PMID- 29215706
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20190415
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 233
IP  - 6
DP  - 2018 Jun
TI  - Map and correlate intracellular calcium response and matrix deposition in 
      cartilage under physiological oxygen tensions.
PG  - 4949-4960
LID - 10.1002/jcp.26326 [doi]
AB  - Face to the limited repair capability of cartilage, we intended to find out 
      signaling responsible for its matrix synthesis. Since spontaneous calcium 
      response likes a label of cell status, here it was mapped in fresh and 24 hr 
      cultured in situ chondrocytes under oxygen tensions of 20%, 5%, and 1% as well as 
      mimic hypoxia conditions. The calcium source was traced using ethylene glycol-bis 
      (beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) and thapsigargin (TG) to 
      treat cartilage. Their relative matrix of type II collagen (COLL-II) and 
      glycosaminoglycan (GAG) were quantified after cultured for 3 and 7 days. We 
      disclosed the specific fingerprint of calcium response and matrix deposition 
      along the histological zones under various oxygen tensions, from which the 
      effects of hyperoxia, normoxia, and hypoxia conditions on as well as the optimal 
      oxygen tensions for maintenance of various zones of cartilage or chondrocytes 
      were derived and obtained. Our results revealed that cytoplasm calcium was 
      conducive to synthesize COLL-II but detrimental to synthesize GAG. These results 
      provide correlation in addition to details of intracellular calcium response and 
      matrix deposition in in situ cartilage along its histological zones under 
      physiological oxygen tensions.
CI  - (c) 2017 Wiley Periodicals, Inc.
FAU - Zhou, Jin
AU  - Zhou J
AD  - Key Laboratory for Biorheological Science and Technology of Ministry of 
      Education, State and Local Joint Engineering Laboratory for Vascular Implants, 
      College of Bioengineering, Chongqing University, Chongqing, China.
FAU - Yue, Danyang
AU  - Yue D
AD  - Key Laboratory for Biorheological Science and Technology of Ministry of 
      Education, State and Local Joint Engineering Laboratory for Vascular Implants, 
      College of Bioengineering, Chongqing University, Chongqing, China.
FAU - Bai, Yuying
AU  - Bai Y
AD  - Key Laboratory for Biorheological Science and Technology of Ministry of 
      Education, State and Local Joint Engineering Laboratory for Vascular Implants, 
      College of Bioengineering, Chongqing University, Chongqing, China.
FAU - Kong, Fei
AU  - Kong F
AD  - Key Laboratory for Biorheological Science and Technology of Ministry of 
      Education, State and Local Joint Engineering Laboratory for Vascular Implants, 
      College of Bioengineering, Chongqing University, Chongqing, China.
FAU - Pan, Jun
AU  - Pan J
AUID- ORCID: 0000-0002-5091-982X
AD  - Key Laboratory for Biorheological Science and Technology of Ministry of 
      Education, State and Local Joint Engineering Laboratory for Vascular Implants, 
      College of Bioengineering, Chongqing University, Chongqing, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180104
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Collagen Type II)
RN  - 0 (Glycosaminoglycans)
RN  - S88TT14065 (Oxygen)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Cartilage, Articular/*metabolism/pathology
MH  - Cell Hypoxia
MH  - *Cellular Microenvironment
MH  - Chondrocytes/*metabolism/pathology
MH  - *Chondrogenesis
MH  - Collagen Type II/metabolism
MH  - Extracellular Matrix/*metabolism/pathology
MH  - Glycosaminoglycans/metabolism
MH  - Oxygen/*metabolism
MH  - Signal Transduction
MH  - Sus scrofa
MH  - Time Factors
OTO - NOTNLM
OT  - GAG
OT  - collage-II
OT  - histological zone
OT  - hyperoxia
OT  - hypoxia
EDAT- 2017/12/08 06:00
MHDA- 2019/04/16 06:00
CRDT- 2017/12/08 06:00
PHST- 2017/09/27 00:00 [received]
PHST- 2017/11/29 00:00 [accepted]
PHST- 2017/12/08 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2017/12/08 06:00 [entrez]
AID - 10.1002/jcp.26326 [doi]
PST - ppublish
SO  - J Cell Physiol. 2018 Jun;233(6):4949-4960. doi: 10.1002/jcp.26326. Epub 2018 Jan 
      4.