PMID- 29222846
OWN - NLM
STAT- MEDLINE
DCOM- 20191113
LR  - 20191113
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 12
IP  - 4
DP  - 2018 Apr
TI  - Influence of the three-dimensional culture of human bone marrow mesenchymal 
      stromal cells within a macroporous polysaccharides scaffold on Pannexin 1 and 
      Pannexin 3.
PG  - e1936-e1949
LID - 10.1002/term.2625 [doi]
AB  - Because cell interactions play a fundamental role for cell differentiation, we 
      investigated the expression of Pannexin 1 and Pannexin 3 in human bone marrow 
      mesenchymal stromal cells (HBMSCs) in a three-dimensional (3D) microenvironment 
      provided by a polysaccharide-based macroporous scaffold. The pannexin (Panx) 
      family consists of three members, Panx1, Panx2, and Panx3. The roles of Panx 
      large-pore ion and metabolite channels are recognized in many physiological and 
      pathophysiological scenarios, but the role of these proteins in human 
      physiological processes is still under investigation. Our study demonstrates that 
      HBMSCs cultured within 3D scaffolds have induced Panx1 and Panx3 expression, 
      compared with two-dimensional culture and that the Panx3 gene expression profile 
      correlates with those of bone markers on mesenchymal stromal cells culture into 
      the 3D scaffold. We showed that Panx1 is involved in the HBMSCs 3D cell-cell 
      organization, as acting on the size of cellular aggregates, demonstrated by the 
      use of Probenecid and the mimetic peptide 10panx1 as specific inhibitors. 
      Inhibition of Panx3 using siRNA strategy shows to reduce the expression of 
      osteocalcin as osteoblast-specific marker by HBMSCs cultured in 3D conditions, 
      suggesting a role of this Panx in osteogenesis. Moreover, we evaluated Panx1 and 
      Panx3 expression within the cellularized scaffolds upon subcutaneous implantation 
      in NOG (NOD/Shi-scid/IL-2Rgamma(null) ) mice, where we could observe a more intense 
      expression in the constructs than in the surrounding tissues in vivo. This study 
      provides new insights on the expression of pannexins in HBMSCs on a 3D 
      microenvironment during the osteogenic differentiation, in vitro and in vivo.
CI  - Copyright (c) 2017 John Wiley & Sons, Ltd.
FAU - Guerrero, Julien
AU  - Guerrero J
AD  - Inserm, U1026, Tissue Bioengineering, University of Bordeaux, Bordeaux Cedex, 
      France.
AD  - Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 
      Switzerland.
FAU - Oliveira, Hugo
AU  - Oliveira H
AD  - Inserm, U1026, Tissue Bioengineering, University of Bordeaux, Bordeaux Cedex, 
      France.
FAU - Aid, Rachida
AU  - Aid R
AD  - Inserm U1148, LVTS, X. Bichat Hospital, University Paris Diderot F-75018 Paris, 
      Institut Galilee, University Paris 13, 93430 Villetaneuse Paris Cedex 18; 
      University Paris Diderot, CHUX, Bichat, Paris, France.
FAU - Bareille, Reine
AU  - Bareille R
AD  - Inserm, U1026, Tissue Bioengineering, University of Bordeaux, Bordeaux Cedex, 
      France.
FAU - Siadous, Robin
AU  - Siadous R
AD  - Inserm, U1026, Tissue Bioengineering, University of Bordeaux, Bordeaux Cedex, 
      France.
FAU - Letourneur, Didier
AU  - Letourneur D
AD  - Inserm U1148, LVTS, X. Bichat Hospital, University Paris Diderot F-75018 Paris, 
      Institut Galilee, University Paris 13, 93430 Villetaneuse Paris Cedex 18; 
      University Paris Diderot, CHUX, Bichat, Paris, France.
FAU - Mao, Yong
AU  - Mao Y
AD  - The New Jersey Center for Biomaterials, Department of Chemistry and Chemical 
      Biology, Rutgers The State University of New Jersey, Piscataway, NJ, USA.
FAU - Kohn, Joachim
AU  - Kohn J
AD  - The New Jersey Center for Biomaterials, Department of Chemistry and Chemical 
      Biology, Rutgers The State University of New Jersey, Piscataway, NJ, USA.
FAU - Amedee, Joelle
AU  - Amedee J
AUID- ORCID: 0000-0002-4888-0129
AD  - Inserm, U1026, Tissue Bioengineering, University of Bordeaux, Bordeaux Cedex, 
      France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180108
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Connexins)
RN  - 0 (Dextrans)
RN  - 0 (Glucans)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (PANX1 protein, human)
RN  - 0 (PANX3 protein, human)
RN  - 8ZQ0AYU1TT (pullulan)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - *Cell Culture Techniques
MH  - Connexins/*biosynthesis
MH  - Dextrans/*chemistry
MH  - Glucans/*chemistry
MH  - Heterografts
MH  - Humans
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - Porosity
MH  - Tissue Scaffolds/*chemistry
OTO - NOTNLM
OT  - 3D microenvironment
OT  - Pannexin 1
OT  - Pannexin 3
OT  - cell aggregates
OT  - human bone marrow mesenchymal stromal cell
OT  - osteogenesis
EDAT- 2017/12/10 06:00
MHDA- 2019/11/14 06:00
CRDT- 2017/12/10 06:00
PHST- 2017/05/16 00:00 [received]
PHST- 2017/10/30 00:00 [revised]
PHST- 2017/11/29 00:00 [accepted]
PHST- 2017/12/10 06:00 [pubmed]
PHST- 2019/11/14 06:00 [medline]
PHST- 2017/12/10 06:00 [entrez]
AID - 10.1002/term.2625 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2018 Apr;12(4):e1936-e1949. doi: 10.1002/term.2625. Epub 
      2018 Jan 8.