PMID- 29231595
OWN - NLM
STAT- MEDLINE
DCOM- 20180622
LR  - 20181202
IS  - 0255-2930 (Print)
IS  - 0255-2930 (Linking)
VI  - 37
IP  - 4
DP  - 2017 Apr 12
TI  - [Effects of electroacupuncture on activation of microglia cells in spinal cord in 
      rats with neuropathic pain].
PG  - 411-416
LID - 10.13703/j.0255-2930.2017.04.016 [doi]
AB  - OBJECTIVE: To observe the effects of electroacupuncture (EA) on the activation of 
      microglia cells in the L(4) to L(6) spinal cord in rats with neuropathic pain, so 
      as to investigate whether EA could inhibit the activation of spinal microglial 
      cells and regulate the expression of brain-derived neurotrophic factor (BDNF) to 
      achieve the analgesic effect. METHODS: Forty male Sprague Dawley rats were 
      randomly divided into a normal group, a sham-model group, a model group and an EA 
      group, 10 rats in each one. The rats in the normal group received no treatment; 
      the rats in sham-model group were treated with operation to exposure sciatic 
      nerve for 2 to 3 min (no knot); the rats in the remaining groups were treated 
      with model establishment of chronic constrictive injury (CCI). 7 days after model 
      establishment, the rats in the EA group were treated with EA at "Zusanli" (ST 36) 
      and "Yanglingquan" (GB 34), 30 min per time, once a day for consecutive 7 days. 
      Only immobilization was used in the remaining groups the mechanical withdrawal 
      threshold (MWT) and thermal withdrawal latency (TWL) of affected side feet were 
      respectively measured before model establishment and 3 days, 5 days, 7 days, 10 
      days, 12 days and 14 days after model establishment; 14 days after model 
      establishment, rats were sacrificed; the immunohistochemical method was used to 
      measure the expression of Iba1 and BDNF in the sample of L(4) to L(6) spinal 
      cord; real-time fluorescent quantitative PCR was used to measure the expression 
      BDNF mRNA. RESULTS: Compared with the sham-model group, the pain threshold was 
      decreased significantly in the model group (P<0.05), leading to hyperpathia. 
      After EA treatment, compared with the model group, the pain threshold was 
      increased significantly in the EA group (P<0.05). 14 days after operation, the 
      microglia cells in the L(4) to L(6) spinal cord, expression of BDNF and level of 
      mRNA in the model group were significantly higher than those in the normal group 
      and sham-model group (all P<0.01); those in the EA group were significantly lower 
      than those in the model group (all P<0.01). CONCLUSIONS: The analgesic effect on 
      neuropathic pain is likely to be achieved by EA through inhibiting the activation 
      of spinal microglia cells and down-regulating the expression of BDNF.
FAU - Li, Sisi
AU  - Li S
AD  - Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325027, Zhejiang Province, China.
FAU - Gu, Pengpeng
AU  - Gu P
AD  - Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325027, Zhejiang Province, China.
FAU - Tu, Wenzhan
AU  - Tu W
AD  - Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325027, Zhejiang Province, China.
FAU - Jiang, Xia
AU  - Jiang X
AD  - Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325027, Zhejiang Province, China.
FAU - Chen, Wenci
AU  - Chen W
AD  - Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325027, Zhejiang Province, China.
FAU - Hu, Qimiao
AU  - Hu Q
AD  - Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325027, Zhejiang Province, China.
FAU - Jiang, Songhe
AU  - Jiang S
AD  - Department of Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou 325027, Zhejiang Province, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhen Jiu
JT  - Zhongguo zhen jiu = Chinese acupuncture & moxibustion
JID - 8600658
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Electroacupuncture/*methods
MH  - Lumbar Vertebrae
MH  - Male
MH  - Microglia/*physiology
MH  - Neuralgia/pathology/*therapy
MH  - Pain Threshold
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*pathology
OTO - NOTNLM
OT  - acupoint, GB34 (Yanglingquan)
OT  - acupoint, ST36 (Zusanli)
OT  - brain-derived neurotrophic factor (BDNF)
OT  - electroacupuncture (EA)
OT  - microglial cells
OT  - neuropathic pain
EDAT- 2017/12/13 06:00
MHDA- 2018/06/23 06:00
CRDT- 2017/12/13 06:00
PHST- 2017/12/13 06:00 [entrez]
PHST- 2017/12/13 06:00 [pubmed]
PHST- 2018/06/23 06:00 [medline]
AID - 10.13703/j.0255-2930.2017.04.016 [doi]
PST - ppublish
SO  - Zhongguo Zhen Jiu. 2017 Apr 12;37(4):411-416. doi: 
      10.13703/j.0255-2930.2017.04.016.