PMID- 29214002
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220311
IS  - 2008-3289 (Print)
IS  - 2008-4447 (Electronic)
IS  - 2008-3289 (Linking)
VI  - 9
IP  - 2
DP  - 2017 Apr
TI  - Antibacterial activity of poly-l-arginine under different conditions.
PG  - 103-111
AB  - BACKGROUND AND OBJECTIVES: Arginine-rich peptides are an important class of 
      antimicrobial peptides (AMPs) that exert their antibacterial activity via a lytic 
      mechanism. Although the antibacterial activity of arginine-rich peptides has been 
      already evaluated, no reports have so far been evaluated the influence of 
      reaction conditions on their antimicrobial potential. The aim of the present 
      study was to investigate the influence of pH, temperature, and glycine on 
      antibacterial activity of poly-l-arginine (PLA) with a molecular weight of 5-15 
      kDa against Escherichia coli O157:H7 and Staphylococcus aureus. MATERIALS AND 
      METHODS: The percentage of growth inhibition of PLA against both bacteria was 
      analyzed at various pH, temperatures and sub-inhibitory concentrations of glycine 
      by two-fold broth microdilution method. RESULTS: The results showed that PLA had 
      antibacterial activity against E. coli O157:H7 and S. aureus and the inhibitory 
      effect increased with increasing PLA concentration. The antimicrobial activity of 
      PLA against both microorganisms was higher in basic media than under acidic or 
      neutral conditions. At 1/2 times the MIC, heat treatment intensified the toxicity 
      of PLA against E. coli O157:H7 whereas the susceptibility to PLA seems to be 
      temperature independent for S. aureus. The MICs of glycine against E. coli 
      O157:H7 and S. aureus were 12.5 and 25 mg ml(-1), respectively. The antibacterial 
      activity of PLA against both microorganisms increased, as indicated by the 
      increasing growth inhibition percentage of this peptide with increasing glycine 
      concentration. CONCLUSION: The antibacterial activity of PLA against S. aureus 
      and E. coli O157:H7 depends on pH and glycine concentration.
FAU - Sepahi, Mohaddeseh
AU  - Sepahi M
AD  - Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, 
      Mashhad, Iran.
FAU - Jalal, Razieh
AU  - Jalal R
AD  - Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, 
      Mashhad, Iran.
AD  - Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, 
      Ferdowsi University of Mashhad, Mashhad, Iran.
FAU - Mashreghi, Mansour
AU  - Mashreghi M
AD  - Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, 
      Mashhad, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Microbiol
JT  - Iranian journal of microbiology
JID - 101518404
PMC - PMC5715275
OTO - NOTNLM
OT  - Antibacterial activity
OT  - Escherichia coli O157:H7
OT  - Glycine
OT  - Poly-l-arginine
OT  - Staphylococcus aureus
EDAT- 2017/12/08 06:00
MHDA- 2017/12/08 06:01
PMCR- 2017/04/01
CRDT- 2017/12/08 06:00
PHST- 2017/12/08 06:00 [entrez]
PHST- 2017/12/08 06:00 [pubmed]
PHST- 2017/12/08 06:01 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - ijm-9-103 [pii]
PST - ppublish
SO  - Iran J Microbiol. 2017 Apr;9(2):103-111.