PMID- 29239514
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20240313
IS  - 1751-7915 (Electronic)
IS  - 1751-7915 (Linking)
VI  - 11
IP  - 2
DP  - 2018 Mar
TI  - Analyses of Monascus pigment secretion and cellular morphology in non-ionic 
      surfactant micelle aqueous solution.
PG  - 409-419
LID - 10.1111/1751-7915.13038 [doi]
AB  - Monascus pigments produced by Monascus spp. are widely used as natural food 
      colourants. Extractive fermentation technology can facilitate the secretion of 
      intracellular Monascus pigments into extracellular non-ionic surfactant micelle 
      aqueous solution, so as to avoid the feedback inhibition and decomposition. In 
      this study, behaviour of the trans-membrane secretion of Monascus pigments was 
      investigated using morphological and spectroscopic analyses. Laser scanning 
      confocal microscopy (LSCM) traced that pigment secretion occurred through rapid 
      trans-membrane permeation in 4 min, with a simultaneous conversion in pigment 
      characteristics. Approximately 50% of intracellular pigments (AU(470) ) extracted 
      to extracellular broth with 40 g l(-1) Triton X-100, indicating the capacity for 
      pigment extraction was limited by the saturation concentrations of surfactant. 
      Scanning electron microscope (SEM) and transmission electron microscope (TEM) 
      imaging showed some damage in the cell wall but an intact cell membrane with a 
      slightly increased mycelial diameter. However, the physiological properties of 
      the cell membrane, including integrity, fluorescence intensity and permeability, 
      were altered. A diagram was provided to demonstrate the behaviour of Monascus 
      pigment secretion induced by Triton X-100. This study lays a foundation for the 
      further investigation of Monascus pigment metabolism and secretion in extractive 
      fermentation.
CI  - (c) 2018 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd 
      and Society for Applied Microbiology.
FAU - Chen, Gong
AU  - Chen G
AD  - School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of 
      Fermentation and Enzyme Engineering, South China University of Technology, 
      Guangzhou, 510006, China.
FAU - Wang, Meihua
AU  - Wang M
AD  - School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of 
      Fermentation and Enzyme Engineering, South China University of Technology, 
      Guangzhou, 510006, China.
FAU - Tian, Xiaofei
AU  - Tian X
AD  - School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of 
      Fermentation and Enzyme Engineering, South China University of Technology, 
      Guangzhou, 510006, China.
AD  - Dongguan Tianyi Biotechnology Co. Ltd., Dongguan, 523000, China.
FAU - Wu, Zhenqiang
AU  - Wu Z
AD  - School of Bioscience and Bioengineering, Guangdong Provincial Key Laboratory of 
      Fermentation and Enzyme Engineering, South China University of Technology, 
      Guangzhou, 510006, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20171214
PL  - United States
TA  - Microb Biotechnol
JT  - Microbial biotechnology
JID - 101316335
RN  - 0 (Food Coloring Agents)
RN  - 0 (Micelles)
RN  - 0 (Pigments, Biological)
RN  - 0 (Solutions)
RN  - 0 (Surface-Active Agents)
RN  - 9002-93-1 (Octoxynol)
SB  - IM
MH  - Food Coloring Agents/metabolism
MH  - *Micelles
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Monascus/*cytology/drug effects/*metabolism
MH  - Octoxynol/metabolism
MH  - Pigments, Biological/*metabolism
MH  - Solutions/*chemistry
MH  - Surface-Active Agents/*metabolism
PMC - PMC5812241
EDAT- 2017/12/15 06:00
MHDA- 2018/12/12 06:00
PMCR- 2017/12/14
CRDT- 2017/12/15 06:00
PHST- 2017/06/01 00:00 [received]
PHST- 2017/11/24 00:00 [revised]
PHST- 2017/11/27 00:00 [accepted]
PHST- 2017/12/15 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2017/12/15 06:00 [entrez]
PHST- 2017/12/14 00:00 [pmc-release]
AID - MBT213038 [pii]
AID - 10.1111/1751-7915.13038 [doi]
PST - ppublish
SO  - Microb Biotechnol. 2018 Mar;11(2):409-419. doi: 10.1111/1751-7915.13038. Epub 
      2017 Dec 14.