PMID- 29218086
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 9
IP  - 11
DP  - 2017
TI  - MicroRNA-520d-5p inhibits human glioma cell proliferation and induces cell cycle 
      arrest by directly targeting PTTG1.
PG  - 4872-4887
AB  - Glioma accounts for the majority of primary malignant brain tumors in adults and 
      is highly aggressive. Although various therapeutic approaches have been applied, 
      outcomes of glioma treatment remain poor. Acquiring a better understanding of the 
      pathogenic mechanisms is essential to the design of effective therapeutic 
      strategies. Previous studies have found that miR-520d-5p was negatively 
      correlated with glioma grade, but its role and mechanism in glioma progression 
      remain largely unknown. In the present study, we reported that miR-520d-5p 
      directly targeted the Pituitary Tumor Transforming Gene 1 (PTTG1) and functioned 
      as a tumor-suppressor in glioma. The expression of miR-520d-5p in glioma cells 
      and specimens were detected by Quantitative reverse transcription-PCR and 
      Fluorescence in situ hybridization (FISH). The effects of miR-520d-5p on glioma 
      progression was examined by cell-counting kit 8, colony formation, 
      5-ethynyl-2-deoxyuridine (EDU) and flow cytometry assays. Using bioinformatics 
      and luciferase reporter assays, we identified PTTG1 as a novel and direct target 
      of miR-520d-3p. A xenograft model was used to study the effect of miR-520d-5p on 
      tumor growth and angiogenesis. We found that miR-520d-5p expression was 
      significantly decreased in glioma cell lines and tissues. Overexpression of 
      miR-520d-5p showed a significant inhibitory effect on cell proliferation and 
      accompanied cell cycle G0/G1 arrest in U87-MG and LN229 glioma cells. PTTG1 was a 
      novel and direct target of miR-520d-5p, and the protein expression of PTTG1 was 
      markedly reduced after overexpression of miR-520d-5p in U87-MG and LN229 cells. 
      Overexpression of PTTG1 reversed the inhibitory effect of miR-520d-5p on glioma 
      cell proliferation. In vivo studies confirmed that miR-520d-5p overexpression 
      retarded the growth of U87 xenograft tumors, which was accompanied by reduced 
      expression of PTTG1. In conclusion, these results provide compelling evidence 
      that miR-520d-5p functions as an anti-onco-miRNA, which is important in 
      inhibiting cell proliferation in GBM, and its anti-oncogenic effects are mediated 
      chiefly through direct suppression of PTTG1 expression. Therefore, we suggest 
      that miR-520d-5p is a potential candidate for the prevention of glioblastoma.
FAU - Zhi, Tongle
AU  - Zhi T
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Jiang, Kuan
AU  - Jiang K
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
AD  - Department of Neurosurgery, Yixing People's HospitalYixing 214200, Jiangsu 
      Province, China.
FAU - Xu, Xiupeng
AU  - Xu X
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Yu, Tianfu
AU  - Yu T
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Wu, Weining
AU  - Wu W
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Nie, Er
AU  - Nie E
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Zhou, Xu
AU  - Zhou X
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Jin, Xin
AU  - Jin X
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Zhang, Junxia
AU  - Zhang J
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Wang, Yingyi
AU  - Wang Y
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
FAU - Liu, Ning
AU  - Liu N
AD  - Department of Neurosurgery, The First Affiliated Hospital of Nanjing Medical 
      UniversityNanjing 210029, Jiangsu Province, China.
LA  - eng
PT  - Journal Article
DEP - 20171115
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC5714772
OTO - NOTNLM
OT  - PTTG1
OT  - glioma
OT  - miR-520d-5p
OT  - proliferation
COIS- None.
EDAT- 2017/12/09 06:00
MHDA- 2017/12/09 06:01
PMCR- 2017/11/15
CRDT- 2017/12/09 06:00
PHST- 2017/08/01 00:00 [received]
PHST- 2017/10/10 00:00 [accepted]
PHST- 2017/12/09 06:00 [entrez]
PHST- 2017/12/09 06:00 [pubmed]
PHST- 2017/12/09 06:01 [medline]
PHST- 2017/11/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2017 Nov 15;9(11):4872-4887. eCollection 2017.