.