PMID- 29240779
OWN - NLM
STAT- MEDLINE
DCOM- 20180116
LR  - 20240327
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 12
IP  - 12
DP  - 2017
TI  - Impact of maternal high fat diet on hypothalamic transcriptome in neonatal 
      Sprague Dawley rats.
PG  - e0189492
LID - 10.1371/journal.pone.0189492 [doi]
LID - e0189492
AB  - Maternal consumption of a high fat diet during early development has been shown 
      to impact the formation of hypothalamic neurocircuitry, thereby contributing to 
      imbalances in appetite and energy homeostasis and increasing the risk of obesity 
      in subsequent generations. Early in postnatal life, the neuronal projections 
      responsible for energy homeostasis develop in response to appetite-related 
      peptides such as leptin. To date, no study characterises the genome-wide 
      transcriptional changes that occur in response to exposure to high fat diet 
      during this critical window. We explored the effects of maternal high fat diet 
      consumption on hypothalamic gene expression in Sprague Dawley rat offspring at 
      postnatal day 10. RNA-sequencing enabled discovery of differentially expressed 
      genes between offspring of dams fed a high fat diet and offspring of control diet 
      fed dams. Female high fat diet offspring displayed altered expression of 86 genes 
      (adjusted P-value<0.05), including genes coding for proteins of the extra 
      cellular matrix, particularly Collagen 1a1 (Col1a1), Col1a2, Col3a1, and the 
      imprinted Insulin-like growth factor 2 (Igf2) gene. Male high fat diet offspring 
      showed significant changes in collagen genes (Col1a1 and Col3a1) and significant 
      upregulation of two genes involved in regulation of dopamine availability in the 
      brain, tyrosine hydroxylase (Th) and dopamine reuptake transporter Slc6a3 (also 
      known as Dat1). Transcriptional changes were accompanied by increased body 
      weight, body fat and body length in the high fat diet offspring, as well as 
      altered blood glucose and plasma leptin. Transcriptional changes identified in 
      the hypothalamus of offspring of high fat diet mothers could alter neuronal 
      projection formation during early development leading to abnormalities in the 
      neuronal circuitry controlling appetite in later life, hence priming offspring to 
      the development of obesity.
FAU - Barrand, Sanna
AU  - Barrand S
AUID- ORCID: 0000-0001-5642-5196
AD  - Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, 
      Australia.
FAU - Crowley, Tamsyn M
AU  - Crowley TM
AD  - Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, 
      Australia.
AD  - MMR, BCRG, School of Medicine, Deakin University, Waurn Ponds, Victoria, 
      Australia.
FAU - Wood-Bradley, Ryan J
AU  - Wood-Bradley RJ
AD  - Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, 
      Australia.
FAU - De Jong, Kirstie A
AU  - De Jong KA
AD  - Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, 
      Australia.
FAU - Armitage, James A
AU  - Armitage JA
AD  - Faculty of Health, School of Medicine, Deakin University, Waurn Ponds, Victoria, 
      Australia.
LA  - eng
PT  - Journal Article
DEP - 20171214
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Animals
MH  - *Animals, Newborn
MH  - *Diet, High-Fat
MH  - Female
MH  - Hypothalamus/*metabolism
MH  - Phenotype
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Transcriptome
PMC - PMC5730210
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2017/12/15 06:00
MHDA- 2018/01/18 06:00
PMCR- 2017/12/14
CRDT- 2017/12/15 06:00
PHST- 2017/06/07 00:00 [received]
PHST- 2017/11/27 00:00 [accepted]
PHST- 2017/12/15 06:00 [entrez]
PHST- 2017/12/15 06:00 [pubmed]
PHST- 2018/01/18 06:00 [medline]
PHST- 2017/12/14 00:00 [pmc-release]
AID - PONE-D-17-21729 [pii]
AID - 10.1371/journal.pone.0189492 [doi]
PST - epublish
SO  - PLoS One. 2017 Dec 14;12(12):e0189492. doi: 10.1371/journal.pone.0189492. 
      eCollection 2017.