PMID- 29228625 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220331 IS - 1949-2553 (Electronic) IS - 1949-2553 (Linking) VI - 8 IP - 57 DP - 2017 Nov 14 TI - Long noncoding RNA SFTA1P promoted apoptosis and increased cisplatin chemosensitivity via regulating the hnRNP-U-GADD45A axis in lung squamous cell carcinoma. PG - 97476-97489 LID - 10.18632/oncotarget.22138 [doi] AB - Chemotherapeutic insensitivity remains one of the major obstacles in clinical treatment of lung squamous cell carcinoma (LSCC). Recently, increasing evidence has suggested that long non-coding RNAs (lncRNAs) promote tumorigenesis in many cancer types. However, the potential biological roles and regulatory mechanisms of lncRNAs in response to cisplatin treatment are poorly understood. Here, we found that lncRNA SFTA1P (surfactant associated 1, pseudogene), highly expressed in lung, was down-regulated in LSCC tissues and could be induced upon cisplatin treatment in LSCC cells. Elevated SFTA1P induced apoptosis and enhanced the sensitivity to cisplatin of LSCC cells. We further identified that hnRNP-U (heterogeneous nuclear ribonucleoprotein U) was down-regulated in LSCCs and positively correlated with patients' poor prognosis as well as SFTA1P. Mechanistic studies revealed that SFTA1P could up-regulate hnRNP-U expression. In addition, we identified that hnRNP-U enhanced cisplatin-induced apoptosis through up-regulation of GADD45A, high expression of which was correlated with good prognosis in LSCC patients. Our findings demonstrated that SFTA1P might serve as a useful biomarker for LSCC diagnosis and a predictor for cisplatin chemotherapy response in patients with LSCC. FAU - Li, Ling AU - Li L AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - Yin, Ji-Ye AU - Yin JY AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - He, Fa-Zhong AU - He FZ AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - Huang, Ma-Sha AU - Huang MS AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - Zhu, Tao AU - Zhu T AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - Gao, Yuan-Feng AU - Gao YF AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - Chen, Yi-Xin AU - Chen YX AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - Zhou, Dong-Bo AU - Zhou DB AD - Department of Gerontology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. FAU - Chen, Xiang AU - Chen X AD - Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. FAU - Sun, Lun-Quan AU - Sun LQ AD - Center for Molecular Medicine, Xiangya Hospital, Key Laboratory of Molecular Radiation Oncology of Hunan Province, Central South University, Changsha 410008, P. R. China. FAU - Zhang, Wei AU - Zhang W AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - Zhou, Hong-Hao AU - Zhou HH AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. FAU - Liu, Zhao-Qian AU - Liu ZQ AD - Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, P. R. China. AD - Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, P. R. China. LA - eng PT - Journal Article DEP - 20171027 PL - United States TA - Oncotarget JT - Oncotarget JID - 101532965 PMC - PMC5722577 OTO - NOTNLM OT - apoptosis OT - cisplatin chemosensitivity OT - hnRNP-U- GADD45A OT - long noncoding RNA SFTA1P OT - lung squamous cell carcinoma COIS- CONFLICTS OF INTEREST All authors declare that no conflicts of interest exist. EDAT- 2017/12/13 06:00 MHDA- 2017/12/13 06:01 PMCR- 2017/11/14 CRDT- 2017/12/13 06:00 PHST- 2017/07/28 00:00 [received] PHST- 2017/08/24 00:00 [accepted] PHST- 2017/12/13 06:00 [entrez] PHST- 2017/12/13 06:00 [pubmed] PHST- 2017/12/13 06:01 [medline] PHST- 2017/11/14 00:00 [pmc-release] AID - 22138 [pii] AID - 10.18632/oncotarget.22138 [doi] PST - epublish SO - Oncotarget. 2017 Oct 27;8(57):97476-97489. doi: 10.18632/oncotarget.22138. eCollection 2017 Nov 14.