PMID- 29223062
OWN - NLM
STAT- MEDLINE
DCOM- 20180130
LR  - 20180130
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 150
DP  - 2018 Feb 20
TI  - HPLC-MS/MS method optimisation for matrix metalloproteinase 3 and matrix 
      metalloproteinase 9 determination in human blood serum using target analysis.
PG  - 137-143
LID - S0731-7085(17)32220-3 [pii]
LID - 10.1016/j.jpba.2017.11.067 [doi]
AB  - A quantitative analysis of zinc endopeptidases matrix metalloproteinase 9 (MMP9) 
      and matrix metalloproteinase 3 (MMP3) from human blood serum are presented. Both 
      matrix metalloproteinases (MMP) are present in human blood serum and can be used 
      as biomarkers for different diseases. The analysis was performed using LC-MS/MS 
      with a triple quadrupole mass spectrometer, based on two specific peptides of 
      each MMP in comparison with an enzyme-linked immunosorbent assay (ELISA). While 
      the conditions for the LC-MS/MS analysis of MMP9 peptides were previously 
      reported for bronchoalveolar lavage fluid, the analysis of MMP3 peptides was 
      newly quantified for human blood serum herein for the first time. For MMP3, the 
      linear behaviour was determined in the concentration range from 1.0-200.0ng/mL 
      (R(2)=0.997) with an LLOD of 0.5ng/mL. For MMP9, linearity was determined in the 
      concentration range from 6.5-65.0ng/mL (R(2)=0.995) with an LLOD of 2.0ng/mL.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Kotnik, Petra
AU  - Kotnik P
AD  - Department of Chemistry, Faculty of Medicine, University of Maribor, Taborska 8, 
      SI-2000 Maribor, Slovenia; Laboratory for Separation Processes and Product 
      Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, 
      Smetanova 17, SI-2000 Maribor, Slovenia.
FAU - Krajnc, Metka Koren
AU  - Krajnc MK
AD  - Clinic of Internal Medicine, Department of Rheumatology, University Medical 
      Centre Maribor, Ljubljanska cesta 5, SI-2000 Maribor, Slovenia.
FAU - Pahor, Artur
AU  - Pahor A
AD  - Clinic of Internal Medicine, Department of Rheumatology, University Medical 
      Centre Maribor, Ljubljanska cesta 5, SI-2000 Maribor, Slovenia; Department of 
      Internal Medicine, Faculty of Medicine, University of Maribor, Taborska 8, 
      SI-2000 Maribor, Slovenia.
FAU - Finsgar, Matjaz
AU  - Finsgar M
AD  - Laboratory for Analytical Chemistry and Industrial Analysis, Faculty of Chemistry 
      and Chemical Engineering, University of Maribor, Smetanova 17, SI-2000 Maribor, 
      Slovenia.
FAU - Knez, Zeljko
AU  - Knez Z
AD  - Department of Chemistry, Faculty of Medicine, University of Maribor, Taborska 8, 
      SI-2000 Maribor, Slovenia; Laboratory for Separation Processes and Product 
      Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, 
      Smetanova 17, SI-2000 Maribor, Slovenia. Electronic address: zeljko.knez@um.si.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20171129
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Limit of Detection
MH  - Matrix Metalloproteinase 3/*blood
MH  - Matrix Metalloproteinase 9/*blood
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - ELISA
OT  - Human blood serum
OT  - LC-MS/MS
OT  - Matrix metalloproteinase 3
OT  - Matrix metalloproteinase 9
EDAT- 2017/12/10 06:00
MHDA- 2018/01/31 06:00
CRDT- 2017/12/10 06:00
PHST- 2017/09/01 00:00 [received]
PHST- 2017/11/21 00:00 [revised]
PHST- 2017/11/28 00:00 [accepted]
PHST- 2017/12/10 06:00 [pubmed]
PHST- 2018/01/31 06:00 [medline]
PHST- 2017/12/10 06:00 [entrez]
AID - S0731-7085(17)32220-3 [pii]
AID - 10.1016/j.jpba.2017.11.067 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2018 Feb 20;150:137-143. doi: 10.1016/j.jpba.2017.11.067. 
      Epub 2017 Nov 29.