PMID- 29217339
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 0975-9476 (Print)
IS  - 0976-2809 (Electronic)
IS  - 0975-9476 (Linking)
VI  - 10
IP  - 2
DP  - 2019 Apr-Jun
TI  - Standardization, anti-carcinogenic potential and biosafety of Indian propolis.
PG  - 81-87
LID - S0975-9476(17)30185-7 [pii]
LID - 10.1016/j.jaim.2017.06.003 [doi]
AB  - BACKGROUND: Propolis from apiculture is known for wide range of medicinal 
      properties owing to its vast chemical constituents including polyphenols, 
      flavonoids and anticancer agent Caffeic acid phenethyl ester (CAPE). OBJECTIVES: 
      The objective of the study was to extract and standardize Indian propolis (IP) 
      with respect to selected markers by newly developed High performance liquid 
      chromatography (HPLC) method, to evaluate in vitro and in vivo anticancer 
      activity and biosafety of Indian propolis. MATERIALS AND METHODS: IP was 
      extracted, optimized and standardized using a newly developed and validated HPLC 
      method for simultaneous estimation of caffeic acid, apigenin, quercetin and CAPE. 
      The standardised ethanolic extract of IP (EEIP) was screened for in vitro 
      cytotoxicity using sulforhodamine B (SRB) assay, in vivo anti-carcinogenic effect 
      against Dalton's Lymphoma ascites (DLA) cells, hemolytic effect and pesticide 
      analysis. RESULTS: The EEIP was found to contain more amount of total flavonoids 
      (23.61 +/- 0.0452 mg equivalent of quercetin/g), total polyphenolics 
      (34.82 +/- 0.0785 mg equivalent of gallic acid/g) and all selected markers except 
      caffeic acid compared to all other extracts. EEIP showed better anti-cancer 
      potential than CAPE on MCF-7 and HT-29 cell line and significant (p < 0.01) in 
      vivo anti-carcinogenic effects against DLA in comparison with 5-fluorouracil. 
      EEIP was found to be non-hemolytic. CONCLUSION: From in vitro cytotoxicity, in 
      vivo anti-carcinogenicity and biosafety studies it can be concluded that the 
      standardized EEIP is safe and can be considered for further development as a 
      biomedicine.
CI  - Copyright (c) 2017 Transdisciplinary University, Bangalore and World Ayurveda 
      Foundation. Published by Elsevier B.V. All rights reserved.
FAU - Kapare, Harshad
AU  - Kapare H
AD  - Department of Quality Assurance Techniques, Poona College of Pharmacy, Bharati 
      Vidyapeeth University, Erandwane, Pune 411038, India.
FAU - Lohidasan, Sathiyanarayanan
AU  - Lohidasan S
AD  - Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati 
      Vidyapeeth University, Erandwane, Pune 411038, India. Electronic address: 
      pharmsathiya@gmail.com.
FAU - Sinnathambi, Arulmozhi
AU  - Sinnathambi A
AD  - Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth 
      University, Erandwane, Pune 411038, India.
FAU - Mahadik, Kakasaheb
AU  - Mahadik K
AD  - Department of Pharmaceutical Chemistry, Poona College of Pharmacy, Bharati 
      Vidyapeeth University, Erandwane, Pune 411038, India.
LA  - eng
PT  - Journal Article
DEP - 20171206
PL  - United States
TA  - J Ayurveda Integr Med
JT  - Journal of Ayurveda and integrative medicine
JID - 101551404
PMC - PMC6598798
OTO - NOTNLM
OT  - Anticancer
OT  - Caffeic acid phenethyl ester
OT  - HPLC
OT  - Indian propolis
EDAT- 2017/12/09 06:00
MHDA- 2017/12/09 06:01
PMCR- 2017/12/06
CRDT- 2017/12/09 06:00
PHST- 2017/03/30 00:00 [received]
PHST- 2017/05/19 00:00 [revised]
PHST- 2017/06/01 00:00 [accepted]
PHST- 2017/12/09 06:00 [pubmed]
PHST- 2017/12/09 06:01 [medline]
PHST- 2017/12/09 06:00 [entrez]
PHST- 2017/12/06 00:00 [pmc-release]
AID - S0975-9476(17)30185-7 [pii]
AID - 10.1016/j.jaim.2017.06.003 [doi]
PST - ppublish
SO  - J Ayurveda Integr Med. 2019 Apr-Jun;10(2):81-87. doi: 10.1016/j.jaim.2017.06.003. 
      Epub 2017 Dec 6.