PMID- 29219038
OWN - NLM
STAT- MEDLINE
DCOM- 20190417
LR  - 20190417
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 34
IP  - 6
DP  - 2017 Dec
TI  - Duplication and Variation in the Major Histocompatibility Complex Genes in 
      Blakiston's Fish Owl, Bubo blakistoni.
PG  - 484-489
LID - 10.2108/zs170039 [doi]
AB  - The major histocompatibility complex (MHC) includes many genes that are essential 
      for the adaptive immune system, and variation in the antigen binding site (ABS) 
      is related to resistance against pathogens. In the present study, quantitative 
      real-time PCR indicated a larger number of MHC gene copies in the endangered 
      population of Blakiston's fish owl (Bubo blakistoni) than in five other owl 
      species, and massively parallel pyrosequencing detected more MHC class IIbeta per 
      individual alleles in B. blakistoni than in the other species. A chromosomal 
      fluorescence in situ hybridization (FISH) analysis showed that the MHC class I 
      and class IIbeta loci are closely linked on a single pair of microchromosomes, 
      indicating that the MHC genes were tandemly duplicated in a limited chromosomal 
      region. Because B. blakistoni has twice as many MHC genes as its sister species, 
      the tawny fish owl (Bubo flavipes), the duplication of MHC genes occurred after 
      these species diverged by speciation. A Bayesian molecular phylogenetic analysis 
      showed that the DAB1 and DAB2 lineages of MHC class IIbeta alleles from various 
      strigid species each formed a separate clade, indicating that the two allelic 
      lineages preceded the radiation of Strigidae and evolved as paralogs. By 
      contrast, the ABS sequences did not form distinct clades between DAB1 and DAB2 
      alleles but were intermixed, presumably due to gene conversion. Despite the low 
      diversity of alleles per locus, B. blakistoni had many lineages of MHC class IIbeta 
      alleles. Gene duplication increases variation in the MHC genes in this species, 
      and could have facilitated adaptation in small populations.
FAU - Omote, Keita
AU  - Omote K
AD  - 1 Department of Natural History Sciences, Graduate School of Science, Hokkaido 
      University, Sapporo 060-0810, Japan.
FAU - Kohyama, Tetsuo I
AU  - Kohyama TI
AD  - 2 Department of Biological Sciences, Faculty of Science, Hokkaido University, 
      Sapporo 060-0810, Japan.
FAU - Nishida, Chizuko
AU  - Nishida C
AD  - 1 Department of Natural History Sciences, Graduate School of Science, Hokkaido 
      University, Sapporo 060-0810, Japan.
AD  - 2 Department of Biological Sciences, Faculty of Science, Hokkaido University, 
      Sapporo 060-0810, Japan.
FAU - Saito, Keisuke
AU  - Saito K
AD  - 3 Institute for Raptor Biomedicine, Kushiro 084-0922, Japan.
FAU - Fujimoto, Satoshi
AU  - Fujimoto S
AD  - 4 Kushiro City Zoo, Kushiro 085-0201, Japan.
FAU - Masuda, Ryuichi
AU  - Masuda R
AD  - 1 Department of Natural History Sciences, Graduate School of Science, Hokkaido 
      University, Sapporo 060-0810, Japan.
AD  - 2 Department of Biological Sciences, Faculty of Science, Hokkaido University, 
      Sapporo 060-0810, Japan.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
SB  - IM
MH  - Animals
MH  - *Gene Duplication
MH  - *Genetic Variation
MH  - Major Histocompatibility Complex/*genetics
MH  - Strigiformes/*genetics
OTO - NOTNLM
OT  - MHC class I
OT  - MHC class Iibeta
OT  - fluorescence in situ hybridization
OT  - gene duplication
OT  - quantitative real-time PCR
EDAT- 2017/12/09 06:00
MHDA- 2019/04/18 06:00
CRDT- 2017/12/09 06:00
PHST- 2017/12/09 06:00 [entrez]
PHST- 2017/12/09 06:00 [pubmed]
PHST- 2019/04/18 06:00 [medline]
AID - 10.2108/zs170039 [doi]
PST - ppublish
SO  - Zoolog Sci. 2017 Dec;34(6):484-489. doi: 10.2108/zs170039.