PMID- 29229370
OWN - NLM
STAT- MEDLINE
DCOM- 20180420
LR  - 20180420
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 517
DP  - 2018 Apr
TI  - Differences in signal peptide processing between GP3 glycoproteins of 
      Arteriviridae.
PG  - 69-76
LID - S0042-6822(17)30405-1 [pii]
LID - 10.1016/j.virol.2017.11.026 [doi]
AB  - We reported previously that carbohydrate attachment to an overlapping 
      glycosylation site adjacent to the signal peptide of GP3 from equine arteritis 
      virus (EAV) prevents cleavage. Here we investigated whether this unusual 
      processing scheme is a feature of GP3s of other Arteriviridae, which all contain 
      a glycosylation site at a similar position. Expression of GP3 from type-1 and 
      type-2 porcine reproductive and respiratory syndrome virus (PRRSV) and from 
      lactate dehydrogenase-elevating virus (LDV) revealed that the first glycosylation 
      site is used, but has no effect on signal peptide cleavage. Comparison of the 
      SDS-PAGE mobility of deglycosylated GP3 from PRRSV and LDV with mutants having or 
      not having a signal peptide showed that GP3 s signal peptide is cleaved. Swapping 
      the signal peptides between GP3 of EAV and PRRSV revealed that the information 
      for co-translational processing is not encoded in the signal peptide, but in the 
      remaining part of GP3.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Zhang, Minze
AU  - Zhang M
AD  - Institute of Virology, Free University Berlin, Robert von Ostertag-Strasse 7-13, 
      14163 Berlin, Germany.
FAU - Veit, Michael
AU  - Veit M
AD  - Institute of Virology, Free University Berlin, Robert von Ostertag-Strasse 7-13, 
      14163 Berlin, Germany. Electronic address: michael.veit@fu-berlin.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171208
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cricetinae
MH  - Gene Expression Regulation, Viral/*physiology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Signal Transduction
MH  - Viral Envelope Proteins/genetics/*metabolism
OTO - NOTNLM
OT  - Arteriviridae
OT  - Co-translational protein processing
OT  - Disulphide-linkage
OT  - EAV
OT  - GP3
OT  - LDV
OT  - N-glycosylation
OT  - PRRSV
OT  - Signal peptide cleavage
EDAT- 2017/12/13 06:00
MHDA- 2018/04/21 06:00
CRDT- 2017/12/13 06:00
PHST- 2017/09/06 00:00 [received]
PHST- 2017/11/27 00:00 [revised]
PHST- 2017/11/30 00:00 [accepted]
PHST- 2017/12/13 06:00 [pubmed]
PHST- 2018/04/21 06:00 [medline]
PHST- 2017/12/13 06:00 [entrez]
AID - S0042-6822(17)30405-1 [pii]
AID - 10.1016/j.virol.2017.11.026 [doi]
PST - ppublish
SO  - Virology. 2018 Apr;517:69-76. doi: 10.1016/j.virol.2017.11.026. Epub 2017 Dec 8.