PMID- 29208559
OWN - NLM
STAT- MEDLINE
DCOM- 20180801
LR  - 20180801
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 108
DP  - 2018 Mar
TI  - Pilose antler peptide attenuates LPS-induced inflammatory reaction.
PG  - 272-276
LID - S0141-8130(17)32488-1 [pii]
LID - 10.1016/j.ijbiomac.2017.11.176 [doi]
AB  - The present study was designed to study the effects of pilose antler peptide 
      (PAP) on primary culture of nucleus pulposus cells in intervertebral disc. We 
      demonstrated that PAP significantly inhibited lipopolysaccharides (LPS) induced 
      over-production of inflammatory factors including interleukin-1beta (IL-1beta), tumor 
      necrosis Factor-alpha (TNF-alpha) and interleukin-6 (IL-6) in nucleus pulposus cells. PAP 
      also attenuated increase of malondialdehyde (MDA) and decrease of superoxide 
      dismutase (SOD) induced by LPS challenge in a concentration-dependent manner. 
      Moreover, the expression of the protein levels of mitogen-activated protein 
      kinase (MAPK)/nuclear transcription factor-kappaB(NF-kappaB) were increased accompanying 
      with the LPS challenge, which were significantly reversed after PAP treatment. 
      Our results demonstrated the ability of PAP to antagonize LPS-mediated 
      inflammation in primary culture of nucleus pulposus in intervertebral disc, 
      suggesting a beneficial potential for its clinical application.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Dong, Yu
AU  - Dong Y
AD  - Center for Translational Systems Biology and Neuroscience, School of Basic 
      Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, 
      China.
FAU - Liu, Li
AU  - Liu L
AD  - School of Pharmacy, Guangdong Medical University, Dongguan 523808, China.
FAU - Shan, Xin
AU  - Shan X
AD  - Center for Translational Systems Biology and Neuroscience, School of Basic 
      Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, 
      China.
FAU - Tang, Juanjuan
AU  - Tang J
AD  - Center for Translational Systems Biology and Neuroscience, School of Basic 
      Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, 
      China.
FAU - Xia, Baomei
AU  - Xia B
AD  - Faculty of Rehabilitation Science, Nanjing Normal University of Special 
      Education, Nanjing, 210023, China.
FAU - Cheng, Xiaolan
AU  - Cheng X
AD  - Laboratory of Cellular and Molecular Biology, Jiangsu Province Academy of 
      Traditional Chinese Medicine, Nanjing 210028, China.
FAU - Chen, Yanyan
AU  - Chen Y
AD  - Center for Translational Systems Biology and Neuroscience, School of Basic 
      Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, 
      China.
FAU - Tao, Weiwei
AU  - Tao W
AD  - Center for Translational Systems Biology and Neuroscience, School of Basic 
      Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, 
      China. Electronic address: taoweiweinanjing@126.com.
LA  - eng
PT  - Journal Article
DEP - 20171205
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Peptides)
RN  - 0 (pilose antler peptide)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Survival/drug effects
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Inflammation/drug therapy/*etiology/*metabolism/pathology
MH  - Inflammation Mediators/metabolism
MH  - Lipopolysaccharides/*adverse effects
MH  - Malondialdehyde/metabolism
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - NF-kappa B/metabolism
MH  - Peptides/*pharmacology
MH  - Rats
MH  - Signal Transduction/drug effects
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - Intervertebral disc degeneration
OT  - MAPK
OT  - NF-kappaB
OT  - Pilose antler peptide
EDAT- 2017/12/07 06:00
MHDA- 2018/08/02 06:00
CRDT- 2017/12/07 06:00
PHST- 2017/07/09 00:00 [received]
PHST- 2017/11/26 00:00 [revised]
PHST- 2017/11/29 00:00 [accepted]
PHST- 2017/12/07 06:00 [pubmed]
PHST- 2018/08/02 06:00 [medline]
PHST- 2017/12/07 06:00 [entrez]
AID - S0141-8130(17)32488-1 [pii]
AID - 10.1016/j.ijbiomac.2017.11.176 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2018 Mar;108:272-276. doi: 10.1016/j.ijbiomac.2017.11.176. 
      Epub 2017 Dec 5.