PMID- 29221926 OWN - NLM STAT- MEDLINE DCOM- 20180817 LR - 20180817 IS - 1873-3492 (Electronic) IS - 0009-8981 (Linking) VI - 477 DP - 2018 Feb TI - Plasma protein profiling of patients with intraductal papillary mucinous neoplasm of the pancreas as potential precursor lesions of pancreatic cancer. PG - 127-134 LID - S0009-8981(17)30493-X [pii] LID - 10.1016/j.cca.2017.12.008 [doi] AB - Efforts for the early diagnosis of the pancreatic ductal adenocarcinoma (PDAC) have recently been driven to one of the precursor lesions, namely intraductal papillary mucinous neoplasm of the pancreas (IPMN). Only a few studies have focused on IPMN molecular biology and its overall progression to cancer. Therefore, IPMN lacks comprehensive characterization which makes its clinical management controversial. In this study, we characterized plasma proteins in the presence of IPMNs in comparison to healthy controls, chronic pancreatitis, and PDAC by a proteomics approach using data-independent acquisition based mass spectrometry. We describe several protein sets that could aid IPMN diagnosis, but also differentiation of IPMN from healthy controls, as well as from benign and malignant diseases. Among all, high levels of carbonic anhydrases and hemoglobins were characteristic for the IPMN group. By employing ELISA based quantification we validated our results for human tissue inhibitor of metalloproteinase inhibitor 1 (TIMP-1). We consider IPMN management directed towards an early potential cancer development a crucial opportunity before PDAC initiation and thus its early detection and cure. CI - Copyright (c) 2017 Elsevier B.V. All rights reserved. FAU - Ilies, Maria AU - Ilies M AD - Department of Pharmaceutical Analysis, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, no. 6 Louis Pasteur st., 400349 Cluj-Napoca, Romania; Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, F.-L.-Jahn-Str. 15a, 17475 Greifswald, Germany. Electronic address: ilies.maria@umfcluj.ro. FAU - Sappa, Praveen Kumar AU - Sappa PK AD - Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, F.-L.-Jahn-Str. 15a, 17475 Greifswald, Germany. Electronic address: praveen.kumar@uni-greifswald.de. FAU - Iuga, Cristina Adela AU - Iuga CA AD - Department of Pharmaceutical Analysis, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, no. 6 Louis Pasteur st., 400349 Cluj-Napoca, Romania; Department of Proteomics and Metabolomics, MedFuture Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, no. 4-6 Louis Pasteur st., 400349 Cluj-Napoca, Romania. Electronic address: iugac@umfcluj.ro. FAU - Loghin, Felicia AU - Loghin F AD - Department of Toxicology, Faculty of Pharmacy, Iuliu Hatieganu University of Medicine and Pharmacy, no. 6 Louis Pasteur st., 400349 Cluj-Napoca, Romania. Electronic address: floghin@umfcluj.ro. FAU - Gesell Salazar, Manuela AU - Gesell Salazar M AD - Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, F.-L.-Jahn-Str. 15a, 17475 Greifswald, Germany. Electronic address: gesell@uni-greifswald.de. FAU - Weiss, Frank Ulrich AU - Weiss FU AD - Department of Medicine A, University Medicine Greifswald, Sauerbruchstr., 17475 Greifswald, Germany. Electronic address: ulrich.weiss@uni-greifswald.de. FAU - Beyer, Georg AU - Beyer G AD - Department of Medicine A, University Medicine Greifswald, Sauerbruchstr., 17475 Greifswald, Germany; Department of Medicine II, University Hospital, LMU Munich, Marchioninistr. 15, 81377 Munchen, Germany. Electronic address: georg.beyer@med.uni-muenchen.de. FAU - Lerch, Markus M AU - Lerch MM AD - Department of Medicine A, University Medicine Greifswald, Sauerbruchstr., 17475 Greifswald, Germany. Electronic address: lerch@uni-greifswald.de. FAU - Volker, Uwe AU - Volker U AD - Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, F.-L.-Jahn-Str. 15a, 17475 Greifswald, Germany. Electronic address: voelker@uni-greifswald.de. FAU - Mayerle, Julia AU - Mayerle J AD - Department of Medicine A, University Medicine Greifswald, Sauerbruchstr., 17475 Greifswald, Germany; Department of Medicine II, University Hospital, LMU Munich, Marchioninistr. 15, 81377 Munchen, Germany. Electronic address: julia.mayerle@med.uni-muenchen.de. FAU - Hammer, Elke AU - Hammer E AD - Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, F.-L.-Jahn-Str. 15a, 17475 Greifswald, Germany. Electronic address: hammer@uni-greifswald.de. LA - eng PT - Journal Article DEP - 20171206 PL - Netherlands TA - Clin Chim Acta JT - Clinica chimica acta; international journal of clinical chemistry JID - 1302422 RN - 0 (Blood Proteins) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Blood Proteins/*analysis MH - Carcinoma, Papillary/*blood/diagnosis MH - Computational Biology MH - Female MH - Humans MH - Male MH - Middle Aged MH - Pancreas/*pathology MH - Pancreatic Neoplasms/*blood/diagnosis OTO - NOTNLM OT - Data independent acquisition OT - IPMN OT - Intraductal papillary mucinous neoplasm OT - Pancreatic cancer OT - Plasma OT - Proteomics EDAT- 2017/12/10 06:00 MHDA- 2018/08/18 06:00 CRDT- 2017/12/10 06:00 PHST- 2017/10/16 00:00 [received] PHST- 2017/11/17 00:00 [revised] PHST- 2017/12/05 00:00 [accepted] PHST- 2017/12/10 06:00 [pubmed] PHST- 2018/08/18 06:00 [medline] PHST- 2017/12/10 06:00 [entrez] AID - S0009-8981(17)30493-X [pii] AID - 10.1016/j.cca.2017.12.008 [doi] PST - ppublish SO - Clin Chim Acta. 2018 Feb;477:127-134. doi: 10.1016/j.cca.2017.12.008. Epub 2017 Dec 6.