PMID- 29227071
OWN - NLM
STAT- MEDLINE
DCOM- 20180202
LR  - 20180208
IS  - 0016-254X (Print)
IS  - 0016-254X (Linking)
VI  - 107
IP  - 12
DP  - 2016 Dec
TI  - [Natural Immunity and Ocular Inflammation].
PG  - 205-12
AB  - We have focused on the role of innate immunity during the formation ofochoroidal 
      neovascularization (CNV) -related diseasesu. Inflammation affects the formation 
      and the progression of various vitreoretinal diseases. We performed a 
      comprehensive analysis of inflammatory immune mediators in the vitreous fluids 
      with diabetic macular edema, proliferative diabetic retinopathy, branch retinal 
      vein occlusion, central retinal vein occlusion and rhegmatogenous retinal 
      detachment. The concentrations of 20 soluble factors (nine cytokines, six 
      chemokines, and five growth factors) were measured simultaneously by multiplex 
      bead analysis system. Out of 20 soluble factors, three factors : interleukin-6 
      (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) were 
      significantly elevated in all groups of vitreoretinal diseases compared with 
      control group. According to the correlation analysis in the individual patient's 
      level, these three factors that were simultaneously increased, did not show any 
      independent upregulation in all the examined diseases. We also elucidated the 
      role of natural killer (NK)T cells, which restricted CD1 molecule and participate 
      in the innate immune response, in laser-induced experimental CNV. We examined CNV 
      formation in independent two NKT cell-deficient mice, either CD1 knockout (KO) 
      mice or Ja18 KO mice, and found that both KO mice showed significant reduction of 
      experimental CNV. During the clinical process of CNV-related diseases, not only 
      CNV formation, subretinal scaring is thought to be another important step. We 
      thus established the experimental model of subretinal scaring by injecting 
      peritoneal exudating macrophages into subretinal space. Subretinal fibrous tissue 
      was observed by fundus scope in PEC-inoculated mice after seven days. The tissue 
      was consisted of monotonous and low cell-density area, which expressed a-SMA with 
      collagen synthesis. Because PEC-inoculated MCP-1 KO mice showed less amount of 
      glial residual, not only exogenous macrophages, but also intrinsic macrophages 
      are critical. Activated macrophages directly induced myofibrotic changes in RPE 
      cells in vitro.
FAU - Sonoda, Koh-Hei
AU  - Sonoda KH
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PT  - Review
PL  - Japan
TA  - Fukuoka Igaku Zasshi
JT  - Fukuoka igaku zasshi = Hukuoka acta medica
JID - 9423321
SB  - IM
MH  - Animals
MH  - Eye Diseases/diagnosis/*immunology
MH  - Humans
MH  - *Immunity, Innate
MH  - Inflammation/diagnosis/immunology
MH  - Lymphocytes/immunology
MH  - Neovascularization, Pathologic
EDAT- 2017/12/12 06:00
MHDA- 2018/02/03 06:00
CRDT- 2017/12/12 06:00
PHST- 2017/12/12 06:00 [entrez]
PHST- 2017/12/12 06:00 [pubmed]
PHST- 2018/02/03 06:00 [medline]
PST - ppublish
SO  - Fukuoka Igaku Zasshi. 2016 Dec;107(12):205-12.