PMID- 29214487
OWN - NLM
STAT- MEDLINE
DCOM- 20180607
LR  - 20211204
IS  - 1976-3794 (Electronic)
IS  - 1225-8873 (Linking)
VI  - 55
IP  - 12
DP  - 2017 Dec
TI  - Effects of dietary poly-beta-hydroxybutyrate (PHB) on microbiota composition and the 
      mTOR signaling pathway in the intestines of litopenaeus vannamei.
PG  - 946-954
LID - 10.1007/s12275-017-7273-y [doi]
AB  - Poly-beta-hydroxybutyrate (PHB) is a natural polymer of the short chain fatty acid 
      beta-hydroxybutyrate, which acts as a microbial control agent. The mammalian target 
      of the rapamycin (mTOR) signaling pathway plays a crucial role in intestine 
      inflammation and epithelial morphogenesis. In this study, we examined the 
      composition of intestine microbiota, and mTOR signaling-related gene expression 
      in Pacific white shrimp Litopenaeus vannamei fed diets containing different 
      levels of PHB: 0% (Control), 1% (PHB1), 3% (PHB3), and 5% (PHB5) (w/w) for 35 
      days. High-throughput sequencing analysis revealed that dietary PHB altered the 
      composition and diversity of intestine microbiota, and that the microbiota 
      diversity decreased with the increasing doses of PHB. Specifically, dietary PHB 
      increased the relative abundance of Proteobacteria and Tenericutes in the PHB1 
      and PHB5 groups, respectively, and increased that of Gammaproteobacteria in the 
      three PHB groups. Alternatively, PHB decreased Alphaproteobacteria in the PHB3 
      and PHB5 groups. At the genus level, dietary PHB increased the abundance of 
      beneficial bacteria, such as Bacillus, Lactobacillus, Lactococcus, Clostridium, 
      and Bdellovibrio. The relative mRNA expression levels of the mTOR 
      signaling-related genes TOR, 4E-BP, eIF4E1alpha, and eIF4E2 all increased in the 
      three PHB treatment groups. These results revealed that dietary PHB 
      supplementation had a beneficial effect on intestine health of L. vannamei by 
      modulating the composition of intestine microbiota and activating mTOR signaling.
FAU - Duan, Yafei
AU  - Duan Y
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, 
      Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Guangzhou, 510300, P. R. China.
FAU - Zhang, Yue
AU  - Zhang Y
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, 
      Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Guangzhou, 510300, P. R. China.
FAU - Dong, Hongbiao
AU  - Dong H
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, 
      Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Guangzhou, 510300, P. R. China.
FAU - Wang, Yun
AU  - Wang Y
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, 
      Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Guangzhou, 510300, P. R. China.
FAU - Zhang, Jiasong
AU  - Zhang J
AD  - Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, 
      Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese 
      Academy of Fishery Sciences, Guangzhou, 510300, P. R. China. 
      jiasongzhang@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20171207
PL  - Korea (South)
TA  - J Microbiol
JT  - Journal of microbiology (Seoul, Korea)
JID - 9703165
RN  - 0 (Arthropod Proteins)
RN  - 0 (Eukaryotic Initiation Factor-4E)
RN  - 0 (Food Additives)
RN  - 0 (Hydroxybutyrates)
RN  - 0 (Polyesters)
RN  - 26063-00-3 (poly-beta-hydroxybutyrate)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animal Feed/analysis
MH  - Animals
MH  - Arthropod Proteins/genetics/metabolism
MH  - Eukaryotic Initiation Factor-4E/genetics/metabolism
MH  - Food Additives/metabolism
MH  - *Gastrointestinal Microbiome
MH  - Hydroxybutyrates/*metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/microbiology
MH  - Penaeidae/genetics/*metabolism/*microbiology
MH  - Polyesters/*metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
OTO - NOTNLM
OT  - Litopenaeus vannamei
OT  - intestine microbiota
OT  - mTOR signaling
OT  - poly-beta-hydroxybutyrate (PHB)
OT  - prebiotic
EDAT- 2017/12/08 06:00
MHDA- 2018/06/08 06:00
CRDT- 2017/12/08 06:00
PHST- 2017/06/22 00:00 [received]
PHST- 2017/11/01 00:00 [accepted]
PHST- 2017/09/23 00:00 [revised]
PHST- 2017/12/08 06:00 [entrez]
PHST- 2017/12/08 06:00 [pubmed]
PHST- 2018/06/08 06:00 [medline]
AID - 10.1007/s12275-017-7273-y [pii]
AID - 10.1007/s12275-017-7273-y [doi]
PST - ppublish
SO  - J Microbiol. 2017 Dec;55(12):946-954. doi: 10.1007/s12275-017-7273-y. Epub 2017 
      Dec 7.