PMID- 29235553
OWN - NLM
STAT- MEDLINE
DCOM- 20190325
LR  - 20190325
IS  - 1476-5497 (Electronic)
IS  - 0307-0565 (Linking)
VI  - 42
IP  - 4
DP  - 2018 Apr
TI  - Pro-inflammatory gene expression profile in obese adults with high plasma GIP 
      levels.
PG  - 826-834
LID - 10.1038/ijo.2017.305 [doi]
AB  - BACKGROUND: Glucose-dependent insulinotropic peptide (GIP) provides a novel link 
      between the immune system and the gut, although results from different 
      experimental and observational studies are contradictory, ranging from 
      anti-inflammatory, through neutral to pro-inflammatory action of GIP. Thus, the 
      aim of this study was to analyze inflammatory pathways on the level of gene 
      expression and circulating inflammatory markers in relation to plasma GIP level. 
      SUBJECTS/METHODS: The study included 128 obese adults. Two groups of obese 
      subjects were created according to fasting GIP levels, with cutoff point at the 
      66th percentile and compared in respect with molecular and circulating markers of 
      inflammation. GIP, interleukin (IL)-6 and adipokines: leptin, adiponectin, 
      visfatin were measured by enzyme-linked immunosorbent assay. Inflammatory 
      markers: monocyte chemoattractant protein-1 (MCP-1), sE-Selectin, sVCAM-1, 
      sPECAM-1 were studied at fasting and after nutrient challenges. Gene expression 
      in blood cells was determined by human gene microarray. RESULTS: Obese patients 
      with high GIP levels had elevated fasting glucose (Q2 (Q1-Q3): 5.6 (5.0-6.0) vs 
      5.0 (4.8-5.4), P<0.001), homeostasis model assessment of insulin resistance (Q2 
      (Q1-Q3): 3.68 (2.72-5.42) vs 2.70 (2.13-4.33), P=0.021), thus increased markers 
      of insulin resistance as well as elevated inflammatory markers Il-6 (Q2 (Q1-Q3): 
      1.34 (1.0-2.04) vs 1.12 (0.76-1.64), P=0.045), MCP-1 (Q2 (Q1-Q3): 363 (287-447) 
      vs 323 (263-389), P=0.026). Leptin to adiponectin ratio was significantly 
      associated with fasting plasma GIP levels (beta (95% CI): 0.84 (0.10-1.59)) 
      independently of glucose levels. sE-Selectin was found to be a factor influencing 
      GIP response to oral glucose intake (beta (95% CI): 0.47 (0.14-0.81)) and sVCAM was 
      found to be a factor influencing GIP response to high-fat meal intake (beta (95% 
      CI): 0.19 (0.01-0.37)). We identified 32 genes of inflammatory pathways 
      differentially expressed in subjects with a high plasma GIP level compared to low 
      GIP. Most upregulated genes play a role in leukocyte chemotaxis and tissue 
      infiltration. CONCLUSIONS: These findings support the hypothesis that increased 
      GIP signaling has a role in chronic low-grade inflammation.
FAU - Goralska, J
AU  - Goralska J
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Razny, U
AU  - Razny U
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Polus, A
AU  - Polus A
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Stancel-Mozwillo, J
AU  - Stancel-Mozwillo J
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Chojnacka, M
AU  - Chojnacka M
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Gruca, A
AU  - Gruca A
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Zdzienicka, A
AU  - Zdzienicka A
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Dembinska-Kiec, A
AU  - Dembinska-Kiec A
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Kiec-Wilk, B
AU  - Kiec-Wilk B
AD  - Department of Metabolic Diseases, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Solnica, B
AU  - Solnica B
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
FAU - Malczewska-Malec, M
AU  - Malczewska-Malec M
AD  - Department of Clinical Biochemistry, Jagiellonian University Medical College, 
      Krakow, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171213
PL  - England
TA  - Int J Obes (Lond)
JT  - International journal of obesity (2005)
JID - 101256108
RN  - 0 (Adipokines)
RN  - 0 (Cytokines)
RN  - 59392-49-3 (Gastric Inhibitory Polypeptide)
SB  - IM
MH  - Adipokines/blood/genetics/*metabolism
MH  - Adult
MH  - Cohort Studies
MH  - Cytokines/blood/genetics/*metabolism
MH  - Female
MH  - Gastric Inhibitory Polypeptide/*blood/genetics/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Regulatory Networks
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Obesity/*blood/epidemiology/genetics/*metabolism
MH  - Transcriptome/*genetics
EDAT- 2017/12/14 06:00
MHDA- 2019/03/26 06:00
CRDT- 2017/12/14 06:00
PHST- 2017/02/02 00:00 [received]
PHST- 2017/11/22 00:00 [revised]
PHST- 2017/11/27 00:00 [accepted]
PHST- 2017/12/14 06:00 [pubmed]
PHST- 2019/03/26 06:00 [medline]
PHST- 2017/12/14 06:00 [entrez]
AID - ijo2017305 [pii]
AID - 10.1038/ijo.2017.305 [doi]
PST - ppublish
SO  - Int J Obes (Lond). 2018 Apr;42(4):826-834. doi: 10.1038/ijo.2017.305. Epub 2017 
      Dec 13.