PMID- 29208210
OWN - NLM
STAT- MEDLINE
DCOM- 20180427
LR  - 20220410
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 230
DP  - 2017 Dec
TI  - Evaluation of the delta neutrophil index from an automated blood cell analyser in 
      septic dogs.
PG  - 13-19
LID - S1090-0233(17)30206-X [pii]
LID - 10.1016/j.tvjl.2017.11.002 [doi]
AB  - Immature granulocytes (IG) are a marker of severe inflammatory states in human 
      beings and animals, and have been linked to a diagnosis of sepsis and poor 
      prognosis. The delta neutrophil index (DNI), automatically calculated by a 
      haematological analyser, provides an estimate of circulating IG. In particular, 
      an increased DNI value has been associated with the severity of sepsis, and 
      mortality, in critically ill human beings. The aims of this study were to 
      determine the DNI reference interval (RI) in healthy dogs, and to evaluate its 
      diagnostic and prognostic significance in dogs with sepsis. A total of 118 dogs 
      with sepsis undergoing a complete blood cell count (CBC) at the time of hospital 
      admission were included retrospectively. Dogs with sepsis were compared to 20 
      dogs with primary immune-mediated haemolytic anaemia (IMHA) and 99 healthy 
      controls. The DNI RI was set from 0 to 9.2%. The DNI was significantly higher in 
      dogs with sepsis compared to dogs with IMHA and healthy dogs (P<0.001), and 
      significantly higher in dogs with septic shock compared to septic dogs without 
      circulatory failure (P<0.03). No differences were detected between survivors 
      (78/118) and non-survivors (40/118). Septic dogs with a DNI above the RI had 
      significantly higher frequencies of IG and toxic neutrophil changes on manual 
      blood smear evaluation (P=0.03 and P<0.001, respectively). The DNI had a fair 
      performance in identifying dogs with sepsis in this population and predicted 
      septic shock. Larger prospective studies are needed to validate DNI measurement 
      in dogs and to test its clinical utility.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Troia, R
AU  - Troia R
AD  - Department of Veterinary Medical Science, Alma Mater Studiorum, University of 
      Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy. 
      Electronic address: roberta.troia2@unibo.it.
FAU - Agnoli, C
AU  - Agnoli C
AD  - Department of Veterinary Medical Science, Alma Mater Studiorum, University of 
      Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
FAU - Calipa, S
AU  - Calipa S
AD  - Department of Veterinary Medical Science, Alma Mater Studiorum, University of 
      Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
FAU - Segalina, S
AU  - Segalina S
AD  - Department of Veterinary Medical Science, Alma Mater Studiorum, University of 
      Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
FAU - Murgia, E
AU  - Murgia E
AD  - Department of Veterinary Medical Science, Alma Mater Studiorum, University of 
      Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
FAU - Gruarin, M
AU  - Gruarin M
AD  - Department of Veterinary Medical Science, Alma Mater Studiorum, University of 
      Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
FAU - Dondi, F
AU  - Dondi F
AD  - Department of Veterinary Medical Science, Alma Mater Studiorum, University of 
      Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
FAU - Giunti, M
AU  - Giunti M
AD  - Department of Veterinary Medical Science, Alma Mater Studiorum, University of 
      Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell'Emilia, Bologna, Italy.
LA  - eng
PT  - Journal Article
DEP - 20171108
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
SB  - IM
MH  - Animals
MH  - Autoanalysis/instrumentation/*veterinary
MH  - Case-Control Studies
MH  - Dog Diseases/*blood
MH  - Dogs
MH  - Female
MH  - Male
MH  - Neutrophils/*pathology
MH  - Reproducibility of Results
MH  - Retrospective Studies
MH  - Sepsis/blood/*veterinary
OTO - NOTNLM
OT  - Canine
OT  - Delta neutrophil index
OT  - Immature granulocytes
OT  - Sepsis
EDAT- 2017/12/07 06:00
MHDA- 2018/04/28 06:00
CRDT- 2017/12/07 06:00
PHST- 2017/02/07 00:00 [received]
PHST- 2017/07/05 00:00 [revised]
PHST- 2017/11/05 00:00 [accepted]
PHST- 2017/12/07 06:00 [entrez]
PHST- 2017/12/07 06:00 [pubmed]
PHST- 2018/04/28 06:00 [medline]
AID - S1090-0233(17)30206-X [pii]
AID - 10.1016/j.tvjl.2017.11.002 [doi]
PST - ppublish
SO  - Vet J. 2017 Dec;230:13-19. doi: 10.1016/j.tvjl.2017.11.002. Epub 2017 Nov 8.