PMID- 29236778
OWN - NLM
STAT- MEDLINE
DCOM- 20180116
LR  - 20190118
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 12
DP  - 2017
TI  - Interaction of porcine reproductive and respiratory syndrome virus proteins with 
      SUMO-conjugating enzyme reveals the SUMOylation of nucleocapsid protein.
PG  - e0189191
LID - 10.1371/journal.pone.0189191 [doi]
LID - e0189191
AB  - SUMOylation is a reversible post-translational modification that regulates the 
      function of target protein. In this study, we first predicted by software that 
      the multiple proteins of porcine reproductive and respiratory syndrome virus 
      (PRRSV) could be sumoylated. Next, we confirmed that Nsp1beta, Nsp4, Nsp9, Nsp10 and 
      nucleocapsid (N) protein of PRRSV could interact with the sole SUMO E2 
      conjugating enzyme Ubc9, and Ubc9 could be co-localized with Nsp1beta, Nsp4, Nsp9 
      and Nsp10 in the cytoplasm, while with N protein in both the cytoplasm and 
      nucleus. Finally, we demonstrated that N protein could be sumoylated by either 
      SUMO1 or SUMO2/3. In addition, the overexpression of Ubc9 could inhibit viral 
      genomic replication at early period of PRRSV infection and the knockdown of Ubc9 
      by siRNA could promote the virus replication. These findings reveal the 
      SUMOylation property of PRRSV N protein and the involvement of Ubc9 in PRRSV 
      replication through interaction with multiple proteins of PRRSV. To our 
      knowledge, this is the first study indicating the interplay between SUMO 
      modification system and PRRSV.
FAU - Wang, Cong
AU  - Wang C
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Zeng, Nanfang
AU  - Zeng N
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Liu, Siyu
AU  - Liu S
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Miao, Qi
AU  - Miao Q
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Ge, Xinna
AU  - Ge X
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Han, Jun
AU  - Han J
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Guo, Xin
AU  - Guo X
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
FAU - Yang, Hanchun
AU  - Yang H
AUID- ORCID: 0000-0003-3359-3925
AD  - Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of 
      Veterinary Medicine and State Key Laboratory of Agrobiotechnology, China 
      Agricultural University, Beijing, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20171213
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Nucleocapsid Proteins)
SB  - IM
MH  - Animals
MH  - Nucleocapsid Proteins/*metabolism
MH  - Porcine respiratory and reproductive syndrome virus/*metabolism/physiology
MH  - *Sumoylation
MH  - Swine
MH  - Two-Hybrid System Techniques
MH  - Virus Replication
PMC - PMC5728522
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/12/14 06:00
MHDA- 2018/01/18 06:00
PMCR- 2017/12/13
CRDT- 2017/12/14 06:00
PHST- 2017/07/20 00:00 [received]
PHST- 2017/11/21 00:00 [accepted]
PHST- 2017/12/14 06:00 [entrez]
PHST- 2017/12/14 06:00 [pubmed]
PHST- 2018/01/18 06:00 [medline]
PHST- 2017/12/13 00:00 [pmc-release]
AID - PONE-D-17-27218 [pii]
AID - 10.1371/journal.pone.0189191 [doi]
PST - epublish
SO  - PLoS One. 2017 Dec 13;12(12):e0189191. doi: 10.1371/journal.pone.0189191. 
      eCollection 2017.